PMID- 35528509
OWN - NLM
STAT- MEDLINE
DCOM- 20220510
LR  - 20220716
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - Intervertebral Disc Degeneration and Low Back Pain Depends on Duration and 
      Magnitude of Axial Compression.
PG  - 1045999
LID - 10.1155/2022/1045999 [doi]
LID - 1045999
AB  - PURPOSE: The pathological role of axial stress in intervertebral disc 
      degeneration (IDD) is controversial, and there was no quantified study until now. 
      Here, we tried to clarify the correlation between IDD or low back pain (LBP) and 
      axial stress at different duration and magnitude in vitro and in vivo. METHOD: In 
      vitro, the gene expression of aggrecan, matrix metalloproteinase-3 (MMP3), 
      calcitonin gene-related peptide (CGRP), and substance P (SP) was measured when 
      nucleus pulposus cells (NPCs) were compressed under gradual severity. In vivo, a 
      measurable Ilizarov-type compression apparatus was established for single 
      coccygeal (Co) intervertebral disc (IVD) compression of Co7-8 in mouse. Gradient 
      stress was placed at 0.4 Mpa (mild), 0.8 Mpa (moderate), and 1.2 Mpa (severe) for 
      three days to investigate the effect of the magnitude of axial stress. 
      Additionally, mild compression with 3, 7, and 14 days was used to determine the 
      effect of the duration of axial stress. Subsequently, we evaluated the severity 
      of IDD and LBP by radiological X-ray film; histological examination with H&E 
      staining; immunohistochemical analysis with collagen II, aggrecan, and CGRP 
      staining; and western blot analysis with collagen II, aggrecan, MMP-3, and 
      interleukin-1beta (IL-1beta). RESULTS: When NPCs suffered gradual increased mechanical 
      stress, the cells exhibited gradual downregulated expression of extracellular 
      matrix (ECM)-related gene of aggrecan, upregulated expression of IDD-related gene 
      of MMP3, and LBP-related gene of CGRP and SP. In the meantime, with different 
      magnitudes of axial stress, the IVD showed progressively severe IDD and LBP, with 
      gradual narrowing intervertebral height, destruction of IVD anatomy, decreased 
      ECM, and increased catabolic factors and proalgesic peptides. CONCLUSION: Axial 
      compression is one of the critical pathological factors to cause IDD and LBP, and 
      there was a strong positive correlation depended on the duration and magnitude of 
      compression.
CI  - Copyright (c) 2022 Jitian Li et al.
FAU - Li, Jitian
AU  - Li J
AUID- ORCID: 0000-0001-5449-8084
AD  - Henan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital), Henan 
      Provincial Orthopedic Institute, Zhengzhou 450000, China.
AD  - Henan University of Chinese Medicine, Zhengzhou 450046, China.
FAU - Ma, Yan
AU  - Ma Y
AD  - Henan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital), Henan 
      Provincial Orthopedic Institute, Zhengzhou 450000, China.
AD  - Henan University of Chinese Medicine, Zhengzhou 450046, China.
FAU - Jiao, Yucheng
AU  - Jiao Y
AD  - Department of Orthopeadics, Ruijin Hospital, Shanghai Jiaotong University School 
      of Medicine, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 
      Shanghai 200025, China.
FAU - Xu, Libo
AU  - Xu L
AD  - Department of Orthopeadics, Ruijin Hospital, Shanghai Jiaotong University School 
      of Medicine, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 
      Shanghai 200025, China.
FAU - Luo, Yage
AU  - Luo Y
AD  - Henan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital), Henan 
      Provincial Orthopedic Institute, Zhengzhou 450000, China.
AD  - Henan University of Chinese Medicine, Zhengzhou 450046, China.
FAU - Zheng, Jiancheng
AU  - Zheng J
AUID- ORCID: 0000-0002-8932-1876
AD  - Department of Orthopeadics, Ruijin Hospital, Shanghai Jiaotong University School 
      of Medicine, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 
      Shanghai 200025, China.
FAU - Zhang, Xingkai
AU  - Zhang X
AUID- ORCID: 0000-0002-0621-2499
AD  - Department of Orthopeadics, Ruijin Hospital, Shanghai Jiaotong University School 
      of Medicine, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 
      Shanghai 200025, China.
FAU - Chen, Zhe
AU  - Chen Z
AUID- ORCID: 0000-0002-9721-5301
AD  - Department of Orthopeadics, Ruijin Hospital, Shanghai Jiaotong University School 
      of Medicine, Shanghai 200025, China.
AD  - Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases 
      with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and 
      Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 
      Shanghai 200025, China.
LA  - eng
PT  - Journal Article
DEP - 20220429
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Aggrecans)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Aggrecans/genetics
MH  - Animals
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Collagen/metabolism
MH  - *Intervertebral Disc/metabolism
MH  - *Intervertebral Disc Degeneration/pathology
MH  - *Low Back Pain
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Mice
PMC - PMC9076309
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/05/10 06:00
MHDA- 2022/05/11 06:00
PMCR- 2022/04/29
CRDT- 2022/05/09 03:57
PHST- 2022/02/04 00:00 [received]
PHST- 2022/03/15 00:00 [revised]
PHST- 2022/04/09 00:00 [accepted]
PHST- 2022/05/09 03:57 [entrez]
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/05/11 06:00 [medline]
PHST- 2022/04/29 00:00 [pmc-release]
AID - 10.1155/2022/1045999 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 Apr 29;2022:1045999. doi: 10.1155/2022/1045999. 
      eCollection 2022.