PMID- 35515210
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 9
IP  - 32
DP  - 2019 Jun 10
TI  - The effect of nanoencapsulation of ICG on two-photon bioimaging.
PG  - 18703-18712
LID - 10.1039/c9ra03152a [doi]
AB  - Multiphoton imaging, a highly effective diagnostic technique, has recently gained 
      widespread attention for early-stage cancer detection. Tremendous efforts have 
      been dedicated to explore various types of exogenous contrast agents for improved 
      signal-to-noise ratio of multiphoton imaging. Indocyanine green (ICG), the only 
      U. S. FDA approved near-infrared chromophore, has been recently used as an 
      exogenous contrast agent for two-photon bioimaging. Despite its great potential 
      applications in clinical settings, the conventional delivery method of ICG has 
      limited applications due to its poor cellular uptake and optical stability in its 
      free form. Herein, we report the effect of nanoencapsulation of ICG on two-photon 
      bioimaging. For this study, ICG was encapsulated within poly-l-arginine (PLA) 
      based nanoparticles for the first time. These nanoparticles were found to be 
      biocompatible and biodegradable as the major constituents were salts and PLA. 
      These nanoparticles were spherical with a mean diameter of  approximately 61 nm and exhibit 
      higher photostability than free ICG. Additionally, nanoencapsulated ICG treated 
      cells show enhanced contrast for two-photon bioimaging in comparison with its 
      free form. In summary, nanoencapsulated ICG could serve as an exogenous 
      chromophore for multiphoton imaging, which shows excellent delivery efficacy.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Kumari, Anshu
AU  - Kumari A
AD  - Discipline of Biosciences and Biomedical Engineering, Indian Institute of 
      Technology Indore Indore India-453552 shgupta@iiti.ac.in +91-732-4306743.
FAU - Kumari, Kalpana
AU  - Kumari K
AD  - Discipline of Biosciences and Biomedical Engineering, Indian Institute of 
      Technology Indore Indore India-453552 shgupta@iiti.ac.in +91-732-4306743.
FAU - Gupta, Sharad
AU  - Gupta S
AUID- ORCID: 0000-0003-2521-0170
AD  - Discipline of Biosciences and Biomedical Engineering, Indian Institute of 
      Technology Indore Indore India-453552 shgupta@iiti.ac.in +91-732-4306743.
AD  - Metallurgy Engineering and Materials Science, Indian Institute of Technology 
      Indore Indore India-453552.
LA  - eng
PT  - Journal Article
DEP - 20190613
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9064784
COIS- There are no conflicts to declare.
EDAT- 2019/06/13 00:00
MHDA- 2019/06/13 00:01
PMCR- 2019/06/13
CRDT- 2022/05/06 05:33
PHST- 2019/04/27 00:00 [received]
PHST- 2019/06/07 00:00 [accepted]
PHST- 2022/05/06 05:33 [entrez]
PHST- 2019/06/13 00:00 [pubmed]
PHST- 2019/06/13 00:01 [medline]
PHST- 2019/06/13 00:00 [pmc-release]
AID - c9ra03152a [pii]
AID - 10.1039/c9ra03152a [doi]
PST - epublish
SO  - RSC Adv. 2019 Jun 13;9(32):18703-18712. doi: 10.1039/c9ra03152a. eCollection 2019 
      Jun 10.