PMID- 35513070
OWN - NLM
STAT- MEDLINE
DCOM- 20220629
LR  - 20220716
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 298
IP  - 6
DP  - 2022 Jun
TI  - Deformation of caveolae impacts global transcription and translation processes 
      through relocalization of cavin-1.
PG  - 102005
LID - S0021-9258(22)00445-8 [pii]
LID - 10.1016/j.jbc.2022.102005 [doi]
LID - 102005
AB  - Caveolae are invaginated membrane domains that provide mechanical strength to 
      cells in addition to being focal points for the localization of signaling 
      molecules. Caveolae are formed through the aggregation of caveolin-1 or -3 
      (Cav1/3), membrane proteins that assemble into multifunctional complexes with the 
      help of caveola-associated protein cavin-1. In addition to its role in the 
      formation of caveolae, cavin-1, also called polymerase I and transcript release 
      factor, is further known to promote ribosomal RNA transcription in the nucleus. 
      However, the mechanistic link between these functions is not clear. Here, we 
      found that deforming caveolae by subjecting cells to mild osmotic stress (150-300 
      mOsm) changes levels of GAPDH, Hsp90, and Ras only when Cav1/cavin-1 levels are 
      reduced, suggesting a link between caveola deformation and global protein 
      expression. We show that this link may be due to relocalization of cavin-1 to the 
      nucleus upon caveola deformation. Cavin-1 relocalization is also seen when 
      Cav1-Galphaq contacts change upon stimulation. Furthermore, Cav1 and cavin-1 levels 
      have been shown to have profound effects on cytosolic RNA levels, which in turn 
      impact the ability of cells to form stress granules and RNA-processing bodies 
      (p-bodies) which sequester and degrade mRNAs, respectively. Our studies here 
      using a cavin-1-knockout cell line indicate adaptive changes in cytosolic RNA 
      levels but a reduced ability to form stress granules. Taken together, our 
      findings suggest that caveolae, through release of cavin-1, communicate 
      extracellular cues to the cell interior to impact transcriptional and 
      translational.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Qifti, Androniqi
AU  - Qifti A
AD  - Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 
      Worcester, Massachusetts, USA.
FAU - Balaji, Shravani
AU  - Balaji S
AD  - Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 
      Worcester, Massachusetts, USA.
FAU - Scarlata, Suzanne
AU  - Scarlata S
AD  - Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 
      Worcester, Massachusetts, USA. Electronic address: sfscarlata@wpi.edu.
LA  - eng
GR  - R01 GM116178/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220502
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Caveolin 1)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - *Caveolae/metabolism/pathology
MH  - *Caveolin 1/genetics/metabolism
MH  - Cell Line
MH  - Gene Knockout Techniques
MH  - Membrane Proteins/metabolism
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/metabolism
MH  - *RNA-Binding Proteins/genetics/metabolism
MH  - Signal Transduction
MH  - *Transcription, Genetic
PMC - PMC9168624
OTO - NOTNLM
OT  - caveola domains
OT  - cavin-1
OT  - cell stress
OT  - nuclear relocalization
OT  - protein translation
COIS- Conflict of interest The authors declare that they have no conflicts of interest 
      with the contents of this article.
EDAT- 2022/05/06 06:00
MHDA- 2022/06/30 06:00
PMCR- 2022/05/02
CRDT- 2022/05/05 21:45
PHST- 2021/12/21 00:00 [received]
PHST- 2022/04/16 00:00 [revised]
PHST- 2022/04/19 00:00 [accepted]
PHST- 2022/05/06 06:00 [pubmed]
PHST- 2022/06/30 06:00 [medline]
PHST- 2022/05/05 21:45 [entrez]
PHST- 2022/05/02 00:00 [pmc-release]
AID - S0021-9258(22)00445-8 [pii]
AID - 102005 [pii]
AID - 10.1016/j.jbc.2022.102005 [doi]
PST - ppublish
SO  - J Biol Chem. 2022 Jun;298(6):102005. doi: 10.1016/j.jbc.2022.102005. Epub 2022 
      May 2.