PMID- 35529931 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220716 IS - 1741-427X (Print) IS - 1741-4288 (Electronic) IS - 1741-427X (Linking) VI - 2022 DP - 2022 TI - Comparative Pharmacokinetics of Seven Major Compounds in Normal and Atherosclerosis Mice after Oral Administration of Simiao Yong'an Decoction. PG - 4604601 LID - 10.1155/2022/4604601 [doi] LID - 4604601 AB - Simiao Yong'an decoction (SMYAD), a classic traditional Chinese medicine formula, has been used to treat atherosclerosis (AS) in clinical in China, but its therapeutic mechanism and pharmacodynamic material basis are not clear. In this study, the AS model was caused by a high-fat diet and perivascular carotid collar placement (PCCP), and SMYAD was orally administered to the model and normal mice. A rapid, sensitive, selective, and reliable method using ultrahigh-performance liquid chromatography (UHPLC) system combined with a Q Exactive HF-X mass spectrometer (UHPLC-Q Exactive HF-X MS) was established and validated for the simultaneous determination of seven compounds, including harpagide, chlorogenic acid, swertiamarin, sweroside, angoroside C, liquiritin, and isoliquiritigenin in the plasma of normal and AS mice. The specificity, linearity, precision, accuracy, recovery, and stability of the method were all within the acceptable criteria. The results showed that some pharmacokinetic behaviors of harpagide, chlorogenic acid, and isoliquiritigenin were significantly different among the two groups of mice. The specific parameter changes were harpagide (AUC(0-t) and AUC(0-infinity) were 11075.09 +/- 2132.38 and 16221.95 +/- 5622.42 ng.mL(-1).h, respectively; CLz/F was 2.45 +/- 0.87 L/h/mg), chlorogenic acid (t (1/2) was 21.59 +/- 9.16 h; AUC(0-infinity) was 2637.51 +/- 322.54 ng.mL(-1).h; CLz/F was 13.49 +/- 1.81 L/h/mg) and isoliquiritigenin (AUC(0-t) and AUC(0-infinity) were 502.25 +/- 165.65 and 653.68 +/- 251.34 ng.mL(-1).h, respectively; CLz/F was 62.16 +/- 23.35 L/h/mg) were altered under the pathological status of AS. These differences might be partly ascribed to the changes in gastrointestinal microbiota, nonspecific drug transporters, and cytochrome P450 activity under the AS state, providing research ideas and experimental basis for pharmacological effects and pharmacodynamic material basis. CI - Copyright (c) 2022 Ke-han Sun et al. FAU - Sun, Ke-Han AU - Sun KH AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. AD - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Yang, Man-Fang AU - Yang MF AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. AD - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Xu, Xin-Rui AU - Xu XR AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Li, Yang AU - Li Y AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. AD - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Gao, Zhao AU - Gao Z AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Zhang, Qing-Yue AU - Zhang QY AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Li, Hui AU - Li H AD - School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Wang, Shu-Qi AU - Wang SQ AD - Zibo Hospital of Traditional Chinese Medicine, Zibo 255399, Shandong, China. FAU - Lou, Li-Xia AU - Lou LX AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Wu, Ai-Ming AU - Wu AM AUID- ORCID: 0000-0002-2746-5532 AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Jin, Qiu-Shuo AU - Jin QS AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Wu, Sheng-Xian AU - Wu SX AUID- ORCID: 0000-0002-6034-9728 AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. FAU - Nie, Bo AU - Nie B AUID- ORCID: 0000-0003-4522-8829 AD - Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. LA - eng PT - Journal Article DEP - 20220428 PL - United States TA - Evid Based Complement Alternat Med JT - Evidence-based complementary and alternative medicine : eCAM JID - 101215021 PMC - PMC9071858 COIS- The authors declare no conflicts of interest. EDAT- 2022/05/10 06:00 MHDA- 2022/05/10 06:01 PMCR- 2022/04/28 CRDT- 2022/05/09 04:17 PHST- 2021/12/31 00:00 [received] PHST- 2022/03/31 00:00 [revised] PHST- 2022/04/11 00:00 [accepted] PHST- 2022/05/09 04:17 [entrez] PHST- 2022/05/10 06:00 [pubmed] PHST- 2022/05/10 06:01 [medline] PHST- 2022/04/28 00:00 [pmc-release] AID - 10.1155/2022/4604601 [doi] PST - epublish SO - Evid Based Complement Alternat Med. 2022 Apr 28;2022:4604601. doi: 10.1155/2022/4604601. eCollection 2022.