PMID- 3555476
OWN - NLM
STAT- MEDLINE
DCOM- 19870610
LR  - 20061115
IS  - 0232-766X (Print)
IS  - 0232-766X (Linking)
VI  - 45
IP  - 11-12
DP  - 1986
TI  - Regulatory mechanisms of intracellular proteolysis in mammalian cells.
PG  - 1575-83
AB  - Low molecular weight phosphoryl compounds, such as carbamoyl phosphate, 
      2,3-diphosphoglycerate and phytic acid protect, to different extents, 
      mitochondrial and cytosolic proteins such as ornithine transcarbamoylase (OTC), 
      carbamoyl phosphate synthetase (CPS), glutamate dehydrogenase (GDH) and 
      glyceraldehyde 3-phosphate dehydrogenase (GAPDH), from proteolytic inactivation 
      (rat liver lysosomal extracts, pronase, elastase). Given the wide variety and 
      common occurrence of low molecular weight reagents such as typified here, it 
      seems that this kind of inhibition may be important in the regulation of protein 
      turnover. Regulation of intracellular proteolysis can also occur via the 
      proteolytic systems. Immunocytochemical procedures for mitochondrial enzymes 
      (CPS, GDH, OTC), show intracellular homogeneity, but intercellular heterogeneity 
      in rat liver, compatible with a role of the autophagic-lysosomal system in 
      degrading these proteins. However, degradation of short-lived proteins occurs by 
      other mechanisms. Using centrifugation of cultured cells, we find that the Golgi 
      apparatus takes part in the degradation of these proteins, probably by 
      controlling the traffic of proteins or proteases to the degradation site.
FAU - Knecht, E
AU  - Knecht E
FAU - Roche, E
AU  - Roche E
FAU - Hernandez-Yago, J
AU  - Hernandez-Yago J
FAU - Vargas, J L
AU  - Vargas JL
FAU - Grisolia, S
AU  - Grisolia S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biomed Biochim Acta
JT  - Biomedica biochimica acta
JID - 8304435
RN  - 0 (Proteins)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Animals
MH  - Biotransformation
MH  - Cells, Cultured
MH  - Golgi Apparatus/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Hydrolysis
MH  - Immunochemistry
MH  - Microscopy, Electron
MH  - Peptide Hydrolases/*metabolism
MH  - Proteins/metabolism
MH  - Rats
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
PST - ppublish
SO  - Biomed Biochim Acta. 1986;45(11-12):1575-83.