PMID- 35528565 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231102 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) VI - 9 IP - 46 DP - 2019 Aug 23 TI - Epigallocatechin gallate prevents senescence by alleviating oxidative stress and inflammation in WI-38 human embryonic fibroblasts. PG - 26787-26798 LID - 10.1039/c9ra03313k [doi] AB - Increased levels of oxidative stress and inflammation are the underlying mechanisms behind the aging process and age-related diseases. The purpose of our research is to explore whether epigallocatechin gallate (EGCG) can extend replicative life span by preventing the oxidative stress and inflammatory effects of WI-38 fibroblasts and the involved mechanisms in vitro. WI-38 cells were treated with different concentrations of EGCG (0, 25, 50 and 100 muM) at population doubling (PD) 25. At late-stage cells, we determined the age-associated genes with signaling through transcriptome sequencing. The expression profile of the targets in WI-38 fibroblasts was confirmed by bioinformatics analysis, qPCR and western blot. We found that EGCG markedly decreased reactive oxygen species (ROS), and inflammation factors, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, and significantly increased cell proliferation at PD 35 and 45. EGCG treatments significantly decreased p53 and retinoblastoma (Rb) expressions, markedly increased p-Rb and E2F2 expressions as well as antioxidant enzymes and superoxide dismutase (SOD) 1 and SOD2 content, and obviously decreased the expressions of inflammation factors IL-32, TNF-alpha expressions at PD 45 WI-38 cells. Moreover, the effects were changed by EGCG treatment by p53 siRNA or overexpression. These findings in our studies reveal that EGCG treatments improved senescence and enhanced the replicative life span through alleviating oxidative stress and inflammation in WI-38 fibroblasts. CI - This journal is (c) The Royal Society of Chemistry. FAU - Zhang, Qiao AU - Zhang Q AD - Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University Harbin 150086 China qq949495028@163.com 15645619696@163.com 18846753715@163.com lingfanplus@163.com niuyucun@163.com liying_helen@163.com +86 451 87502885 +86 451 87508731. AD - Department of Public Health College, Kunming Medical University Kunming 650550 China. FAU - Wu, Yuqing AU - Wu Y AD - Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University Harbin 150086 China qq949495028@163.com 15645619696@163.com 18846753715@163.com lingfanplus@163.com niuyucun@163.com liying_helen@163.com +86 451 87502885 +86 451 87508731. FAU - Guan, Yue AU - Guan Y AD - Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University Harbin 150086 China qq949495028@163.com 15645619696@163.com 18846753715@163.com lingfanplus@163.com niuyucun@163.com liying_helen@163.com +86 451 87502885 +86 451 87508731. FAU - Ling, Fan AU - Ling F AD - Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University Harbin 150086 China qq949495028@163.com 15645619696@163.com 18846753715@163.com lingfanplus@163.com niuyucun@163.com liying_helen@163.com +86 451 87502885 +86 451 87508731. FAU - Li, Ying AU - Li Y AD - Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University Harbin 150086 China qq949495028@163.com 15645619696@163.com 18846753715@163.com lingfanplus@163.com niuyucun@163.com liying_helen@163.com +86 451 87502885 +86 451 87508731. FAU - Niu, Yucun AU - Niu Y AUID- ORCID: 0000-0002-4879-6664 AD - Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University Harbin 150086 China qq949495028@163.com 15645619696@163.com 18846753715@163.com lingfanplus@163.com niuyucun@163.com liying_helen@163.com +86 451 87502885 +86 451 87508731. LA - eng PT - Journal Article DEP - 20190827 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC9070540 COIS- The authors declare that they have no conflict of interests. EDAT- 2019/08/27 00:00 MHDA- 2019/08/27 00:01 PMCR- 2019/08/27 CRDT- 2022/05/09 03:57 PHST- 2019/05/04 00:00 [received] PHST- 2019/08/08 00:00 [accepted] PHST- 2022/05/09 03:57 [entrez] PHST- 2019/08/27 00:00 [pubmed] PHST- 2019/08/27 00:01 [medline] PHST- 2019/08/27 00:00 [pmc-release] AID - c9ra03313k [pii] AID - 10.1039/c9ra03313k [doi] PST - epublish SO - RSC Adv. 2019 Aug 27;9(46):26787-26798. doi: 10.1039/c9ra03313k. eCollection 2019 Aug 23.