PMID- 35536069
OWN - NLM
STAT- MEDLINE
DCOM- 20220512
LR  - 20220512
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 68
IP  - 5
DP  - 2022 May 1
TI  - Serum Trimethylamine N-oxide and the Diversity of the Intestinal Microbial Flora 
      in Type 2 Diabetes Complicated by Diabetic Kidney Disease.
LID - 10.7754/Clin.Lab.2021.210836 [doi]
AB  - BACKGROUND: Trimethylamine N-oxide (TMAO) serves as a metabolite of intestinal 
      bacteria as well as a urotoxin influencing the prognosis of chronic kidney 
      disease (CKD), which has become a research hotspot in the field of kidney 
      disease. This study preliminarily explored the alternations of the microbial 
      flora and serum TMAO in patients with type 2 diabetes mellitus (T2DM) complicated 
      with diabetic kidney disease (DKD). METHODS: Seventeen T2DM patients at the 
      Affiliated Hospital of Zunyi Medical University between September 2018 and 
      February 2019 were included. Among these patients, 8 patients had T2DM 
      complicated with DKD. Eight healthy volunteers constituted the control group. 
      Fresh stool was collected for Illumina sequencing. Based on the sequencing 
      outcomes, the flora diversity and species differences were analyzed. Serum TMAO, 
      cystatin C, urinary albumin/urine creatinine ratios (ACRs), and routine 
      biochemical outcomes were also compared. RESULTS: The DKD group exhibited a 
      significantly higher TMAO level than the remaining groups. The high-TMAO group 
      had a significantly increased ACR level compared with the low-TMAO group. TMAO 
      positively correlated with the ACR. Compared with the control group, the DKD 
      group exhibited a decreased flora diversity. At the genus level, both the T2DM 
      group and the DKD group showed decreased numbers of Alloprevotella and 
      Megasphaera compared with the control group. The difference in Megasphaera 
      between the DKD group and the control group was significant. CONCLUSIONS: The 
      alternation of the intestinal microbial flora may participate in the development 
      of DKD, and TMAO and chronic inflammation might be important factors for DKD 
      development.
FAU - Yang, Mengxue
AU  - Yang M
FAU - Zhang, Rui
AU  - Zhang R
FAU - Zhuang, Caifang
AU  - Zhuang C
FAU - Wu, Yueyue
AU  - Wu Y
FAU - Yang, Qian
AU  - Yang Q
FAU - Yu, Zhiyuan
AU  - Yu Z
FAU - Liu, Jun
AU  - Liu J
FAU - Zha, Bingbing
AU  - Zha B
FAU - Gong, Qihai
AU  - Gong Q
FAU - Yang, Bo
AU  - Yang B
FAU - Zeng, Miao
AU  - Zeng M
FAU - Yan, Cuili
AU  - Yan C
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 0 (Methylamines)
RN  - 0 (Peptides)
RN  - 0 (Scorpion Venoms)
RN  - 0 (urotoxin)
RN  - FLD0K1SJ1A (trimethyloxamine)
SB  - IM
MH  - *Diabetes Mellitus, Type 2/urine
MH  - *Diabetic Nephropathies/urine
MH  - Female
MH  - Humans
MH  - Male
MH  - Methylamines
MH  - Peptides
MH  - Scorpion Venoms
EDAT- 2022/05/11 06:00
MHDA- 2022/05/14 06:00
CRDT- 2022/05/10 09:17
PHST- 2022/05/10 09:17 [entrez]
PHST- 2022/05/11 06:00 [pubmed]
PHST- 2022/05/14 06:00 [medline]
AID - 10.7754/Clin.Lab.2021.210836 [doi]
PST - ppublish
SO  - Clin Lab. 2022 May 1;68(5). doi: 10.7754/Clin.Lab.2021.210836.