PMID- 35513090
OWN - NLM
STAT- MEDLINE
DCOM- 20220531
LR  - 20220531
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 210
DP  - 2022 Jun 15
TI  - Efficient production of inulo-oligosaccharides from inulin by exo- and 
      endo-inulinase co-immobilized onto a self-assembling protein scaffold.
PG  - 588-599
LID - S0141-8130(22)00933-3 [pii]
LID - 10.1016/j.ijbiomac.2022.04.213 [doi]
AB  - Inulin can be hydrolyzed by inulinases to yield inulo-oligosaccharides (IOSs), 
      which have great application potential in the food and nutraceutical industries. 
      However, conventional enzymatic production of IOSs is limited by long hydrolysis 
      times and poor thermo-stability of inulinases. Here, the self-assembling protein 
      scaffold EutM was engineered to co-immobilize exo-inulinase (EXINU) and 
      endo-inulinase (ENINU) for synergistic hydrolysis of inulin to produce IOSs with 
      3 to 5 monosaccharide units (DP3-5 IOSs). The immobilization of EXINU/ENINU onto 
      the EutM scaffold resulted in an increase of catalytic efficiency, a 65% increase 
      of the V(max) of ENINU, as well as an increase of thermo-stability, with 
      4.26-fold higher residual activity of EXINU after 22 h-incubation at 50  degrees C. After 
      optimization, two efficient production protocols were obtained, in which the 
      yield and productivity of DP3-5 IOSs reached 80.38% and 70.86 g.(L.h)(-1), 
      respectively, which were at a high level in similar studies. Overall, this study 
      provides an attractive self-assembling protein platform for the co-immobilization 
      of inulinases, as well as optimized bioprocesses with great promise for the 
      industrial production of DP3-5 IOSs.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Chen, Xinyi
AU  - Chen X
AD  - College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 
      Nanjing, Jiangsu 211816, China.
FAU - Chen, Xianhan
AU  - Chen X
AD  - College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 
      Nanjing, Jiangsu 211816, China.
FAU - Zhu, Liying
AU  - Zhu L
AD  - School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 
      Jiangsu, 210009, China.
FAU - Liu, Wei
AU  - Liu W
AD  - College of Food Science and Light Industry, State Key Laboratory of 
      Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 
      Jiangsu 211816, China.. Electronic address: liuwei6775@njtech.edu.cn.
FAU - Jiang, Ling
AU  - Jiang L
AD  - College of Food Science and Light Industry, State Key Laboratory of 
      Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 
      Jiangsu 211816, China.. Electronic address: jiangling@njtech.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220502
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Oligosaccharides)
RN  - 9005-80-5 (Inulin)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.7 (inulinase)
SB  - IM
MH  - *Glycoside Hydrolases/metabolism
MH  - Hydrolysis
MH  - *Inulin/metabolism
MH  - Oligosaccharides/metabolism
OTO - NOTNLM
OT  - Co-immobilization
OT  - Inulinase
OT  - Inulo-oligosaccharides
OT  - Protein scaffold
OT  - Response surface methodology
OT  - Synergistic effect
EDAT- 2022/05/06 06:00
MHDA- 2022/06/01 06:00
CRDT- 2022/05/05 21:45
PHST- 2022/02/28 00:00 [received]
PHST- 2022/04/19 00:00 [revised]
PHST- 2022/04/28 00:00 [accepted]
PHST- 2022/05/06 06:00 [pubmed]
PHST- 2022/06/01 06:00 [medline]
PHST- 2022/05/05 21:45 [entrez]
AID - S0141-8130(22)00933-3 [pii]
AID - 10.1016/j.ijbiomac.2022.04.213 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2022 Jun 15;210:588-599. doi: 
      10.1016/j.ijbiomac.2022.04.213. Epub 2022 May 2.