PMID- 35531700
OWN - NLM
STAT- MEDLINE
DCOM- 20220510
LR  - 20220510
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 47
IP  - 9
DP  - 2022 May
TI  - [Effects of different extracts of Dendrobium officinale on rats with chronic 
      pharyngitis induced by pepper water combined with ammonia].
PG  - 2525-2532
LID - 10.19540/j.cnki.cjcmm.20211223.703 [doi]
AB  - Dendrobium officinale can serve as Chinese medicinal material effective in 
      nourishing yin, clearing heat, and producing fluid, and is used to treat throat 
      diseases, but its active substances and mechanism are not clear. To clarify the 
      active fraction and underlying mechanism of D. officinale against chronic 
      pharyngitis(CP), the present study induced a CP model in rats by pepper water 
      combined with low-concentration ammonia, and crude polysaccharides of D. 
      officinale(DOP), non-polysaccharides of D. officinale(DON), and total extract of 
      D. officinale(DOT)(0.33 g.kg~(-1), calculated according to the crude drug) were 
      administered by gavage for six weeks. The changes in oral secretions and 
      pharyngeal conditions of rats with CP were observed and rated. The hematological 
      indicators were determined by an automatic hematology analyzer. The serum levels 
      of pro-inflammatory factors, such as tumor necrosis factor-alpha(TNF-alpha), 
      interleukin 1beta(IL-1beta), and interleukin 6(IL-6), and T-lymphocyte cytokines, 
      including interferon gamma(IFN-gamma), interleukin 4(IL-4), interleukin 17(IL-17), and 
      transforming growth factor beta1(TGF-beta1) were detected by the enzyme-linked 
      immunosorbent assay(ELISA). The proportions of CD3~+, CD4~+, and CD8~+cells in 
      peripheral blood T lymphocyte subsets were determined by the flow cytometry. The 
      histomorphological changes of the pharynx were observed by hematoxylin-eosin(HE) 
      staining. The protein expression of nuclear factor-kappaB P65(NF-kappaB P65), 
      cyclooxygenase-2(COX-2), F4/80, and monocyte chemoattractant protein-1(MCP-1) in 
      the pharynx were detected by immunohistochemistry and Western blot. The results 
      showed that DOP and DON could significantly relieve pharyngeal lesions, reduce 
      white blood cells(WBC) and lymphocytes(LYMP), decrease the levels of 
      pro-inflammatory factors TNF-alpha, IL-6, and IL-1beta, and inhibit the protein 
      expression of NF-kappaB P65, COX-2, F4/80, and MCP-1 in the pharynx. DOP was superior 
      in reducing oral secretions and serum IL-17 level and inferior in increasing 
      CD4~+/CD8~+ratio to DON. It is suggested that both polysaccharides and 
      non-polysaccharides of D. officinale have anti-PC effects and the 
      anti-inflammatory mechanism may be related to the regulation of T lymphocyte 
      distribution and inhibition of the inflammatory signaling pathways mediated by 
      NF-kappaB P65. The anti-inflammatory effect of DOP may be related to the regulation 
      of Th17/Treg balance, while that of DON may be related to the regulation of the 
      Th/Tc ratio.
FAU - Ji, Wen-Na
AU  - Ji WN
AD  - Zhejiang Chinese Medical University Hangzhou 310053, China.
FAU - Yan, Mei-Qiu
AU  - Yan MQ
AD  - Zhejiang Chinese Medical University Hangzhou 310053, China.
FAU - Su, Jie
AU  - Su J
AD  - Zhejiang Chinese Medical University Hangzhou 310053, China.
FAU - Yu, Jing-Jing
AU  - Yu JJ
AD  - Zhejiang Chinese Medical University Hangzhou 310053, China.
FAU - Chen, Su-Hong
AU  - Chen SH
AD  - Zhejiang University of Technology Hangzhou 310014, China.
FAU - Lyu, Gui-Yuan
AU  - Lyu GY
AD  - Zhejiang Chinese Medical University Hangzhou 310053, China.
FAU - Chen, Jian-Zhen
AU  - Chen JZ
AD  - Zhejiang Chinese Medical University Hangzhou 310053, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 059QF0KO0R (Water)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Ammonia/therapeutic use
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - Cyclooxygenase 2
MH  - *Dendrobium/chemistry
MH  - Interleukin-17/therapeutic use
MH  - Interleukin-6
MH  - NF-kappa B/metabolism
MH  - *Pharyngitis/drug therapy
MH  - Plant Extracts/chemistry
MH  - Polysaccharides/pharmacology
MH  - Rats
MH  - Tumor Necrosis Factor-alpha
MH  - Water
OTO - NOTNLM
OT  - Dendrobium officinale
OT  - NF-kappaB P65
OT  - T lymphocytes
OT  - chronic pharyngitis
OT  - non-polysaccharides
OT  - polysaccharides
EDAT- 2022/05/10 06:00
MHDA- 2022/05/11 06:00
CRDT- 2022/05/09 05:02
PHST- 2022/05/09 05:02 [entrez]
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/05/11 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20211223.703 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2022 May;47(9):2525-2532. doi: 
      10.19540/j.cnki.cjcmm.20211223.703.