PMID- 35548575
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220516
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 9
DP  - 2022
TI  - Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 
      Fraction From Panax Ginseng Berry Based on UHPLC-MS Metabolomics.
PG  - 865077
LID - 10.3389/fnut.2022.865077 [doi]
LID - 865077
AB  - OBJECTIVE: Ginseng berry (GB) was the mature fruit of medicinal and edible herb, 
      Panax ginseng C.A. Meyer, with significant hypoglycemic effect. Ginsenoside was 
      the main hypoglycemic active component of GB. Evaluating and screening the 
      effective components of GB was of great significance to further develop its 
      hypoglycemic effect. METHODS: The polar fractions of ginseng berry extract (GBE) 
      were separated by a solvent extraction, and identified by ultra-high performance 
      liquid chromatography-high-resolution mass spectrometry (UHPLC-MS). The insulin 
      resistance model of HepG2 cells was established, and the hypoglycemic active 
      fraction in GBE polar fractions were screened in vitro. Rat model of type 2 
      diabetes mellitus (T2DM) was established to verify the hypoglycemic effect of the 
      GBE active fraction. The metabolomic study based on UHPLC-MS was used to analyze 
      the differential metabolites in the serum of T2DM rats after 30 days of 
      intervention with hypoglycemic active GBE fraction. The kyoto encyclopedia of 
      genes and genomes (KEGG) metabolic pathway enrichment analysis was used to study 
      the main metabolic pathways involved in the regulation of hypoglycemic active 
      parts of GBE. RESULTS: It was found that GBE-5 fraction had better hypoglycemic 
      activity than other GBE polar fractions in vitro cell hypoglycemic activity 
      screening experiment. After 30 days of treatment, the fasting blood glucose value 
      of T2DM rats decreased significantly by 34.75%, indicating that it had 
      significant hypoglycemic effect. Eighteen differential metabolites enriched in 
      KEGG metabolic pathway were screened and identified in the rat serum from T2DM 
      vs. GBE-5 group, and the metabolic pathways mainly involved in regulation include 
      arachidonic acid (AA) metabolism, linoleic acid (LA) metabolism, unsaturated 
      fatty acid biosynthesis, and ferroptosis. CONCLUSIONS: The hypoglycemic effect of 
      GBE-5 fraction was better than that of total ginsenoside of GB. The AA 
      metabolism, LA metabolism, unsaturated fatty acid biosynthesis, and ferroptosis 
      were the potential metabolic pathways for GBE-5 fraction to exert hypoglycemic 
      regulation.
CI  - Copyright (c) 2022 Wang, Tong, Wang, Li, Lu, Li, Jiao and Wu.
FAU - Wang, Heyu
AU  - Wang H
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
AD  - School of Pharmacy, Jilin Medical University, Jilin, China.
FAU - Tong, Yu
AU  - Tong Y
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
FAU - Wang, Anqi
AU  - Wang A
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
FAU - Li, Ying
AU  - Li Y
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
FAU - Lu, Bofan
AU  - Lu B
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
FAU - Li, Hui
AU  - Li H
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
FAU - Jiao, Lili
AU  - Jiao L
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
FAU - Wu, Wei
AU  - Wu W
AD  - Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220425
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC9084362
OTO - NOTNLM
OT  - UHPLC-MS
OT  - ginseng berry
OT  - ginsenoside
OT  - metabolomics
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/05/14 06:00
MHDA- 2022/05/14 06:01
PMCR- 2022/01/01
CRDT- 2022/05/13 11:27
PHST- 2022/01/29 00:00 [received]
PHST- 2022/03/16 00:00 [accepted]
PHST- 2022/05/13 11:27 [entrez]
PHST- 2022/05/14 06:00 [pubmed]
PHST- 2022/05/14 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2022.865077 [doi]
PST - epublish
SO  - Front Nutr. 2022 Apr 25;9:865077. doi: 10.3389/fnut.2022.865077. eCollection 
      2022.