PMID- 35511336
OWN - NLM
STAT- MEDLINE
DCOM- 20220919
LR  - 20220929
IS  - 2212-5469 (Electronic)
IS  - 1738-2696 (Print)
IS  - 1738-2696 (Linking)
VI  - 19
IP  - 5
DP  - 2022 Oct
TI  - A Comprehensive Cancer-Associated MicroRNA Expression Profiling and Proteomic 
      Analysis of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes.
PG  - 1013-1031
LID - 10.1007/s13770-022-00450-8 [doi]
AB  - BACKGROUND: The mesenchymal stem cells (MSCs) have enormous therapeutic potential 
      owing to their multi-lineage differentiation and self-renewal properties. MSCs 
      express growth factors, cytokines, chemokines, and non-coding regulatory RNAs 
      with immunosuppressive, anti-tumor, and migratory properties. MSCs also release 
      several anti-cancer molecules via extracellular vesicles, that act as 
      pro-apoptotic/tumor suppressor factors. This study aimed to identify the stem 
      cell-derived secretome that could exhibit anti-cancer properties through 
      molecular profiling of cargos in MSC-derived exosomes. METHODS: Human umbilical 
      cord mesenchymal stem cells (hUCMSCs) were isolated from umbilical cord tissues 
      and culture expanded. Subsequently, exosomes were isolated from hUCMSC 
      conditioned medium and characterized by DLS, electron microscopy. Western blot 
      for exosome surface marker protein CD63 expression was performed. The miRNA 
      profiling of hUCMSCs and hUCMSC-derived exosomes was performed, followed by 
      functional enrichment analysis. RESULTS: The tri-lineage differentiation 
      potential, fibroblastic morphology, and strong expression of pluripotency genes 
      indicated that isolated fibroblasts are MSCs. The isolated extracellular vesicles 
      were 133.8 +/- 42.49 nm in diameter, monodispersed, and strongly expressed the 
      exosome surface marker protein CD63. The miRNA expression profile and gene 
      ontology (GO) depicted the differential expression patterns of high and 
      less-expressed miRNAs that are crucial to be involved in the regulation of 
      apoptosis. The LCMS/MS data and GO analysis indicate that hUCMSC secretomes are 
      involved in several oncogenic and inflammatory signaling cascades. CONCLUSION: 
      Primary human MSCs released miRNAs and growth factors via exosomes that are 
      increasingly implicated in intercellular communications, and hUCMSC-exosomal 
      miRNAs have a critical influence in regulating cell death and apoptosis of cancer 
      cells.
CI  - (c) 2022. The Author(s).
FAU - Jothimani, Ganesan
AU  - Jothimani G
AD  - Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad 
      Hospital and Research Institute (CHRI), Chettinad Academy of Research and 
      Education (CARE), Kelambakkam, Chennai, Tamil Nadu, 603 103, India.
FAU - Pathak, Surajit
AU  - Pathak S
AD  - Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad 
      Hospital and Research Institute (CHRI), Chettinad Academy of Research and 
      Education (CARE), Kelambakkam, Chennai, Tamil Nadu, 603 103, India.
FAU - Dutta, Suman
AU  - Dutta S
AD  - International Institute of Innovation and Technology, DH Block, Action Area 1D, 
      Newtown, Kolkata, West Bengal, 700156, India.
FAU - Duttaroy, Asim K
AU  - Duttaroy AK
AUID- ORCID: 0000-0003-1619-3778
AD  - Department of Nutrition, Institute of Basic Medical Sciences, Faculty of 
      Medicine, University of Oslo, Oslo, Norway. a.k.duttaroy@medisin.uio.no.
FAU - Banerjee, Antara
AU  - Banerjee A
AUID- ORCID: 0000-0002-5519-6878
AD  - Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad 
      Hospital and Research Institute (CHRI), Chettinad Academy of Research and 
      Education (CARE), Kelambakkam, Chennai, Tamil Nadu, 603 103, India. 
      antarabanerjee@care.edu.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220505
PL  - Korea (South)
TA  - Tissue Eng Regen Med
JT  - Tissue engineering and regenerative medicine
JID - 101699923
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytokines/metabolism
MH  - *Exosomes/metabolism
MH  - Humans
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Neoplasms/metabolism
MH  - Proteomics
MH  - Umbilical Cord
PMC - PMC9478013
OTO - NOTNLM
OT  - Cancer
OT  - Exosomes
OT  - Gene ontology
OT  - Mesenchymal stem cells
OT  - Proteome
OT  - Stem cell therapeutics
OT  - miRNA
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/05/06 06:00
MHDA- 2022/09/20 06:00
PMCR- 2022/05/05
CRDT- 2022/05/05 11:59
PHST- 2021/12/27 00:00 [received]
PHST- 2022/03/04 00:00 [accepted]
PHST- 2022/02/23 00:00 [revised]
PHST- 2022/05/06 06:00 [pubmed]
PHST- 2022/09/20 06:00 [medline]
PHST- 2022/05/05 11:59 [entrez]
PHST- 2022/05/05 00:00 [pmc-release]
AID - 10.1007/s13770-022-00450-8 [pii]
AID - 450 [pii]
AID - 10.1007/s13770-022-00450-8 [doi]
PST - ppublish
SO  - Tissue Eng Regen Med. 2022 Oct;19(5):1013-1031. doi: 10.1007/s13770-022-00450-8. 
      Epub 2022 May 5.