PMID- 35549817
OWN - NLM
STAT- MEDLINE
DCOM- 20220616
LR  - 20220616
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 142
IP  - 5
DP  - 2022 May
TI  - Fluorescence in situ hybridization and microbial community profiling analysis of 
      explanted cochlear implants.
PG  - 395-401
LID - 10.1080/00016489.2022.2070931 [doi]
AB  - BACKGROUND: Cochlear implant (CI) infections affect a small, but significant 
      number of patients. Unremitting infections can lead to explantation. Fluorescence 
      in situ hybridization (FISH) and microbial community profiling (MCP) are methods 
      of studying microbial environments of explanted devices that can provide 
      information to reduce morbidity and costs of infected CIs. AIMS/OBJECTIVES: To 
      describe the results and clinical significance of bacterial analyses conducted on 
      explanted CIs. MATERIAL AND METHODS: Between 2013 and 2017, 12 explanted devices 
      underwent microbiological analysis in addition to the manufacturer's device 
      failure analysis. Patients' clinical history, infection status and outcome were 
      reviewed and correlated with microbial analysis results. RESULTS: From 2013 to 
      2017, 12 Cochlear devices from 11 patients underwent additional MCP or FISH 
      analysis. Five devices were explanted due to suspected implant associated 
      infection, and seven were explanted for other reasons. FISH analysis revealed 
      biofilm presence on all infected devices, only partial correlation of cultures 
      with biofilm composition and confirmation that biofilm formation occurs 
      preferentially at particular device interfaces and geometries. MCP analysis 
      presented challenges in data analysis inherent to its technique but correlated 
      with cultures of infected devices and suggested a diverse microbial composition 
      of explanted devices. CONCLUSIONS AND SIGNIFICANCE: Microbial analysis of 
      explanted devices can aid in further elucidating treatment approaches to infected 
      CIs.
FAU - Asfour, Leena
AU  - Asfour L
AUID- ORCID: 0000-0003-0926-3803
AD  - Department of Otolaryngology-Head & Neck Surgery, New York University School of 
      Medicine, New York City, NY, USA.
FAU - Smyth, Daniel
AU  - Smyth D
AD  - Cochlear Limited, 1 University Avenue, Macquarie University, Sydney, Australia.
FAU - Whitchurch, Cynthia B
AU  - Whitchurch CB
AD  - The ithree Institute, University of Technology Sydney, Ultimo, Australia.
FAU - Cavaliere, Rosalia
AU  - Cavaliere R
AD  - The ithree Institute, University of Technology Sydney, Ultimo, Australia.
FAU - Roland, J Thomas Jr
AU  - Roland JT Jr
AD  - Department of Otolaryngology-Head & Neck Surgery, New York University School of 
      Medicine, New York City, NY, USA.
LA  - eng
PT  - Journal Article
DEP - 20220512
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
SB  - IM
MH  - Biofilms
MH  - *Cochlear Implantation/methods
MH  - *Cochlear Implants
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Microbiota
MH  - Postoperative Complications
OTO - NOTNLM
OT  - Cochlear implant infection
OT  - biofilm
OT  - fluorescence in situ hybridization
OT  - microbial analysis
OT  - microbial community profiling
EDAT- 2022/05/14 06:00
MHDA- 2022/06/18 06:00
CRDT- 2022/05/13 12:08
PHST- 2022/05/14 06:00 [pubmed]
PHST- 2022/06/18 06:00 [medline]
PHST- 2022/05/13 12:08 [entrez]
AID - 10.1080/00016489.2022.2070931 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2022 May;142(5):395-401. doi: 10.1080/00016489.2022.2070931. 
      Epub 2022 May 12.