PMID- 35545344 OWN - NLM STAT- MEDLINE DCOM- 20220516 LR - 20240321 IS - 1672-7347 (Print) IS - 1672-7347 (Linking) VI - 47 IP - 4 DP - 2022 Apr 28 TI - Electroacupuncture in the treatment of neurogenic urine retention through autophagy mediated by AMPK/mTOR pathway. PG - 488-496 LID - 1672-7347(2022)04-0488-09 [pii] LID - 10.11817/j.issn.1672-7347.2022.210260 [doi] AB - OBJECTIVES: Electroacupuncture can enhance autophagic flow, promote neuronal regeneration, axonal and myelin remodeling to achieve the protection of spinal cord injury, but its role in neurogenic urine retention is not completely clear. This study aims to investigate whether the mechanism of electroacupuncture in the treatment of neurogenic urine retention is through autophagy mediated by adenosine monophosphate activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. METHODS: A rat model of neurogenic urine retention after sacral spinal cord injury was established. The rats with successful model were randomly divided into a model group, an electroacupuncture group (electro-acupuncture for Ciliao, Zhongji, and Sanyinjiao by electronic stimulation, once a day, 20 min each time for 7 days), and an electroacupuncture+AMP-activated protein kinase (AMPK) inhibitor group (on the basis of the treatment of electroacupuncture group, 100 mug of AMPK inhibitor compound C was injected intramuscularly around the L(2-3) intervertebral space on the 1st and 4th day). The normal group did not receive any treatment. The maximum bladder volume, bladder basal pressure, leak point pressure, and bladder compliance were recorded by multi-channel physiological recorder; the morphology of bladder tissue was observed by HE staining; autophagy was observed under transmission electron microscope; the expressions of LC3II and Beclin1 protein were observed by immunofluorescence staining; the protein levels of AMPK, phosphorylated-AMPK (p-AMPK), mTOR, phosphorylated-mTOR (p-mTOR), microtubule associated protein 1 light chain 3 (LC3) II and Beclin1 in bladder tissue were detected by Western blotting. RESULTS: Compared with the normal group, the maximum bladder capacity, leak point pressure, bladder compliance, p-AMPK, LC3II, Beclin1 protein expressions in the bladder tissue of the model group increased, and the p-mTOR protein expressions were decreased (all P<0.05); compared with the model group, the maximum bladder capacity, bladder compliance, p-mTOR protein expression in the bladder tissue of the electroacupuncture group were decreased, and the p-AMPK, LC3II, and Beclin1 protein expressions were increased (all P<0.05); compared with the electroacupuncture group, the maximum bladder capacity, bladder compliance, p-mTOR protein expression in the bladder tissue of the electroacupuncture+AMPK inhibitor group were increased, the p-AMPK, LC3II, and Beclin1 protein expressions were decreased (all P<0.05). In the model group, the bladder became larger, with unclear and varying degrees of degeneration, severe tissue damage and autophagosome appeared; the bladder of the electroacupuncture group was smaller than that of the model group, and all levels were clearly visible with autophagy bodies; the layers were slightly disordered and damaged in the electroacupuncture + AMPK inhibitor group. CONCLUSIONS: Electroacupuncture can activate autophagy through AMPK/mTOR pathway, thereby reducing neurogenic urine retention caused by spinal cord injury. FAU - Li, Zhengfei AU - Li Z AD - Department of Massage, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193. lizfe11@163.com. AD - National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China. lizfe11@163.com. FAU - Zhang, Ren AU - Zhang R AD - Department of Massage, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193. AD - National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China. FAU - Zhao, Guorui AU - Zhao G AD - Department of Massage, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193. AD - National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China. FAU - Kuang, Yao AU - Kuang Y AD - Department of Massage, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193. 18522006896@163.com. AD - National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China. 