PMID- 35516628
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231102
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 10
IP  - 37
DP  - 2020 Jun 8
TI  - Purification, characterization, and hypoglycemic properties of eurocristatine 
      from Eurotium cristatum spores in Fuzhuan brick tea.
PG  - 22234-22241
LID - 10.1039/d0ra03423a [doi]
AB  - Fuzhuan brick tea (FBT) is a Chinese dark tea that is famous for its significant 
      health benefits, in which Eurotium cristatum (E. cristatum) strains play a vital 
      role in its postfermentation process. In this study, eurocristatine with 
      hypoglycemic activity was discovered for the first time and purified from the 
      spores of E. cristatum growing in FBT. Eurocristatine (98%) was obtained by D-101 
      macroporous resin-based column chromatography and preparative high performance 
      liquid chromatography (HPLC) with a C(18) column as the stationary phase and 35% 
      acetonitrile in ultrapure water as the mobile phase. Hypoglycemic activity in a 
      Hep-G2 cell hypoglycemic model was used as a screening indicator during 
      purification. The chemical structure of eurocristatine was characterized by 
      ESI/MS, (1)H NMR and (13)C NMR analyses. The antidiabetic effects of 
      eurocristatine were verified in high-fat diet/streptozocin-induced type 2 
      diabetes mellitus (T2DM) rats. The results showed that eurocristatine 
      significantly reduced fasting blood glucose. Our study demonstrated that 
      eurocristatine, as a newly discovered hypoglycemic active substance, could be 
      considered a potential candidate for the treatment of diabetes and its 
      complications.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Liu, Gang
AU  - Liu G
AD  - Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical 
      Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 
      China zch2005@nwu.edu.cn +86-29-88305118 +86-29-88305118.
AD  - Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of 
      Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 
      710069 China.
AD  - Biotech & Biomed Research Institute, Northwest University Taibai North Road 229 
      Xi'an Shaanxi 710069 China.
FAU - Duan, Zhiguang
AU  - Duan Z
AD  - Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical 
      Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 
      China zch2005@nwu.edu.cn +86-29-88305118 +86-29-88305118.
AD  - Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of 
      Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 
      710069 China.
AD  - Biotech & Biomed Research Institute, Northwest University Taibai North Road 229 
      Xi'an Shaanxi 710069 China.
FAU - Wang, Pan
AU  - Wang P
AD  - Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical 
      Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 
      China zch2005@nwu.edu.cn +86-29-88305118 +86-29-88305118.
AD  - Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of 
      Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 
      710069 China.
AD  - Biotech & Biomed Research Institute, Northwest University Taibai North Road 229 
      Xi'an Shaanxi 710069 China.
FAU - Fan, Daidi
AU  - Fan D
AUID- ORCID: 0000-0001-9798-1674
AD  - Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical 
      Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 
      China zch2005@nwu.edu.cn +86-29-88305118 +86-29-88305118.
AD  - Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of 
      Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 
      710069 China.
AD  - Biotech & Biomed Research Institute, Northwest University Taibai North Road 229 
      Xi'an Shaanxi 710069 China.
FAU - Zhu, Chenhui
AU  - Zhu C
AUID- ORCID: 0000-0002-7452-2288
AD  - Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical 
      Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 710069 
      China zch2005@nwu.edu.cn +86-29-88305118 +86-29-88305118.
AD  - Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of 
      Chemical Engineering, Northwest University Taibai North Road 229 Xi'an Shaanxi 
      710069 China.
AD  - Biotech & Biomed Research Institute, Northwest University Taibai North Road 229 
      Xi'an Shaanxi 710069 China.
LA  - eng
PT  - Journal Article
DEP - 20200610
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9054505
COIS- The authors declare no conflict of interest.
EDAT- 2020/06/10 00:00
MHDA- 2020/06/10 00:01
PMCR- 2020/06/10
CRDT- 2022/05/06 05:43
PHST- 2020/04/17 00:00 [received]
PHST- 2020/06/02 00:00 [accepted]
PHST- 2022/05/06 05:43 [entrez]
PHST- 2020/06/10 00:00 [pubmed]
PHST- 2020/06/10 00:01 [medline]
PHST- 2020/06/10 00:00 [pmc-release]
AID - d0ra03423a [pii]
AID - 10.1039/d0ra03423a [doi]
PST - epublish
SO  - RSC Adv. 2020 Jun 10;10(37):22234-22241. doi: 10.1039/d0ra03423a. eCollection 
      2020 Jun 8.