PMID- 35542374 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220512 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) VI - 8 IP - 37 DP - 2018 Jun 5 TI - Biomimetic enzyme-linked immunoassay based on a molecularly imprinted 96-well plate for the determination of triazophos residues in real samples. PG - 20549-20556 LID - 10.1039/c8ra03531h [doi] AB - In this study, a direct competitive biomimetic enzyme-linked immune-sorbent assay (BELISA) based on a molecularly imprinted nanomembrane as an artificial antibody was developed for the determination of triazophos in real samples. The imprinted film was directly synthesized on the well surface of a 96-well plate using a dummy molecular template under the conditions of thermal polymerization. The developed BELISA using a hapten of triazophos as an enzyme-labeled probe is much more sensitive, simple, quick, steady and inexpensive than the other instrumental and immuno assay methods. Under optimal conditions, the linear range of the method was 0.001-10 000 mug L(-1) with a good regression coefficient of 0.977. The sensitivity (IC(50)) and the limit of detection (LOD) of BELISA were 428 mug L(-1) and 0.001 mug L(-1), respectively. This method was performed to detect triazophos in cabbage and apple samples, and showed excellent recovery and relative standard deviations (RSDs) ranging from 70.5 to 119.8% and from 5.2 to 19.7%, respectively. The results correlated well with those obtained using high performance liquid chromatography-tandem mass spectrometry. CI - This journal is (c) The Royal Society of Chemistry. FAU - Hong, Sihui AU - Hong S AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - She, Yongxin AU - She Y AUID- ORCID: 0000-0003-4039-9186 AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Cao, Xiaolin AU - Cao X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Wang, Miao AU - Wang M AUID- ORCID: 0000-0002-9576-5211 AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Zhang, Chao AU - Zhang C AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Zheng, Lufei AU - Zheng L AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Wang, Shanshan AU - Wang S AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Ma, Xingbin AU - Ma X AD - Institute of Veterinary and Animal Husbandry, Tibet Academy of Agricultural and Animal Husbandry Sciences Lhasa 850006 P. R. China. FAU - Shao, Hua AU - Shao H AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Jin, Maojun AU - Jin M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Jin, Fen AU - Jin F AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. FAU - Wang, Jing AU - Wang J AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Key Laboratory of Agro-Products Quality and Safety of Chinese Ministry of Agriculture Beijing 100081 P. R. China 0891syx@163.com +86 010 82106567 +86 10 82106513. LA - eng PT - Journal Article DEP - 20180605 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC9080809 COIS- All of the authors have declared no conflicts of interest. EDAT- 2018/06/05 00:00 MHDA- 2018/06/05 00:01 PMCR- 2018/06/05 CRDT- 2022/05/11 04:08 PHST- 2018/04/24 00:00 [received] PHST- 2018/05/23 00:00 [accepted] PHST- 2022/05/11 04:08 [entrez] PHST- 2018/06/05 00:00 [pubmed] PHST- 2018/06/05 00:01 [medline] PHST- 2018/06/05 00:00 [pmc-release] AID - c8ra03531h [pii] AID - 10.1039/c8ra03531h [doi] PST - epublish SO - RSC Adv. 2018 Jun 5;8(37):20549-20556. doi: 10.1039/c8ra03531h. eCollection 2018 Jun 5.