18522006896@163.com. LA - eng LA - chi GR - 2018KJ028/the Scientific Research Project of Tianjin Education Commission, China/ PT - Journal Article TT - 电针通过增强AMPK/mTOR通路介导的自噬治疗神经源性尿潴留. PL - China TA - Zhong Nan Da Xue Xue Bao Yi Xue Ban JT - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JID - 101230586 RN - 0 (Beclin-1) RN - EC 2.7.1.1 (mTOR protein, rat) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) SB - IM MH - AMP-Activated Protein Kinases MH - Animals MH - Autophagy MH - Beclin-1 MH - *Electroacupuncture MH - Mammals MH - Rats MH - Rats, Sprague-Dawley MH - *Spinal Cord Injuries MH - TOR Serine-Threonine Kinases PMC - PMC10930162 OAB - OBJECTIVE: Electroacupuncture can enhance autophagic flow, promote neuronal regeneration, axonal and myelin remodeling to achieve the protection of spinal cord injury, but its role in neurogenic urine retention is not completely clear. This study aims to investigate whether the mechanism of electroacupuncture in the treatment of neurogenic urine retention is through autophagy mediated by adenosine monophosphate activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. METHODS: A rat model of neurogenic urine retention after sacral spinal cord injury was established. The rats with successful model were randomly divided into a model group, an electroacupuncture group (electro-acupuncture for Ciliao, Zhongji, and Sanyinjiao by electronic stimulation, once a day, 20 min each time for 7 days), and an electroacupuncture+AMP-activated protein kinase (AMPK) inhibitor group (on the basis of the treatment of electroacupuncture group, 100 mug of AMPK inhibitor compound C was injected intramuscularly around the L(2-3) intervertebral space on the 1st and 4th day). The normal group did not receive any treatment. The maximum bladder volume, bladder basal pressure, leak point pressure, and bladder compliance were recorded by multi-channel physiological recorder; the morphology of bladder tissue was observed by HE staining; autophagy was observed under transmission electron microscope; the expressions of LC3II and Beclin1 protein were observed by immunofluorescence staining; the protein levels of AMPK, phosphorylated-AMPK (p-AMPK), mTOR, phosphorylated-mTOR (p-mTOR), microtubule associated protein 1 light chain 3 (LC3) II and Beclin1 in bladder tissue were detected by Western blotting. RESULTS: Compared with the normal group, the maximum bladder capacity, leak point pressure, bladder compliance, p-AMPK, LC3II, Beclin1 protein expressions in the bladder tissue of the model group increased, and the p-mTOR protein expressions were decreased (all P<0.05); compared with the model group, the maximum bladder capacity, bladder compliance, p-mTOR protein expression in the bladder tissue of the electroacupuncture group were decreased, and the p-AMPK, LC3II, and Beclin1 protein expressions were increased (all P<0.05); compared with the electroacupuncture group, the maximum bladder capacity, bladder compliance, p-mTOR protein expression in the bladder tissue of the electroacupuncture+AMPK inhibitor group were increased, the p-AMPK, LC3II, and Beclin1 protein expressions were decreased (all P<0.05). In the model group, the bladder became larger, with unclear and varying degrees of degeneration, severe tissue damage and autophagosome appeared; the bladder of the electroacupuncture group was smaller than that of the model group, and all levels were clearly visible with autophagy bodies; the layers were slightly disordered and damaged in the electroacupuncture + AMPK inhibitor group. CONCLUSION: Electroacupuncture can activate autophagy through AMPK/mTOR pathway, thereby reducing neurogenic urine retention caused by spinal cord injury. OABL- eng OTO - NOTNLM OT - adenine monophosphate activated protein kinase OT - autophagy OT - electroacupuncture OT - mammalian target of rapamycin OT - neurogenic urine retention COIS- 作者声称无任何利益冲突。 EDAT- 2022/05/12 06:00 MHDA- 2022/05/17 06:00 PMCR- 2022/04/28 CRDT- 2022/05/11 21:12 PHST- 2022/05/11 21:12 [entrez] PHST- 2022/05/12 06:00 [pubmed] PHST- 2022/05/17 06:00 [medline] PHST- 2022/04/28 00:00 [pmc-release] AID - 1672-7347(2022)04-0488-09 [pii] AID - 10.11817/j.issn.1672-7347.2022.210260 [doi] PST - ppublish SO - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2022 Apr 28;47(4):488-496. doi: 10.11817/j.issn.1672-7347.2022.210260.