PMID- 35546047
OWN - NLM
STAT- MEDLINE
DCOM- 20220517
LR  - 20220716
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 5
DP  - 2022 May
TI  - Cynarin suppresses gouty arthritis induced by monosodium urate crystals.
PG  - 11782-11793
LID - 10.1080/21655979.2022.2072055 [doi]
AB  - The study is aimed to determine the effects of cynarin (Cyn) on mice with gouty 
      arthritis (GA) induced by monosodium urate (MSU). We measured swelling in the 
      hind paws of mice in vivo using Vernier calipers and ultrasound. The liver, 
      kidney, and hind paws were stained with hematoxylin-eosin, and M1 type 
      macrophages were detected in the hind paws using anti-F4/80 and anti-iNOS 
      antibodies. The mRNA expression of inflammatory factors in bone marrow-derived 
      macrophages (BMDMs) and in the hind paws was detected via quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR). Nucleotide-binding 
      oligomerization domain (NOD)-like receptor (NLR) family pyrin domain containing 3 
      (NLRP3) inflammasomes and the nuclear factor kappa B (NF-kappaB) and 
      mitogen-activated protein kinase (MAPK) pathways were analyzed via western 
      blotting. Cyn was detected in vitro using Cell Counting Kit-8 (CCK-8). Cyn 
      treatment reduced hind paw swelling and M1 macrophage infiltration, suppressed 
      the mRNA expression of inflammatory factors, and inhibited NLRP3 inflammasome 
      activation in vivo, in addition to inhibiting the phosphorylation of IKKa/beta, p65, 
      and c-Jun NH 2-terminal kinase (JNK). Furthermore, Cyn exerted anti-inflammatory 
      and anti-swelling effects in mice with GA by regulating the NF-kappaB and JNK 
      pathways and NLRP3 inflammasomes.
FAU - Wu, Changgui
AU  - Wu C
AD  - Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
FAU - Chen, Shaohua
AU  - Chen S
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
AD  - Tianshan Hospital of Traditional Chinese Medicine, Shanghai, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
FAU - Kong, Bo
AU  - Kong B
AD  - Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Yan, Wei
AU  - Yan W
AD  - Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Tian, Hao
AU  - Tian H
AD  - Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Liu, Zhaoyi
AU  - Liu Z
AD  - Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western 
      Medicine, Shanghai, China.
FAU - Shi, Qi
AU  - Shi Q
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
AD  - Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education 
      (Shanghai University of Traditional Chinese Medicine), Shanghai, China.
FAU - Wang, Yongjun
AU  - Wang Y
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
AD  - Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education 
      (Shanghai University of Traditional Chinese Medicine), Shanghai, China.
FAU - Liang, Qianqian
AU  - Liang Q
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
AD  - Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education 
      (Shanghai University of Traditional Chinese Medicine), Shanghai, China.
FAU - Xi, Xiaobing
AU  - Xi X
AD  - Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Xu, Hao
AU  - Xu H
AD  - Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 
      China.
AD  - Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education 
      (Shanghai University of Traditional Chinese Medicine), Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (Cinnamates)
RN  - 0 (Inflammasomes)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (RNA, Messenger)
RN  - 268B43MJ25 (Uric Acid)
RN  - 85D81U9JAV (cynarine)
SB  - IM
MH  - Animals
MH  - *Arthritis, Gouty/chemically induced/drug therapy/metabolism
MH  - Cinnamates
MH  - Inflammasomes/metabolism
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/genetics/metabolism
MH  - RNA, Messenger
MH  - Uric Acid/adverse effects
PMC - PMC9275982
OTO - NOTNLM
OT  - Cynarin
OT  - JNK
OT  - NF-kappaB
OT  - NLRP3 inflammasomes
OT  - gouty arthritis
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/05/13 06:00
MHDA- 2022/05/18 06:00
PMCR- 2022/05/12
CRDT- 2022/05/12 06:33
PHST- 2022/05/12 06:33 [entrez]
PHST- 2022/05/13 06:00 [pubmed]
PHST- 2022/05/18 06:00 [medline]
PHST- 2022/05/12 00:00 [pmc-release]
AID - 2072055 [pii]
AID - 10.1080/21655979.2022.2072055 [doi]
PST - ppublish
SO  - Bioengineered. 2022 May;13(5):11782-11793. doi: 10.1080/21655979.2022.2072055.