PMID- 35545731
OWN - NLM
STAT- MEDLINE
DCOM- 20220627
LR  - 20220628
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 35
IP  - 4
DP  - 2022 Jul
TI  - Serum-derived extracellular vesicles mediate Smad4 expression through shuttling 
      microRNA-27a in the progression of laryngeal squamous cell carcinoma.
PG  - 1084-1099
LID - 10.1007/s13577-022-00712-6 [doi]
AB  - Serum-derived extracellular vesicles (EVs) containing non-coding RNAs have been 
      indicated to serve as diagnostic and prognostic biomarkers for laryngeal squamous 
      cell carcinoma (LSCC), while their functional role remains to be explored. Here, 
      we summarize the possible mechanism explaining the laryngeal carcinogenesis and 
      the associated changes with the involvement of extracellular microRNA (miR)-27a 
      from serum of LSCC patients. Serum-derived EVs from LSCC patients were found to 
      increase the proliferative activity and decreased the apoptotic activity of LSCC 
      cells. miRNA microarrays revealed that miR-27a expression was elevated after EV 
      treatment. miR-27a expression was elevated in LSCC tissues and predicted a poor 
      prognosis for patients. Downregulation of miR-27a inhibited the effect of EVs to 
      reduce the activity of LSCC cells in vitro and to suppress tumor development in 
      vivo. miR-27a targeted SMAD family member 4 (Smad4) to mediate the Wnt/beta-catenin 
      pathway, which was induced under the influence of EVs. Smad4 was downregulated in 
      LSCC tissues, and simultaneous overexpression of miR-27a and Smad4 resulted in 
      reduced cell activity and tumorigenicity. In conclusion, serum-derived EVs 
      support the laryngeal carcinogenesis at least partially via transferring miR-27a. 
      miR-27a targets Smad4 and is a biomarker to predict LSCC prognosis.
CI  - (c) 2022. The Author(s) under exclusive licence to Japan Human Cell Society.
FAU - Shuang, Yu
AU  - Shuang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, No. 23, Pingjiang Road, Tianjin, 300211, People's 
      Republic of China. yushuang_11252@126.com.
FAU - Yao, Xiaofeng
AU  - Yao X
AD  - Department of Maxillofacial and Otorhinolaryngology Head and Neck Surgery, 
      Tianjin Medical University Cancer Institute and Hospital, Tianjing, 300202, 
      People's Republic of China.
FAU - Liu, Jing
AU  - Liu J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, No. 23, Pingjiang Road, Tianjin, 300211, People's 
      Republic of China.
FAU - Niu, Juntao
AU  - Niu J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, No. 23, Pingjiang Road, Tianjin, 300211, People's 
      Republic of China.
FAU - Guo, Wenyu
AU  - Guo W
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, No. 23, Pingjiang Road, Tianjin, 300211, People's 
      Republic of China.
FAU - Li, Chao
AU  - Li C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, No. 23, Pingjiang Road, Tianjin, 300211, People's 
      Republic of China.
LA  - eng
GR  - 2020LC08/Clinical medicine research project of the second hospital of Tianjin 
      Medical University/
PT  - Journal Article
DEP - 20220512
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (MicroRNAs)
RN  - 0 (SMAD4 protein, human)
RN  - 0 (Smad4 Protein)
SB  - IM
MH  - Carcinogenesis/genetics
MH  - *Carcinoma, Squamous Cell/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Extracellular Vesicles/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics/metabolism/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - Smad4 Protein/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
OTO - NOTNLM
OT  - Extracellular vesicles
OT  - Laryngeal squamous cell carcinoma
OT  - Smad4
OT  - Wnt/beta-catenin pathway
OT  - microRNA-27a
EDAT- 2022/05/12 06:00
MHDA- 2022/06/28 06:00
CRDT- 2022/05/11 23:20
PHST- 2021/11/02 00:00 [received]
PHST- 2022/04/25 00:00 [accepted]
PHST- 2022/05/12 06:00 [pubmed]
PHST- 2022/06/28 06:00 [medline]
PHST- 2022/05/11 23:20 [entrez]
AID - 10.1007/s13577-022-00712-6 [pii]
AID - 10.1007/s13577-022-00712-6 [doi]
PST - ppublish
SO  - Hum Cell. 2022 Jul;35(4):1084-1099. doi: 10.1007/s13577-022-00712-6. Epub 2022 
      May 12.