PMID- 35509492
OWN - NLM
STAT- MEDLINE
DCOM- 20220506
LR  - 20220716
IS  - 1177-8881 (Electronic)
IS  - 1177-8881 (Linking)
VI  - 16
DP  - 2022
TI  - Effects of Cartilage Progenitor Cells, Bone Marrow Mesenchymal Stem Cells and 
      Chondrocytes on Cartilage Repair as Seed Cells: An in vitro Study.
PG  - 1217-1230
LID - 10.2147/DDDT.S356936 [doi]
AB  - PURPOSE: To determine the effects of cartilage progenitor cells, bone marrow 
      mesenchymal stem cells and chondrocytes on cartilage repair as seed cells. 
      METHODS: Porcine cartilage progenitor cells (CPCs), bone marrow mesenchymal stem 
      cells (BMSCs) and chondrocytes (CCs) were obtained from the femoropatellar joints 
      of young pigs, and seeded in agarose gel as a graft. During the 28-day culture, 
      proliferation ability was measured by MTT assay, and gene expression of Collagen 
      I, Collagen II, Aggrecan and SOX 9 were measured by qPCR. Qualitative and 
      quantitative analysis of collagen, glycosaminoglycan and DNA were appraised by 
      immunohistochemical staining and biochemical assay, and integration strength was 
      analyzed by push-out tests. RESULTS: After 28-day culture, proliferation ability 
      of CPCs and BMSCs was higher than CCs. Collagen, glycosaminoglycan, DNA content 
      and chondrocyte-related genes expression in the cartilage progenitor cells seeded 
      gel were significantly higher than the other two gels. Integration strength in 
      the cartilage progenitor cells seeded gel was also higher compared with the other 
      two gels. CONCLUSION: Compared with CCs and BMSCs, CPCs in vitro have dominance 
      in the ability of cell proliferation and differentiation as seed cells in tissue 
      engineering.
CI  - (c) 2022 Gu et al.
FAU - Gu, Jiaxiang
AU  - Gu J
AD  - Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of 
      China.
AD  - Department of Foot and Hand Surgery, Northern Jiangsu People's Hospital, 
      Yangzhou, People's Republic of China.
FAU - Wang, Bin
AU  - Wang B
AD  - Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of 
      China.
AD  - Department of Foot and Hand Surgery, Northern Jiangsu People's Hospital, 
      Yangzhou, People's Republic of China.
FAU - Wang, Tianliang
AU  - Wang T
AD  - Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of 
      China.
AD  - Department of Foot and Hand Surgery, Northern Jiangsu People's Hospital, 
      Yangzhou, People's Republic of China.
FAU - Zhang, Naichen
AU  - Zhang N
AD  - Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of 
      China.
AD  - Department of Foot and Hand Surgery, Northern Jiangsu People's Hospital, 
      Yangzhou, People's Republic of China.
FAU - Liu, Hongjun
AU  - Liu H
AD  - Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of 
      China.
AD  - Department of Foot and Hand Surgery, Northern Jiangsu People's Hospital, 
      Yangzhou, People's Republic of China.
FAU - Gui, Jianchao
AU  - Gui J
AD  - Department of Orthopedics, Nanjing Medical University Affiliated Nanjing First 
      Hospital, Nanjing, People's Republic of China.
FAU - Lu, Yiming
AU  - Lu Y
AUID- ORCID: 0000-0002-8983-5957
AD  - Clinical Medical College, Yangzhou University, Yangzhou, People's Republic of 
      China.
AD  - Department of Foot and Hand Surgery, Northern Jiangsu People's Hospital, 
      Yangzhou, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20220428
PL  - New Zealand
TA  - Drug Des Devel Ther
JT  - Drug design, development and therapy
JID - 101475745
RN  - 0 (Glycosaminoglycans)
RN  - 9007-34-5 (Collagen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cartilage/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - *Chondrocytes/metabolism
MH  - Collagen
MH  - DNA
MH  - Glycosaminoglycans/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Stem Cells
MH  - Swine
PMC - PMC9059879
OTO - NOTNLM
OT  - cartilage progenitor cells
OT  - cartilage repair
OT  - tissue engineering
COIS- The authors declare that they have no competing interests.
EDAT- 2022/05/06 06:00
MHDA- 2022/05/07 06:00
PMCR- 2022/04/28
CRDT- 2022/05/05 02:26
PHST- 2022/01/03 00:00 [received]
PHST- 2022/04/11 00:00 [accepted]
PHST- 2022/05/05 02:26 [entrez]
PHST- 2022/05/06 06:00 [pubmed]
PHST- 2022/05/07 06:00 [medline]
PHST- 2022/04/28 00:00 [pmc-release]
AID - 356936 [pii]
AID - 10.2147/DDDT.S356936 [doi]
PST - epublish
SO  - Drug Des Devel Ther. 2022 Apr 28;16:1217-1230. doi: 10.2147/DDDT.S356936. 
      eCollection 2022.