PMID- 35523281 OWN - NLM STAT- MEDLINE DCOM- 20220620 LR - 20220808 IS - 1096-0953 (Electronic) IS - 0013-9351 (Linking) VI - 212 IP - Pt C DP - 2022 Sep TI - Precipitation-based microscale enzyme reactors coupled with porous and adhesive elastomer for effective bacterial decontamination and membrane antifouling on-demand. PG - 113407 LID - S0013-9351(22)00734-4 [pii] LID - 10.1016/j.envres.2022.113407 [doi] AB - Bacterial contamination of water environments can cause various troubles in various areas. As one of potential solutions, we develop enzyme-immobilized elastomer, and demonstrate the uses of enzyme reactions on-demand for effective microbial decontamination and antifouling. Asymmetrically-structured elastomer is prepared by combining two polydimethylsiloxane (PDMS) layers with different degrees of crosslinking: highly-crosslinked and lightly-crosslinked PDMS layers. At the surface of highly-crosslinked PDMS layer, porous structure with average diameter of 842 nm is formed by dissolving pre-packed and entrapped latex beads. Lightly-crosslinked PDMS on the other side, due to its adhesive nature, enables iterative attachments on various materials under either dry or wet condition. Glucose oxidase (GOx) is immobilized by using the pores at the surface of highly-crosslinked PDMS matrix via a ship-in-a-bottle protocol of precipitation-based microscale enzyme reactor (p-MER), which consists of GOx adsorption, precipitation and chemical crosslinking (EAPC). As a result, crosslinked enzyme aggregates (CLEAs) of GOx not only are well entrapped within many pores of highly-crosslinked PDMS layer (ship-in-bottle) but also cover the external surface of matrix, both of which are well connected together. Highly-interconnected network of CLEAs themselves effectively prevents enzyme leaching, which shows the 25% residual activity of GOx under shaking at 200 rpm for 156 days after 48% initial drop of loosely-bound p-MER after 4 days. In presence of glucose, the underwater attachment of biocatalytic elastomer demonstrates the generation of hydrogen peroxide via p-MER-catalyzed glucose oxidation, exhibiting effective biocidal activities against both gram-positive S. aureus and gram-negative E. coli. Adhesion-induced GOx-catalyzed reaction also alleviates the biofouling of membrane, suggesting its extendibility to various engineering systems being suffered by biofouling. This study of biocatalytic elastomer has demonstrated its new opportunities for the facile and on-demand enzyme-catalyzed reactions in various environmental applications, such as bactericidal treatment, water treatment/purification, and pollutant degradation. CI - Copyright (c) 2022 Elsevier Inc. All rights reserved. FAU - Yoon, YoungChul AU - Yoon Y AD - Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea. FAU - Kim, Han Sol AU - Kim HS AD - Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea. FAU - Yoon, Seji AU - Yoon S AD - Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea. FAU - Yeon, Kyung-Min AU - Yeon KM AD - Engineering Center, Samsung C&T Corporation, Tower B, 26, Sangil-ro, 6- gil, Gangdong-gu, Seoul, Republic of Korea. Electronic address: yeonkmin@naver.com. FAU - Kim, Jungbae AU - Kim J AD - Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea. Electronic address: jbkim3@korea.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220504 PL - Netherlands TA - Environ Res JT - Environmental research JID - 0147621 RN - 0 (Adhesives) RN - 0 (Elastomers) RN - 0 (Enzymes, Immobilized) RN - EC 1.1.3.4 (Glucose Oxidase) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Adhesives MH - *Biofouling/prevention & control MH - Decontamination MH - Elastomers MH - Enzymes, Immobilized/chemistry/metabolism MH - Escherichia coli MH - Glucose MH - Glucose Oxidase/chemistry/metabolism MH - Porosity MH - Staphylococcus aureus/metabolism OTO - NOTNLM OT - Bacterial decontamination OT - Biocatalytic elastomer OT - Glucose oxidase OT - Membrane antifouling OT - Polydimethylsiloxane OT - Precipitation-based microscale enzyme reactor EDAT- 2022/05/07 06:00 MHDA- 2022/06/22 06:00 CRDT- 2022/05/06 19:22 PHST- 2022/01/28 00:00 [received] PHST- 2022/04/26 00:00 [revised] PHST- 2022/04/28 00:00 [accepted] PHST- 2022/05/07 06:00 [pubmed] PHST- 2022/06/22 06:00 [medline] PHST- 2022/05/06 19:22 [entrez] AID - S0013-9351(22)00734-4 [pii] AID - 10.1016/j.envres.2022.113407 [doi] PST - ppublish SO - Environ Res. 2022 Sep;212(Pt C):113407. doi: 10.1016/j.envres.2022.113407. Epub 2022 May 4.