PMID- 35515389
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 10
IP  - 65
DP  - 2020 Oct 27
TI  - Integrated glycomics strategy for the evaluation of glycosylation alterations in 
      salivary proteins associated with type 2 diabetes mellitus.
PG  - 39739-39752
LID - 10.1039/d0ra05466f [doi]
AB  - Glycosylation is involved in several biological processes, and its alterations 
      can reflect the process of certain diseases. Type 2 diabetes mellitus (T2DM) has 
      attained the status of a global pandemic; however, the difference in salivary 
      protein glycosylation between healthy subjects and patients with T2DM has not 
      been fully understood. In the present study, salivary specimens from patients 
      with T2DM (n = 72) and healthy volunteers (HVs, n = 80) were enrolled and divided 
      into discovery and validation cohorts. A method combining the lectin microarray 
      and lectin blotting was employed to investigate and confirm the altered 
      glycopatterns in salivary glycoproteins. Then, lectin-mediated affinity capture 
      of glycoproteins and MALDI-TOF/TOF-MS were performed to obtain the precise 
      structural information of the altered glycans. As a result, the glycopatterns 
      recognized by 5 lectins (LEL, VVA, Jacalin, RCA120 and DSA) showed significant 
      alteration in the saliva of T2DM patients. Notably, the glycopattern of 
      Galbeta-1,4GlcNAc (LacNAc) recognized by LEL exhibited a significant increase in 
      T2DM patients compared to HVs in both discovery and validation cohorts. The 
      MALDI-TOF/TOF-MS results indicated that there were 10 and 7 LacNAc-containing 
      N/O-glycans (e.g. m/z 1647.586, 11 688.613 and 1562.470) that were identified 
      only in T2DM patients. Besides, the relative abundance of 3 LacNAc-containing 
      N-glycans and 10 LacNAc-containing O-glycans showed an increase in the 
      glycopattern in T2DM patients. These results indicated that the glycopattern of 
      LacNAc is increased in salivary glycoproteins from T2DM patients, and an increase 
      in LacNAc-containing N/O-glycans may contribute to this alteration. Our findings 
      provide useful information to understand the complex physiological changes in the 
      T2DM patients.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Yu, Hanjie
AU  - Yu H
AUID- ORCID: 0000-0003-2684-8715
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Wang, Junhong
AU  - Wang J
AD  - Department of Endocrinology, Second Affiliated Hospital of Xi'an Jiaotong 
      University Xi'an 710004 China.
FAU - Tang, Zhen
AU  - Tang Z
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Li, Xia
AU  - Li X
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Yin, Mengqi
AU  - Yin M
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Shu, Jian
AU  - Shu J
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Chen, Wentian
AU  - Chen W
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
FAU - Yang, Shuang
AU  - Yang S
AUID- ORCID: 0000-0001-7958-0594
AD  - Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Soochow 
      University Suzhou Jiangsu China Yangs2020@suda.edu.cn.
FAU - Li, Zheng
AU  - Li Z
AD  - Laboratory for Functional Glycomics, College of Life Sciences, Northwest 
      University No. 229 Taibai Beilu Xi'an 710069 China zhengli@nwu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20201101
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9057417
COIS- The authors declare no conflict of interest.
EDAT- 2020/11/01 00:00
MHDA- 2020/11/01 00:01
PMCR- 2020/11/01
CRDT- 2022/05/06 05:35
PHST- 2020/06/22 00:00 [received]
PHST- 2020/10/10 00:00 [accepted]
PHST- 2022/05/06 05:35 [entrez]
PHST- 2020/11/01 00:00 [pubmed]
PHST- 2020/11/01 00:01 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - d0ra05466f [pii]
AID - 10.1039/d0ra05466f [doi]
PST - epublish
SO  - RSC Adv. 2020 Nov 1;10(65):39739-39752. doi: 10.1039/d0ra05466f. eCollection 2020 
      Oct 27.