PMID- 35519172
OWN - NLM
STAT- MEDLINE
DCOM- 20220509
LR  - 20220716
IS  - 1937-8688 (Electronic)
VI  - 41
DP  - 2022
TI  - Association between human leukocyte antigen class II (HLA-DRB and -DQB) alleles 
      and outcome of exposure to Mycobacterium tuberculosis: a cross-sectional study in 
      Nairobi, Kenya.
PG  - 149
LID - 10.11604/pamj.2022.41.149.30056 [doi]
LID - 149
AB  - INTRODUCTION: human leukocyte antigen (HLA) class II alleles play an important 
      role in the early immune response to tuberculosis (TB) by presenting antigenic 
      peptides to CD4+ T cells, hence polymorphisms in those genes can influence the 
      efficiency of the immune response to infection and progression to active disease. 
      METHODS: an analytical cross-sectional study of adult pulmonary tuberculosis 
      (PTB) patients at Mbagathi County Hospital, Nairobi and their HHCs. 
      Sociodemographic data were captured on questionnaires and clinical data extracted 
      from patient files. Intravenous blood samples were drawn for interferon-gamma 
      release assay (IGRA) to determine latent tuberculosis infection (LTBI) among 
      HHCs, and for extraction of DNA used in typing of HLA-DQB1 and HLA-DRB1 alleles 
      by PCR sequence specific primer amplification. Chi-square and Fisher's exact test 
      were used to compare the HLA type II allele frequencies of LTBI negative HHCs, 
      LTBI positive HHCs and active TB patients. Logistic regression was used to adjust 
      for HIV status. RESULTS: the HLA-DQB1 and HLA-DRB1 alleles were analyzed in 17 
      PTB and 37 HHCs. Nineteen (19) HHCs were LTBI positive, while 18 were LTBI 
      negative. The frequency of DRB3*1 was 0.17-fold lower [95% CI=0.03-0.83] among 
      PTB patients compared to HHCs before adjustment for HIV status (p=0.048). The 
      frequency of the DRB5*2 allele was significantly higher (p=0.013) among PTB 
      patients (23.5%) compared to HHCS (0.00%). After adjusting for HIV status, the 
      frequency of DRB1*14 was 12-fold higher [95% CI=1.11-138.2] among PTB patients 
      compared to HHCs (p=0.040). CONCLUSION: the higher frequencies of HLA-DRB5*2 and 
      HLA-DRB1*14 alleles in PTB patients suggest a likely association with progression 
      to active PTB. The higher frequency of HLA-DRB3*1 allele among LTBI negative HHCs 
      shows its likely protective role against M. tuberculosis infection in this 
      population.
CI  - Copyright: Susan Odera et al.
FAU - Odera, Susan
AU  - Odera S
AD  - Department of Medical Microbiology and Immunology, University of Nairobi, 
      Nairobi, Kenya.
FAU - Mureithi, Marianne
AU  - Mureithi M
AD  - Department of Medical Microbiology and Immunology, University of Nairobi, 
      Nairobi, Kenya.
AD  - Kenya Aids Vaccine Initiative (KAVI) Institute of Clinical Research, University 
      of Nairobi, Nairobi, Kenya.
FAU - Aballa, Andrew
AU  - Aballa A
AD  - Department of Medical Laboratory Sciences, School of Medicine, Kenyatta 
      University, Nairobi, Kenya.
FAU - Onyango, Noel
AU  - Onyango N
AD  - Department of Clinical Medicine and Therapeutics, School of Medicine, University 
      of Nairobi, Nairobi, Kenya.
FAU - Kazungu, Samwel
AU  - Kazungu S
AD  - Kenya Aids Vaccine Initiative (KAVI) Institute of Clinical Research, University 
      of Nairobi, Nairobi, Kenya.
FAU - Ogolla, Simon
AU  - Ogolla S
AD  - Kenya Aids Vaccine Initiative (KAVI) Institute of Clinical Research, University 
      of Nairobi, Nairobi, Kenya.
FAU - Kaiyare, George
AU  - Kaiyare G
AD  - Biozeq Kenya Molecular Laboratory, Nairobi, Kenya.
FAU - Anzala, Omu
AU  - Anzala O
AD  - Department of Medical Microbiology and Immunology, University of Nairobi, 
      Nairobi, Kenya.
AD  - Kenya Aids Vaccine Initiative (KAVI) Institute of Clinical Research, University 
      of Nairobi, Nairobi, Kenya.
FAU - Oyugi, Julius
AU  - Oyugi J
AD  - Department of Medical Microbiology and Immunology, University of Nairobi, 
      Nairobi, Kenya.
AD  - Institute of Tropical and Infectious Diseases, University of Nairobi, Nairobi, 
      Kenya.
LA  - eng
PT  - Journal Article
DEP - 20220221
PL  - Uganda
TA  - Pan Afr Med J
JT  - The Pan African medical journal
JID - 101517926
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Cross-Sectional Studies
MH  - Gene Frequency
MH  - *HIV Infections
MH  - HLA-DRB1 Chains/genetics
MH  - Humans
MH  - Kenya
MH  - *Latent Tuberculosis/epidemiology/genetics
MH  - *Mycobacterium tuberculosis/genetics
MH  - *Tuberculosis, Lymph Node
MH  - *Tuberculosis, Pulmonary/epidemiology/genetics
PMC - PMC9046861
OTO - NOTNLM
OT  - Human leucocyte antigen
OT  - Kenya
OT  - household contacts
OT  - latent tuberculosis
OT  - pulmonary tuberculosis
COIS- The authors declare no competing interest.
EDAT- 2022/05/07 06:00
MHDA- 2022/05/10 06:00
PMCR- 2022/02/21
CRDT- 2022/05/06 06:03
PHST- 2021/05/28 00:00 [received]
PHST- 2022/02/02 00:00 [accepted]
PHST- 2022/05/06 06:03 [entrez]
PHST- 2022/05/07 06:00 [pubmed]
PHST- 2022/05/10 06:00 [medline]
PHST- 2022/02/21 00:00 [pmc-release]
AID - PAMJ-41-149 [pii]
AID - 10.11604/pamj.2022.41.149.30056 [doi]
PST - epublish
SO  - Pan Afr Med J. 2022 Feb 21;41:149. doi: 10.11604/pamj.2022.41.149.30056. 
      eCollection 2022.