PMID- 35539599
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 8
IP  - 11
DP  - 2018 Feb 2
TI  - Isolation, differentiation and biodiversity of ureolytic bacteria of Qatari soil 
      and their potential in microbially induced calcite precipitation (MICP) for soil 
      stabilization.
PG  - 5854-5863
LID - 10.1039/c7ra12758h [doi]
AB  - Biomineralization plays a key role in modifying the geological properties of 
      soil, thereby stabilizing it against wind erosion, especially in areas 
      characterized by harsh weather and harsh soil (calcareous and arid); i.e. Arabic 
      Gulf region. Among soil microorganisms, ureolytic bacteria are capable of 
      modifying soil characteristics and thus, inducing biomineralization. This 
      research investigated the occurrence and diversity of ureolytic bacteria in 
      Qatari soils, specifically to study their acquired potential to adapt to harsh 
      conditions exhibiting ureolytic activity. Soil samples were collected from 
      various locations in Qatar and were used to isolate the indigenous ureolytic 
      bacteria. It was noticed that most of the ureolytic bacteria in Qatari soil 
      belong to the genus Bacillus mainly Bacillus cereus. Identification and 
      differentiation of 18 ureolytic isolates were performed using MALDI-TOF MS 
      techniques while ribotyping (16S rRNA) molecular technique was used mainly for 6 
      selected strains. This study not only shows the diversity of species of ureolytic 
      bacteria in Qatari soil but also shows the diversity in their protein profiles, 
      which confirms that bacteria have adapted well to the harsh environment. In 
      addition, the strains were evaluated based on a newly modified screening method 
      in this work; i.e. production of arbitrary urease activity (AUA). Thus, the 
      strains showing the highest AUA, exhibited the highest capability to produce 
      urease enzymes induced by urea. Analysis of calcium carbonate precipitation 
      utilizing SEM-EDX showed that the ureolytic bacteria also play a significant role 
      in the precipitation of minerals such as CaCO(3), in the presence of urea in 
      soil. Therefore, this research showed a high occurrence of indigenous Bacillus 
      bacteria in Qatari soil that can perform biomineralization and thus can be 
      helpful, if properly stimulated, in enhancing soil stabilization, and for other 
      local applications as well, since they are adapted to these soil and weather 
      conditions.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Bibi, Shazia
AU  - Bibi S
AD  - Department of Biological and Environmental Sciences, College of Arts and 
      Sciences, Qatar University PO Box 2713 Doha Qatar Nabil.Zouari@qu.edu.qa 
      +974-4403-4559.
FAU - Oualha, Meriam
AU  - Oualha M
AD  - Department of Biological and Environmental Sciences, College of Arts and 
      Sciences, Qatar University PO Box 2713 Doha Qatar Nabil.Zouari@qu.edu.qa 
      +974-4403-4559.
FAU - Ashfaq, Mohammad Yousaf
AU  - Ashfaq MY
AD  - Department of Biological and Environmental Sciences, College of Arts and 
      Sciences, Qatar University PO Box 2713 Doha Qatar Nabil.Zouari@qu.edu.qa 
      +974-4403-4559.
FAU - Suleiman, Muhannad T
AU  - Suleiman MT
AD  - Department of Civil and Environmental Engineering, Lehigh University USA.
FAU - Zouari, Nabil
AU  - Zouari N
AUID- ORCID: 0000-0003-4652-1656
AD  - Department of Biological and Environmental Sciences, College of Arts and 
      Sciences, Qatar University PO Box 2713 Doha Qatar Nabil.Zouari@qu.edu.qa 
      +974-4403-4559.
LA  - eng
PT  - Journal Article
DEP - 20180206
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9078176
COIS- The authors declare no conflict of interest. All authors decided to publish this 
      work. The funding sponsors had no role in the design of the study; in the 
      collection, analyses, or interpretation of data; in the writing of the 
      manuscript, and in the decision to publish the results.
EDAT- 2018/02/06 00:00
MHDA- 2018/02/06 00:01
PMCR- 2018/02/06
CRDT- 2022/05/11 03:49
PHST- 2017/11/24 00:00 [received]
PHST- 2018/01/25 00:00 [accepted]
PHST- 2022/05/11 03:49 [entrez]
PHST- 2018/02/06 00:00 [pubmed]
PHST- 2018/02/06 00:01 [medline]
PHST- 2018/02/06 00:00 [pmc-release]
AID - c7ra12758h [pii]
AID - 10.1039/c7ra12758h [doi]
PST - epublish
SO  - RSC Adv. 2018 Feb 6;8(11):5854-5863. doi: 10.1039/c7ra12758h. eCollection 2018 
      Feb 2.