PMID- 3553884
OWN - NLM
STAT- MEDLINE
DCOM- 19870522
LR  - 20211216
IS  - 0300-8584 (Print)
IS  - 0300-8584 (Linking)
VI  - 176
IP  - 2
DP  - 1987
TI  - Assay for heat-labile Escherichia coli enterotoxin using sandwich 
      erythroimmunoassay.
PG  - 89-97
AB  - We investigated the possibility of using a sheep erythrocyte-antibody conjugate 
      as reagent in a sandwich erythroimmunoassay (SERIA) procedure to detect and 
      titrate Escherichia coli heat-labile enterotoxin (LT) with the naked eye. In this 
      assay, which is based on the immunological similarity between Vibrio cholerae 
      toxin (CT) and LT, rabbit anti-CT IgG was used as immunosorbent, and sheep 
      erythrocytes, sensitized with the rabbit anti-CT antibodies, were used as 
      indicator. The sensitivity of the test was demonstrated by a comparative study 
      using an enzyme-linked immunosorbent assay (ELISA). The results obtained by SERIA 
      with 130 samples correlated well with those of a Vero cell assay and a GM1-ELISA. 
      The test is easy, relatively cheap and as sensitive as other standard techniques; 
      it is particularly suited for field laboratories, especially in tropical 
      countries, and a large number of strains may be examined daily.
FAU - Germani, Y
AU  - Germani Y
FAU - Begaud, E
AU  - Begaud E
FAU - Guesdon, J L
AU  - Guesdon JL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Med Microbiol Immunol
JT  - Medical microbiology and immunology
JID - 0314524
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Immune Sera)
RN  - 9012-63-9 (Cholera Toxin)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/*analysis
MH  - Cholera Toxin/immunology
MH  - Enterotoxins/*analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Erythrocytes
MH  - *Escherichia coli
MH  - *Escherichia coli Proteins
MH  - Immune Sera
MH  - Immunoassay
MH  - Sheep/blood
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1007/BF00200680 [doi]
PST - ppublish
SO  - Med Microbiol Immunol. 1987;176(2):89-97. doi: 10.1007/BF00200680.