PMID- 35531887
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220716
IS  - 2150-5608 (Electronic)
IS  - 2150-5594 (Print)
IS  - 2150-5594 (Linking)
VI  - 13
IP  - 1
DP  - 2022 Dec
TI  - Guanylate-Binding protein 2b regulates the AMPK/mTOR/ULK1 signalling pathway to 
      induce autophagy during Mycobacterium bovis infection.
PG  - 875-889
LID - 10.1080/21505594.2022.2073024 [doi]
AB  - Autophagic isolation and degradation of intracellular pathogens are employed by 
      host cells as primary innate immune defense mechanisms to control intercellular 
      M. bovis infection. In this study, RNA-Seq technology was used to obtain the 
      total mRNA from bone marrow-derived macrophages (BMDMs) infected with M. bovis at 
      6 and 24 h after infection. One of the differential genes, GBP2b, was also 
      investigated. Analysis of the significant pathway involved in GBP2b-coexpressed 
      mRNA demonstrated that GBP2b was associated with autophagy and autophagy-related 
      mammalian target of rapamycin (mTOR) signaling and AMP-activated protein kinase 
      (AMPK) signaling. The results of in vivo and in vitro experiments showed 
      significant up-regulation of GBP2b during M. bovis infection. For in vitro 
      validation, small interfering RNA-GBP2b plasmids were transfected into BMDMs and 
      RAW264.7 cells lines to down-regulate the expression of GBP2b. The results showed 
      that the down-regulation of GBP2b impaired autophagy via the AMPK/mTOR/ULK1 
      pathway, thereby promoting the intracellular survival of M. bovis. Further 
      studies revealed that the activation of AMPK signaling was essential for the 
      regulation of autophagy during M. bovis infection. These findings expand the 
      understanding of how GBP2b regulates autophagy and suggest that GBP2b may be a 
      potential target for the treatment of diseases caused by M. bovis.
FAU - Yu, Youli
AU  - Yu Y
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Pan, Jialiang
AU  - Pan J
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Liu, Mengting
AU  - Liu M
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Jiang, Haiqin
AU  - Jiang H
AUID- ORCID: 0000-0002-4924-0465
AD  - Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Nanjing, China.
FAU - Xiong, Jingshu
AU  - Xiong J
AD  - Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Nanjing, China.
FAU - Tao, Lei
AU  - Tao L
AD  - Nanjing Institute for Food and Drug Control, Nanjing, China.
FAU - Xue, Feng
AU  - Xue F
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Tang, Fang
AU  - Tang F
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
FAU - Wang, Hongsheng
AU  - Wang H
AD  - Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Nanjing, China.
FAU - Dai, Jianjun
AU  - Dai J
AD  - China Pharmaceutical University, Nanjing, China.
LA  - eng
SI  - figshare/10.6084/m9.figshare.16696033v3
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virulence
JT  - Virulence
JID - 101531386
RN  - 0 (Carrier Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/genetics/metabolism
MH  - Autophagy/genetics
MH  - Carrier Proteins
MH  - *Mycobacterium bovis/genetics
MH  - RNA, Messenger
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
PMC - PMC9132469
OTO - NOTNLM
OT  - AMPK
OT  - GBP2b
OT  - Mycobacterium bovis
OT  - RNA-Seq
OT  - ULK1
OT  - autophagy
OT  - mTOR
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/05/10 06:00
MHDA- 2022/05/25 06:00
PMCR- 2022/05/21
CRDT- 2022/05/09 05:31
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/05/09 05:31 [entrez]
PHST- 2022/05/21 00:00 [pmc-release]
AID - 2073024 [pii]
AID - 10.1080/21505594.2022.2073024 [doi]
PST - ppublish
SO  - Virulence. 2022 Dec;13(1):875-889. doi: 10.1080/21505594.2022.2073024.