PMID- 35543448
OWN - NLM
STAT- MEDLINE
DCOM- 20220714
LR  - 20220714
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Linking)
VI  - 45
IP  - 8
DP  - 2022 Aug
TI  - Piscirickettsia salmonis forms a biofilm on nylon surface using a CDC Biofilm 
      Reactor.
PG  - 1099-1107
LID - 10.1111/jfd.13632 [doi]
AB  - Research into Piscirickettsia salmonis biofilms on materials commonly used in 
      salmon farming is crucial for understanding its persistence and virulence. We 
      used the CDC Biofilm Reactor to investigate P. salmonis (LF-89 and EM-90) biofilm 
      formation on Nylon, Stainless steel (316L), Polycarbonate and High-Density 
      Polyethylene (HDPE) surfaces. After 144 h of biofilm visualization by scanning 
      confocal laser microscopy under batch growth conditions, Nylon coupons generated 
      the greatest biofilm formation and coverage compared to Stainless steel (316L), 
      Polycarbonate and HDPE. Additionally, P. salmonis biofilm formation on Nylon was 
      significantly greater (p </= .01) than Stainless steel (316L), Polycarbonate and 
      HDPE at 288 h. We used Nylon coupons to determine the kinetic parameters of the 
      planktonic and biofilm phases of P. salmonis. The two strains had similar 
      latencies in the planktonic phase; however, LF-89 maximum growth was 2.5 orders 
      of magnitude higher (Log cell ml(-1) ). Additionally, LF-89 had a specified 
      growth rate (micromax) of 0.0177 +/- 0.006 h(-1) and a generation time of 39.2 h. This 
      study contributes to a deeper understanding of the biofilm formation by 
      P. salmonis and elucidates the impact of the biofilm on aquaculture systems.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Vidal, Jose Miguel
AU  - Vidal JM
AD  - Laboratorio de Biopeliculas y Microbiologia Ambiental, Centro de Biotecnologia, 
      Universidad de Concepcion, Concepcion, Chile.
AD  - Departamento de Investigacion y Desarrollo, Ecombio limitada, Concepcion, Chile.
FAU - Ruiz, Pamela
AU  - Ruiz P
AUID- ORCID: 0000-0003-0093-4439
AD  - Laboratorio de Biopeliculas y Microbiologia Ambiental, Centro de Biotecnologia, 
      Universidad de Concepcion, Concepcion, Chile.
AD  - Departamento de Ciencias Biologicas, Facultad de Ciencias de la Vida, Universidad 
      Andres Bello, Talcahuano, Chile.
FAU - Carrasco, Carlos
AU  - Carrasco C
AD  - Laboratorio de Biopeliculas y Microbiologia Ambiental, Centro de Biotecnologia, 
      Universidad de Concepcion, Concepcion, Chile.
FAU - Barros, Javier
AU  - Barros J
AD  - Departamento de Investigacion y Desarrollo, Micbiotech spa, Concepcion, Chile.
FAU - Sepulveda, Daniela
AU  - Sepulveda D
AD  - Laboratorio de Biopeliculas y Microbiologia Ambiental, Centro de Biotecnologia, 
      Universidad de Concepcion, Concepcion, Chile.
FAU - Ruiz-Tagle, Nathaly
AU  - Ruiz-Tagle N
AD  - Laboratorio de Biopeliculas y Microbiologia Ambiental, Centro de Biotecnologia, 
      Universidad de Concepcion, Concepcion, Chile.
FAU - Romero, Alex
AU  - Romero A
AD  - Laboratorio de Inmunologia y Estres de Organismos Acuaticos, Instituto de 
      Patologia Animal, Facultad de Ciencias Veterinarias, Universidad Austral de 
      Chile, Valdivia, Chile.
AD  - Interdisciplinary Center for Aquaculture Research, INCAR, Concepcion, Chile.
FAU - Urrutia, Homero
AU  - Urrutia H
AD  - Laboratorio de Biopeliculas y Microbiologia Ambiental, Centro de Biotecnologia, 
      Universidad de Concepcion, Concepcion, Chile.
AD  - Departamento de Microbiologia, Facultad de Ciencias Biologicas, Universidad de 
      Concepcion, Concepcion, Chile.
FAU - Oliver, Cristian
AU  - Oliver C
AD  - Laboratorio de Inmunologia y Estres de Organismos Acuaticos, Instituto de 
      Patologia Animal, Facultad de Ciencias Veterinarias, Universidad Austral de 
      Chile, Valdivia, Chile.
LA  - eng
GR  - Agencia Nacional de Investigacion y Desarrollo (ANID)/
GR  - 11180994/The Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT)/
GR  - 15110027/Fondo de Financiamiento de Centros de Investigacion (FONDAP) 
      Interdisciplinary Center for Aquaculture Research (INCAR)/
GR  - Vicerrectoria de Investigacion, Desarrollo y Creacion Artistica of the 
      Universidad Austral de Chile (VIDCA-UACh)/
PT  - Journal Article
DEP - 20220511
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
RN  - 0 (Nylons)
RN  - 12597-68-1 (Stainless Steel)
RN  - 9002-88-4 (Polyethylene)
RN  - Piscirickettsia salmonis
SB  - IM
MH  - Animals
MH  - Biofilms
MH  - Centers for Disease Control and Prevention, U.S.
MH  - *Fish Diseases/microbiology
MH  - Nylons
MH  - *Piscirickettsia
MH  - *Piscirickettsiaceae Infections/microbiology
MH  - Polyethylene
MH  - Stainless Steel
MH  - United States
OTO - NOTNLM
OT  - Piscirickettsia salmonis
OT  - CDC biofilm reactor
OT  - SRS
OT  - aquaculture
OT  - biofilm
EDAT- 2022/05/12 06:00
MHDA- 2022/07/15 06:00
CRDT- 2022/05/11 08:23
PHST- 2022/04/18 00:00 [revised]
PHST- 2022/01/05 00:00 [received]
PHST- 2022/04/21 00:00 [accepted]
PHST- 2022/05/12 06:00 [pubmed]
PHST- 2022/07/15 06:00 [medline]
PHST- 2022/05/11 08:23 [entrez]
AID - 10.1111/jfd.13632 [doi]
PST - ppublish
SO  - J Fish Dis. 2022 Aug;45(8):1099-1107. doi: 10.1111/jfd.13632. Epub 2022 May 11.