PMID- 35533615
OWN - NLM
STAT- MEDLINE
DCOM- 20220615
LR  - 20220616
IS  - 2300-732X (Electronic)
IS  - 1642-431X (Linking)
VI  - 22
IP  - 2
DP  - 2022 Jun
TI  - Hsa_circ_0001495 contributes to cervical cancer progression by targeting 
      miR-526b-3p/TMBIM6/mTOR axis.
PG  - 100648
LID - S1642-431X(22)00047-X [pii]
LID - 10.1016/j.repbio.2022.100648 [doi]
AB  - Cervical cancer (CC) is a common gynecological malignant tumor, causing poor 
      survival rate. Circular RNAs (circRNAs) are abundantly expressed in CC with their 
      stable loop structure. However, the underlying mechanism and biological function 
      of circRNAs remained unclear. Using quantitative real-time polymerase chain 
      reaction (qRT-PCR) or western blot assay, we measured the expression of 
      hsa_circ_0001495, miR-526b-3p, and transmembrane Bax inhibitor motif containing 6 
      (TMBIM6) in CC tissues and cells. The relationship between miR-526b-3p and 
      hsa_circ_0001495 or TMBIM6 was investigated by bioinformatics analysis, 
      dual-luciferase and RIP analysis. Enzyme linked immunosorbent assay (ELISA) was 
      conducted to evaluate glucose consumption and lactate production. 
      5-ethynyl-2'-deoxyuridine (EDU) assay were used to test cell proliferation. Cell 
      apoptosis was analyzed by using flow cytometry assay. Transwell and wound-healing 
      assays were used to measure cell invasion and migration. The expression of 
      proteins was examined by western blot. Xenograft assay was applied to detect the 
      effect of hsa_circ_0001495 in vivo. Our finding showed that hsa_circ_0001495 and 
      TMBIM6 expression were upregulated, while miR-526b-3p was downregulated in CC 
      tissues and cell lines. Hsa_circ_0001495 knockdown or TMBIM6 knockdown suppressed 
      cell proliferation, migration, glycolysis, while promoted cell apoptosis in 
      vitro, and hsa_circ_0001495 silence curbed tumor growth in vivo. Beside, 
      hsa_circ_0001495 exerted its function in CC by positively regulating TMBIM6. 
      Furthermore, hsa_circ_0001495 acted as a sponge for miR-526b-3p to regulate 
      TMBIM6 expression. Hsa_circ_0001495/miR-526b-3p/TMBIM6 axis also regulated the 
      phosphorylation of mammalian target of rapamycin (mTOR) in CC cells. In summary, 
      hsa_circ_0001495 regulated the progression of CC by regulating 
      miR-526b-3p/TMBIM6/mTOR pathway.
CI  - Copyright (c) 2022 Society for Biology of Reproduction & the Institute of Animal 
      Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. 
      Published by Elsevier B.V. All rights reserved.
FAU - Zhang, Xiuzhi
AU  - Zhang X
AD  - Department of Gynecology, The Affiliated Xuzhou Municipal Hospital of Xuzhou 
      Medical University, Xuzhou 221000, Jiangsu, China.
FAU - Zheng, Xiaoli
AU  - Zheng X
AD  - Department of Gynecology, The Affiliated Xuzhou Municipal Hospital of Xuzhou 
      Medical University, Xuzhou 221000, Jiangsu, China. Electronic address: 
      zxl20180620@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220506
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (TMBIM6 protein, human)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Membrane Proteins/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Circular/genetics
MH  - Sirolimus
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - *Uterine Cervical Neoplasms/genetics
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Cervical cancer
OT  - Glycolysis
OT  - Hsa_circ_0001495
OT  - MiR-526b-3p
OT  - TMBIM6
EDAT- 2022/05/10 06:00
MHDA- 2022/06/16 06:00
CRDT- 2022/05/09 18:27
PHST- 2021/12/07 00:00 [received]
PHST- 2022/04/24 00:00 [revised]
PHST- 2022/04/25 00:00 [accepted]
PHST- 2022/05/10 06:00 [pubmed]
PHST- 2022/06/16 06:00 [medline]
PHST- 2022/05/09 18:27 [entrez]
AID - S1642-431X(22)00047-X [pii]
AID - 10.1016/j.repbio.2022.100648 [doi]
PST - ppublish
SO  - Reprod Biol. 2022 Jun;22(2):100648. doi: 10.1016/j.repbio.2022.100648. Epub 2022 
      May 6.