PMID- 35533251
OWN - NLM
STAT- MEDLINE
DCOM- 20220616
LR  - 20220707
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 123
IP  - 6
DP  - 2022 Jun
TI  - Wnt/beta-catenin signaling pathway is involved in early dopaminergic differentiation 
      of trabecular meshwork-derived mesenchymal stem cells.
PG  - 1120-1129
LID - 10.1002/jcb.30269 [doi]
AB  - Permanent degeneration and loss of dopaminergic (DA) neurons in substantia nigra 
      is the main cause of Parkinson's disease. Considering the therapeutic application 
      of stem cells in neurodegeneration, we sought to examine the neurogenic 
      differentiation potential of the newly introduced neural crest originated 
      mesenchymal stem cells (MSCs), namely, trabecular meshwork-derived mesenchymal 
      stem cells (TM-MSCs) compared to two other sources of MSCs, adipose 
      tissue-derived stem cells (ADSCs) and bone marrow-derived mesenchymal stem cells 
      (BM-MSCs). The three types of cells were therefore cultured in the presence and 
      absence of a neural induction medium followed by the analysis of their 
      differentiation potentials. Our results showed that TM-MSCs exhibited enhanced 
      neural morphologies as well as higher expressions of MAP2 as the general neuron 
      marker and Nurr-1 as an early DA marker compared to the adipose tissue-derived 
      mesenchymal stem cells (AD-MSCs) and bone marrow-derived stem cells (BMSCs). 
      Also, analysis of Nurr-1 immunostaining showed more intense Nurr-1 stained nuclei 
      in the neurally induced TM-MSCs compared to those in the AD-MSCs, BMSCs, and 
      noninduced control TM-MSCs. To examine if Wnt/beta-catenin pathway drives TM-MSCs 
      towards a DA fate, we treated them with the Wnt agonist (CHIR, 3 muM) and the Wnt 
      antagonist (IWP-2, 3 muM). Our results showed that the expressions of Nurr-1 and 
      MAP2, as well as the Wnt/beta-catenin target genes, c-Myc and Cyclin D1, were 
      significantly increased in the CHIR-treated TM-MSCs, but significantly reduced in 
      those treated with IWP-2. Altogether, we declare first a higher neural potency of 
      TM-MSCs compared to the more commonly used MSCs, BMSCs and ADSCs, and second that 
      Wnt/beta-catenin activation directs the neurally induced TM-MSCs towards a DA 
      fate.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Sahebdel, Faezeh
AU  - Sahebdel F
AUID- ORCID: 0000-0003-4451-089X
AD  - Developmental Biology Laboratory, Department of Animal Biology, School of 
      Biology, College of Science, University of Tehran, Tehran, Iran.
FAU - Parvaneh Tafreshi, Azita
AU  - Parvaneh Tafreshi A
AUID- ORCID: 0000-0003-1678-2863
AD  - Developmental Biology Laboratory, Department of Animal Biology, School of 
      Biology, College of Science, University of Tehran, Tehran, Iran.
AD  - Department of Medical Biotechnology, National Institute of Genetic Engineering 
      and Biotechnology, Tehran, Iran.
FAU - Arefian, Ehsan
AU  - Arefian E
AUID- ORCID: 0000-0002-0758-4710
AD  - Department of Microbiology, School of Biology, College of Science, University of 
      Tehran, Tehran, Iran.
FAU - Roussa, Eleni
AU  - Roussa E
AUID- ORCID: 0000-0002-0495-1597
AD  - Department of Molecular Embryology, Faculty of Medicine, Institute of Anatomy and 
      Cell Biology, Albert Ludwigs University Freiburg, Freiburg, Germany.
FAU - Nadri, Samad
AU  - Nadri S
AD  - Department of Medical Nanotechnology, School of Medicine, Zanjan University of 
      Medical Sciences, Zanjan, Iran.
FAU - Zeynali, Bahman
AU  - Zeynali B
AUID- ORCID: 0000-0001-6563-4969
AD  - Developmental Biology Laboratory, Department of Animal Biology, School of 
      Biology, College of Science, University of Tehran, Tehran, Iran.
LA  - eng
GR  - Iranian Vice Presidency for Science and Technology/
GR  - University of Tehran/
GR  - Iranian National Science Foundation/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220509
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (beta Catenin)
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Trabecular Meshwork/metabolism
MH  - *Wnt Signaling Pathway
MH  - beta Catenin/metabolism
OTO - NOTNLM
OT  - Wnt/beta-catenin signaling pathway
OT  - dopaminergic differentiation
OT  - trabecular meshwork mesenchymal stem cells
EDAT- 2022/05/10 06:00
MHDA- 2022/06/18 06:00
CRDT- 2022/05/09 14:35
PHST- 2022/04/20 00:00 [revised]
PHST- 2021/07/02 00:00 [received]
PHST- 2022/04/22 00:00 [accepted]
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/06/18 06:00 [medline]
PHST- 2022/05/09 14:35 [entrez]
AID - 10.1002/jcb.30269 [doi]
PST - ppublish
SO  - J Cell Biochem. 2022 Jun;123(6):1120-1129. doi: 10.1002/jcb.30269. Epub 2022 May 
      9.