PMID- 35550390
OWN - NLM
STAT- MEDLINE
DCOM- 20220525
LR  - 20220816
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 316
DP  - 2022 Jul 15
TI  - A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate 
      virulence and a promising application perspective.
PG  - 198801
LID - S0168-1702(22)00128-9 [pii]
LID - 10.1016/j.virusres.2022.198801 [doi]
AB  - NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV), which is 
      highly homologous to the NADC30 strain isolated in the United States. The 
      NADC30-like PRRSV was first reported in 2014 in China, where it spread and 
      gradually caused an epidemic. Currently, growing research has shown that 
      NADC30-like strains have greater propensity to recombine with other PRRSV 
      strains, particularly the PPRSV vaccine virus used clinically, making the 
      prevention and control of PRRSV highly complex. To carry out an in-depth 
      molecular biology and virulence analysis, a full-length infectious clone of the 
      NADC30-like strain was successfully constructed and rescued by reverse genetic 
      manipulation. The rescued virus, rZJqz, was indistinguishable from its parental 
      virus, ZJqz21, based on virological characteristics. Further animal experiments 
      demonstrated that rZJqz retained similar pathogenicity and induced the typical 
      clinical symptoms and viral shedding observed in the ZJqz21 challenge model. 
      Together, these results provide a useful tool for further study of the biological 
      characteristics and pathogenicity of NADC30-like strains. Moreover, these 
      findings also provide a solid foundation for studying the recombination of 
      different PRRSVs and developing new and effective universal vaccines in the 
      future.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Cao, Zhengda
AU  - Cao Z
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China; Shandong Agricultural University, Shandong, 271018, 
      China.
FAU - Chen, Jinxia
AU  - Chen J
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China.
FAU - Li, Liwei
AU  - Li L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China.
FAU - Liu, Jiachen
AU  - Liu J
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China.
FAU - Tong, Wu
AU  - Tong W
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, 
      Yangzhou, 225009, China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, 
      Yangzhou, 225009, China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, 
      Yangzhou, 225009, China.
FAU - Wang, Guihua
AU  - Wang G
AD  - Shandong Agricultural University, Shandong, 271018, China. Electronic address: 
      wguihua1126@163.com.
FAU - Gao, Fei
AU  - Gao F
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai, 200241, China; Jiangsu Co-Innovation Center for the Prevention and 
      Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, 
      Yangzhou, 225009, China. Electronic address: feigao@shvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220510
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - China/epidemiology
MH  - DNA Viruses
MH  - Genome, Viral
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Reverse Genetics
MH  - Swine
MH  - Virulence
MH  - *Viruses, Unclassified
OTO - NOTNLM
OT  - Full-length infectious clone
OT  - NADC30-like strain
OT  - Pathogenesis
OT  - Reverse genetic system
EDAT- 2022/05/14 06:00
MHDA- 2022/05/26 06:00
CRDT- 2022/05/13 12:37
PHST- 2022/02/22 00:00 [received]
PHST- 2022/05/06 00:00 [revised]
PHST- 2022/05/07 00:00 [accepted]
PHST- 2022/05/14 06:00 [pubmed]
PHST- 2022/05/26 06:00 [medline]
PHST- 2022/05/13 12:37 [entrez]
AID - S0168-1702(22)00128-9 [pii]
AID - 10.1016/j.virusres.2022.198801 [doi]
PST - ppublish
SO  - Virus Res. 2022 Jul 15;316:198801. doi: 10.1016/j.virusres.2022.198801. Epub 2022 
      May 10.