PMID- 35541948
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220512
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 8
IP  - 46
DP  - 2018 Jul 19
TI  - Immunomagnetic bead-based biotin-streptavidin system for highly efficient 
      detection of aflatoxin B(1) in agricultural products.
PG  - 26029-26035
LID - 10.1039/c8ra04460k [doi]
AB  - The potential homogeneous assay employing immunomagnetic beads (IMB) has been 
      receiving attention as a screening tool in food-safety control; the method is 
      simple, efficient, and does not require long incubation times or complex 
      separation steps. In this study, a homogeneous immunoassay has been successfully 
      developed and applied in the determination of aflatoxin B(1) (AFB(1)) 
      contamination in agricultural products by coupling IMB and the 
      biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit 
      of detection (LOD, IC(10)), half-maximal inhibition concentration (IC(50)) and 
      detection range (IC(20)-IC(80)) of BSA-IMB are 0.00579, 0.573 and 0.0183-17.9 ng 
      mL(-1), respectively, for AFB(1). The detection of AFB(1) by BSA-IMB can be 
      achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of 
      BSA-IMB with its analogues are negligible (<3.82%); these results indicate high 
      selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with 
      relative standard deviations (RSDs) of 3.4 to 13.2% for AFB(1) in agricultural 
      product samples. Furthermore, the results of BSA-IMB for authentic samples show 
      reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. 
      The proposed BSA-IMB system is demonstrated to be a satisfactory tool for 
      homogeneous, efficient, sensitive, and alternative detection of AFB(1) in a wide 
      detection range for agricultural product samples.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Li, Ming
AU  - Li M
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
FAU - Zhang, Yuanyuan
AU  - Zhang Y
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
FAU - Zhao, Rujin
AU  - Zhao R
AD  - Department of Environmental Engineering, School of the Environment and Safety 
      Engineering, Jiangsu University Zhenjiang 212013 P. R. China.
FAU - Liu, Zhenjiang
AU  - Liu Z
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
FAU - Hong, Xia
AU  - Hong X
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
FAU - Cui, Yin
AU  - Cui Y
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
FAU - Xue, Yonglai
AU  - Xue Y
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
FAU - Du, Daolin
AU  - Du D
AUID- ORCID: 0000-0003-1764-2205
AD  - Institute of Environment and Ecology, Institute of Environmental Health and 
      Ecological Security, School of the Environment and Safety Engineering, Jiangsu 
      University Zhenjiang 212013 P. R. China ddl@ujs.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180719
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9083124
COIS- There are no conflicts to declare.
EDAT- 2018/07/19 00:00
MHDA- 2018/07/19 00:01
PMCR- 2018/07/19
CRDT- 2022/05/11 04:05
PHST- 2018/05/25 00:00 [received]
PHST- 2018/07/01 00:00 [accepted]
PHST- 2022/05/11 04:05 [entrez]
PHST- 2018/07/19 00:00 [pubmed]
PHST- 2018/07/19 00:01 [medline]
PHST- 2018/07/19 00:00 [pmc-release]
AID - c8ra04460k [pii]
AID - 10.1039/c8ra04460k [doi]
PST - epublish
SO  - RSC Adv. 2018 Jul 19;8(46):26029-26035. doi: 10.1039/c8ra04460k. eCollection 2018 
      Jul 19.