PMID- 3552814
OWN - NLM
STAT- MEDLINE
DCOM- 19870529
LR  - 20190816
IS  - 0012-186X (Print)
IS  - 0012-186X (Linking)
VI  - 29
IP  - 12
DP  - 1986 Dec
TI  - Polyamine-enhanced casein kinase II in mouse pancreatic islets.
PG  - 888-92
AB  - The occurrence of polyamine-stimulated protein kinase (casein kinase II) in 
      cytosol of mouse pancreatic islets was investigated. Islet protein 
      phosphorylation was enhanced by spermidine, spermine, lysine-rich histone and 
      polylysine; the major endogenous substrates in the cytosol were three proteins of 
      Mr 50,000, 55,000 and 100,000. Cadaverine and putrescine were without effects. A 
      Mr 100,000 protein is a major substrate for Ca2+-calmodulin-dependent protein 
      kinase, and Mr 50,000 and 55,000 proteins are substrates for cyclic adenosine 
      3',5'-cyclic monophosphate (AMP) dependent protein kinase in mouse islets. 
      However, neither cyclic-AMP-dependent protein kinase inhibitor nor 
      trifluoperazine inhibited polyamine-enhanced protein phosphorylation. Both basal 
      and polyamine-enhanced protein phosphorylation patterns were identical when 
      either [gamma-32P] adenosine 5'-triphosphate (ATP) or [gamma-32P] guanosine 
      5'-triphosphate (GTP) was used as phosphate donors, indicative of the presence of 
      a polyamine-stimulated casein kinase II in pancreatic islets. It is suggested 
      that polyamines and polyamine-enhanced casein kinase II activity may have an 
      important role in regulation of protein phosphorylation in pancreatic islets.
FAU - Thams, P
AU  - Thams P
FAU - Capito, K
AU  - Capito K
FAU - Hedeskov, C J
AU  - Hedeskov CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Diabetologia
JT  - Diabetologia
JID - 0006777
RN  - 0 (Histones)
RN  - 0 (Polyamines)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 214IZI85K3 (Trifluoperazine)
RN  - 25104-18-1 (Polylysine)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Casein Kinases)
RN  - L90BEN6OLL (Cadaverine)
RN  - U87FK77H25 (Spermidine)
RN  - V10TVZ52E4 (Putrescine)
SB  - IM
MH  - Animals
MH  - Cadaverine/pharmacology
MH  - Casein Kinases
MH  - Histones/pharmacology
MH  - In Vitro Techniques
MH  - Islets of Langerhans/drug effects/*enzymology
MH  - Male
MH  - Mice
MH  - Phosphorylation
MH  - Polyamines/*pharmacology
MH  - Polylysine/pharmacology
MH  - Protein Kinase Inhibitors
MH  - Protein Kinases/*metabolism
MH  - Putrescine/pharmacology
MH  - Spermidine/pharmacology
MH  - Spermine/pharmacology
MH  - Trifluoperazine/pharmacology
EDAT- 1986/12/01 00:00
MHDA- 1986/12/01 00:01
CRDT- 1986/12/01 00:00
PHST- 1986/12/01 00:00 [pubmed]
PHST- 1986/12/01 00:01 [medline]
PHST- 1986/12/01 00:00 [entrez]
AID - 10.1007/BF00870145 [doi]
PST - ppublish
SO  - Diabetologia. 1986 Dec;29(12):888-92. doi: 10.1007/BF00870145.