PMID- 35517729 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220507 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) VI - 10 IP - 35 DP - 2020 May 27 TI - Microbial-induced calcium carbonate precipitation: an experimental toolbox for in situ and real time investigation of micro-scale pH evolution. PG - 20485-20493 LID - 10.1039/d0ra03897k [doi] AB - Concrete is the second most consumed product by humans, after water. However, the production of conventional concrete causes more than 5% of anthropogenic CO(2) emissions and therefore there is a need for emission-reduced construction materials. One method to produce a solid, concrete-like construction material is microbial-induced calcium carbonate precipitation (MICP). To get a better understanding of MICP it is important to be able to follow local pH changes in dissolution and precipitation processes of CaCO(3). In this work we present a new method to study processes of MICP at the micro-scale in situ and in real time. We present two different methods to monitor the pH changes during the precipitation process of CaCO(3). In the first method, the average pH of small sample volumes is measured in real time, and pH changes are subsequently correlated with processes in the sample by comparing to optical microscope results. The second method is introduced to follow local pH changes at a grain scale in situ and in real time. Furthermore, local pH changes during the dissolution of CaCO(3) crystals are monitored. We demonstrate that these two methods are powerful tools to investigate the pH changes for both MICP precipitation and CaCO(3) dissolution for knowledge-based improvement of MICP-based material properties. CI - This journal is (c) The Royal Society of Chemistry. FAU - Zehner, Jennifer AU - Zehner J AUID- ORCID: 0000-0002-5660-022X AD - Department of Physics, Norwegian University of Science and Technology (NTNU) Trondheim Norway jennifer.s.zehner@ntnu.no pawel.sikorski@ntnu.no. FAU - Royne, Anja AU - Royne A AUID- ORCID: 0000-0002-6868-6251 AD - The Njord Centre, Department of Physics, University of Oslo (UiO) Oslo Norway. FAU - Wentzel, Alexander AU - Wentzel A AD - Department of Biotechnology and Nanomedicine, SINTEF Industry Trondheim Norway. FAU - Sikorski, Pawel AU - Sikorski P AUID- ORCID: 0000-0001-9413-1623 AD - Department of Physics, Norwegian University of Science and Technology (NTNU) Trondheim Norway jennifer.s.zehner@ntnu.no pawel.sikorski@ntnu.no. LA - eng PT - Journal Article DEP - 20200528 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC9054232 COIS- There are no conflicts to declare. EDAT- 2020/05/28 00:00 MHDA- 2020/05/28 00:01 PMCR- 2020/05/28 CRDT- 2022/05/06 05:51 PHST- 2020/04/30 00:00 [received] PHST- 2020/05/20 00:00 [accepted] PHST- 2022/05/06 05:51 [entrez] PHST- 2020/05/28 00:00 [pubmed] PHST- 2020/05/28 00:01 [medline] PHST- 2020/05/28 00:00 [pmc-release] AID - d0ra03897k [pii] AID - 10.1039/d0ra03897k [doi] PST - epublish SO - RSC Adv. 2020 May 28;10(35):20485-20493. doi: 10.1039/d0ra03897k. eCollection 2020 May 27.