PMID- 35532365
OWN - NLM
STAT- MEDLINE
DCOM- 20220511
LR  - 20220511
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 47
IP  - 4
DP  - 2022 Apr 25
TI  - [Effect of mild moxibustion on vascular aging in senescence rats based on local 
      MCP-1/MMP-2/TGF-beta1 pro-inflammatory signal loop].
PG  - 290-7
LID - 10.13702/j.1000-0607.20210472 [doi]
AB  - OBJECTIVE: To observe the effect of mild moxibustion on monocyte chemotaxis 
      protein 1 (MCP-1)/matrix metalloproteinase 2 (MMP-2)/transforming growth factor 
      beta1 (TGF-beta1) pro-inflammatory signal loop in senile rats, so as to explore its 
      mechanisms underlying improving vascular aging (VA). METHODS: Twenty-four male VA 
      SD rats were randomized into senium (VA) control, medication and moxibustion 
      groups, and other 8 young SD rats (aged 2 months) were used as the young control 
      group. The VA model was established by intraperitoneal injection of D-galactose 
      (300 mg.kg(-1).d(-1)) once daily for 4 weeks, and verified by serum total 
      testosterone (TT) and free testosterone (FT) levels. For rats of the moxibustion 
      group, mild moxibustion was applied to bilateral "Shenshu"(BL23) and 
      "Guanyuan"(CV4) for 20 min, once a day, 5 days a week for 8 weeks. Rats of the 
      medication group were treated by intraperitoneal injection of testosterone 
      propionate (7 mg.kg(-1).[3 d](-1)) once daily for 8 weeks except weekends, and 
      rats of the senium control and young control groups treated by intraperitoneal 
      injection of the same dose of normal saline, once daily for 8 weeks except 
      weekends. The duration of exhausted swimming (DES) before and after the treatment 
      was recorded. H.E. staining and Masson staining were used to observe 
      histopathological changes and collagen fiber content of the thoracic aortic 
      tissue, respectively. The contents of serum TT, FT and angiotensin 2 (Ang Ⅱ) were 
      determined by ELISA. The immunoactivity of aortic MCP-1 was detected by 
      immunohistochemistry, and the expression levels of aortic MCP-1, MMP-2 and TGF-beta1 
      were detected by Western blot. RESULTS: Compared with the young control group, 
      the levels of DES, serum TT and FT contents were significantly decreased 
      (P<0.01), while those of serum AngⅡ and collagen fiber contents, aortic MCP-1 
      immunoactivity and MMP-2, TGF-beta1 and MCP-1 protein expression considerably 
      increased in the senium control group (P<0.01). After the interventions, the 
      decreased levels of DES, serum TT and FT contents and the increased levels of 
      serum AngⅡ, collagen fiber contents, aortic MCP-1 immunoactivity and MMP-2, 
      TGF-beta1 and MCP-1 protein expression were reversed in both medication and 
      moxibustion groups (P<0.01, P<0.05). The therapeutic effect of mild moxibustion 
      was significantly superior to that of medication in down-regulating the aortic 
      collagen fiber, serum AngⅡ contents and MCP-1 immunoactivity and protein 
      expression (P<0.05). H.E. staining showed thickened endometrium and disordered 
      arrangement of vascular smooth muscles of the aorta in the senium group, and 
      thinner endometrium and regular and ordered arrangement of aortic vascular smooth 
      muscles in both moxibustion and medication groups. CONCLUSION: Mild moxibustion 
      may improve vascular aging in senescence rats, which is possibly by suppressing 
      vascular MCP-1/MMP-2/TGF-beta1 pro-inflammatory signal loop.
FAU - Li, Yi
AU  - Li Y
AD  - College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 
      Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of 
      Metabolic Diseases, Chongqing 400016, China.
FAU - Li, Xue-Zhi
AU  - Li XZ
AD  - College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 
      Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of 
      Metabolic Diseases, Chongqing 400016, China.
FAU - Liao, Dong-Mei
AU  - Liao DM
AD  - College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 
      Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of 
      Metabolic Diseases, Chongqing 400016, China.
FAU - Pang, Fang
AU  - Pang F
AD  - College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 
      Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of 
      Metabolic Diseases, Chongqing 400016, China.
FAU - Yao, Tai-Wan
AU  - Yao TW
AD  - Department of Traditional Chinese Medicine, Chongqing General Hospital, Chongqing 
      400014.
FAU - He, Xin-Lu
AU  - He XL
AD  - College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 
      Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of 
      Metabolic Diseases, Chongqing 400016, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Transforming Growth Factor beta1)
RN  - 3XMK78S47O (Testosterone)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Collagen
MH  - Female
MH  - Male
MH  - Matrix Metalloproteinase 2/genetics
MH  - *Moxibustion
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testosterone
MH  - Transforming Growth Factor beta1/genetics
OTO - NOTNLM
OT  - Aging
OT  - Mild moxibustion
OT  - Testosterone
OT  - Vascular aging
EDAT- 2022/05/10 06:00
MHDA- 2022/05/12 06:00
CRDT- 2022/05/09 10:32
PHST- 2022/05/09 10:32 [entrez]
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/05/12 06:00 [medline]
AID - 10.13702/j.1000-0607.20210472 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2022 Apr 25;47(4):290-7. doi: 10.13702/j.1000-0607.20210472.