PMID- 35547044
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230916
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 8
IP  - 61
DP  - 2018 Oct 10
TI  - miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell 
      apoptosis in vitro by targeting FOXO1.
PG  - 35031-35041
LID - 10.1039/c8ra06866f [doi]
AB  - OBJECTIVE: To investigate the role of FOXO1 and miR-183-96-182 clusters in ox-LDL 
      induced endothelial cell apoptosis. METHODS: FOXO1 overexpression (OE) and 
      knockdown (KD) as well as AKT1 OE in human umbilical vein endothelial cells 
      (HUVECs) and human aortic endothelial cells (HAECs) were achieved by lentiviral 
      transduction. Upregulation of miR-183-5p, miR-182-5p or miR-96-5p was mimicked by 
      agomir treatment. FOXO1 gene transcription was monitored by FOXO1 promotor 
      reporter assay. Cell apoptosis in culture was monitored by TiterTACS in situ 
      detection. Regulation of FOXO1 gene expression by an miRNA targeting mechanism 
      was monitored by AGO2-RNA immunoprecipitation assay. RESULTS: FOXO1 mRNA and 
      protein expression levels in ox-LDL treated HUVECs or HAECs were significantly 
      upregulated due to transcriptional and miRNA targeting mechanisms. MiR-183-5p, 
      miR-182-5p and miR-96-5p expression levels in HUVECs or HAECs were significantly 
      reduced by ox-LDL treatment, the overexpression of which by agomir treatment 
      partially reduced the FOXO1 mRNA/protein expression levels and cell apoptosis 
      which was upregulated by ox-LDL treatment. FOXO1 overexpression antagonized the 
      effect of the agomir treatment indicated above. MiR-183-5p, miR-182-5p and 
      miR-96-5p agomir treatment partially rescued the FOXO1 pSer256/total FOXO1 
      protein ratio and the AKT1 pSer473 level that were reduced by ox-LDL treatment in 
      the HUVECs or HAECs. AKT1 overexpression significantly reduced FOXO1 protein 
      expression, increased miR-182-5p and miR-183-5p expression, and partially 
      alleviated ox-LDL induced HUVEC or HAEC apoptosis in an miR-183-5p and 
      miR-182-5p-dependent manner. CONCLUSION: miR-183-96-182 clusters could partially 
      alleviate ox-LDL-induced apoptosis in HUVECs or HAECs by targeting FOXO1.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Liu, Zhi-Qin
AU  - Liu ZQ
AD  - Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Du, Jing-Jing
AU  - Du JJ
AD  - Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Ren, Jing-Jing
AU  - Ren JJ
AD  - Department of Hematology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Zhang, Zhi-Yong
AU  - Zhang ZY
AD  - Department of Neurology, China-Japan Friendship Hospital Beijing 100029 China.
FAU - Guo, Xiao-Bo
AU  - Guo XB
AD  - Department of Hematology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Yan, Yu-E
AU  - Yan YE
AD  - Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Jia, Xiao-Tao
AU  - Jia XT
AD  - Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Gu, Nai-Bing
AU  - Gu NB
AD  - Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Di, Zheng-Li
AU  - Di ZL
AD  - Department of Neurology, Xi'an Central Hospital, Xi'an Jiaotong University, 
      School of Medicine Xi'an 710003 Shaanxi China.
FAU - Li, San-Zhong
AU  - Li SZ
AUID- ORCID: 0000-0002-7570-6310
AD  - Department of Neurosurgery, Xi-jing Hospital Fourth Military Medical University 
      No. 127, Changle Xi Road Xi'an 710032 Shaanxi China lzqsjnk@126.com 
      +86-185-9140-9510.
LA  - eng
PT  - Journal Article
DEP - 20181012
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9087689
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2018/10/12 00:00
MHDA- 2018/10/12 00:01
PMCR- 2018/10/12
CRDT- 2022/05/13 11:08
PHST- 2018/08/16 00:00 [received]
PHST- 2018/09/17 00:00 [accepted]
PHST- 2022/05/13 11:08 [entrez]
PHST- 2018/10/12 00:00 [pubmed]
PHST- 2018/10/12 00:01 [medline]
PHST- 2018/10/12 00:00 [pmc-release]
AID - c8ra06866f [pii]
AID - 10.1039/c8ra06866f [doi]
PST - epublish
SO  - RSC Adv. 2018 Oct 12;8(61):35031-35041. doi: 10.1039/c8ra06866f. eCollection 2018 
      Oct 10.