PMID- 35528279
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 17
IP  - 1
DP  - 2022
TI  - Study on the mechanism of inhibiting patulin production by fengycin.
PG  - 372-379
LID - 10.1515/biol-2022-0041 [doi]
AB  - Penicillium expansum is the main cause of apple rot. Besides, it can also produce 
      mycotoxin patulin (PAT). Therefore, the search for substances that can inhibit 
      the activity and toxigenicity of P. expansum has become a hot research topic. 
      This study investigates the inhibitory effects of fengycin on patulin production 
      in P. expansum. P. expansum was cultured under different environments with 
      different concentrations of fengycin. The patulin content produced per unit 
      weight of P. expansum mycelium was detected and determined by high pressure 
      liquid chromatography (HPLC). Synergy brands (SYBR) GreenI Real-time PCR was used 
      to detect the expression levels of 6-methylsalicylic acid synthase (6-MSAS) and 
      isoepoxydon dehydrogenase (IDH), which were the key genes of producing patulin of 
      P. expansum mycelium, in the conditions treated by fengycin and untreated. After 
      fengycin treatments, not only the patulin content in every unit weight of P. 
      expansum mycelium but also the expression level of 6-MSAS decreased 
      significantly. The expression level of 6-MSAS of treatment was 0.11 folds of 
      control. However, the expression level of IDH treated by fengycin decreased 
      slightly. Fengycin could inhibit the P. expansum from producing patulin by 
      downregulating the expression of key synthetic genes 6-MSAS.
CI  - (c) 2022 Ruimin Fu et al., published by De Gruyter.
FAU - Fu, Ruimin
AU  - Fu R
AD  - College of Health Management, Henan Finance University, Zhengzhou, Henan, China.
AD  - College of Life Science, Shaanxi Normal University, Xi'an, Shaanxi, China.
FAU - Tang, Wei
AU  - Tang W
AD  - College of Health Management, Henan Finance University, Zhengzhou, Henan, China.
FAU - Zhang, Hong
AU  - Zhang H
AD  - College of Health Management, Henan Finance University, Zhengzhou, Henan, China.
FAU - Zhang, Yulian
AU  - Zhang Y
AD  - College of Health Management, Henan Finance University, Zhengzhou, Henan, China.
FAU - Wang, Ding
AU  - Wang D
AD  - College of Health Management, Henan Finance University, Zhengzhou, Henan, China.
FAU - Chen, Wuling
AU  - Chen W
AD  - College of Life Science, Shaanxi Normal University, Xi'an, Shaanxi, China.
LA  - eng
PT  - Journal Article
DEP - 20220419
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC9019426
OTO - NOTNLM
OT  - 6-MSAS
OT  - IDH
OT  - Penicillium expansum
OT  - fengycin
OT  - patulin
COIS- Conflict of interest: Authors state no conflict of interest.
EDAT- 2022/05/10 06:00
MHDA- 2022/05/10 06:01
PMCR- 2022/04/19
CRDT- 2022/05/09 03:54
PHST- 2021/07/09 00:00 [received]
PHST- 2021/12/02 00:00 [revised]
PHST- 2022/01/03 00:00 [accepted]
PHST- 2022/05/09 03:54 [entrez]
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/05/10 06:01 [medline]
PHST- 2022/04/19 00:00 [pmc-release]
AID - biol-2022-0041 [pii]
AID - 10.1515/biol-2022-0041 [doi]
PST - epublish
SO  - Open Life Sci. 2022 Apr 19;17(1):372-379. doi: 10.1515/biol-2022-0041. 
      eCollection 2022.