PMID- 35559382
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220516
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 14
IP  - 4
DP  - 2022
TI  - Circ_MTM1 knockdown inhibits the progression of HBV-related liver fibrosis via 
      regulating IL7R expression through targeting miR-122-5p.
PG  - 2199-2211
AB  - BACKGROUND AND OBJECTIVE: Hepatitis B virus (HBV) infection is the main reason 
      for liver cirrhosis. The purpose of this research was to probe into the role and 
      underlying mechanism of circ_myotubularin 1 (circ_MTM1) in HBV-related liver 
      fibrosis (LF). METHODS: HBV surface antigen (HBsAg) and e antigen (HBeAg), as 
      well as the levels of HBV DNA and HBV covalently closed circular DNA were 
      measured by HBsAg and HBeAg ELISA kits or RT-qPCR. Western blot or 
      immunohistochemistry assays were conducted to measure protein levels. The 
      expression of circ_MTM1, microRNA-122-5p (miR-122-5p) and interleukin 7 receptor 
      (IL7R) were measured using RT-qPCR. MTT and cell colony formation assays were 
      performed to detect cell proliferation. In vivo assays were carried out to reveal 
      the effect of circ_MTM1 silencing on the tumor growth in HBV-related 
      hepatocellular carcinoma (HCC). RESULTS: Circ_MTM1 and IL7R were highly 
      expressed, whereas miR-122-5p was lowly expressed in HBV-infected LX-2 cells. 
      Circ_MTM1 knockdown inhibited the progression of HBV-related LF. Circ_MTM1 could 
      target miR-122-5p to regulate the expression of IL7R by adsorbing miR-122-5p, 
      thus mediating the progression of HBV-related LF. Circ_MTM1 silencing repressed 
      cell proliferation of HepG2.2.15 cells and growth of HCC. CONCLUSION: Circ_MTM1 
      could serve as a promoter in HBV-related LF through miR-122-5p/IL7R axis.
CI  - AJTR Copyright (c) 2022.
FAU - Li, Bin
AU  - Li B
AD  - Department of Pathogenic Biology, School of Basic Medicine, Jinzhou Medical 
      University Jinzhou 121001, Liaoning, China.
FAU - Li, Yonggang
AU  - Li Y
AD  - Department of Pathogenic Biology, School of Basic Medicine, Jinzhou Medical 
      University Jinzhou 121001, Liaoning, China.
FAU - Li, Shuhua
AU  - Li S
AD  - Laboratory of Immunology and Pathogenic Biology, Experimental Teaching Center of 
      Basic Medicine, Jinzhou Medical University Jinzhou 121001, Liaoning, China.
FAU - Li, Hongwei
AU  - Li H
AD  - Laboratory of Immunology and Pathogenic Biology, Experimental Teaching Center of 
      Basic Medicine, Jinzhou Medical University Jinzhou 121001, Liaoning, China.
FAU - Liu, Ling
AU  - Liu L
AD  - Laboratory of Immunology and Pathogenic Biology, Experimental Teaching Center of 
      Basic Medicine, Jinzhou Medical University Jinzhou 121001, Liaoning, China.
FAU - Yu, Haiying
AU  - Yu H
AD  - Laboratory of Ergology, Experimental Teaching Center of Basic Medicine, Jinzhou 
      Medical University Jinzhou 121001, Liaoning, China.
LA  - eng
PT  - Journal Article
DEP - 20220415
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC9091097
OTO - NOTNLM
OT  - Hepatitis B virus
OT  - IL7R
OT  - circ_MTM1
OT  - miR-122-5p
COIS- None.
EDAT- 2022/05/14 06:00
MHDA- 2022/05/14 06:01
PMCR- 2022/04/15
CRDT- 2022/05/13 18:26
PHST- 2021/07/19 00:00 [received]
PHST- 2022/03/17 00:00 [accepted]
PHST- 2022/05/13 18:26 [entrez]
PHST- 2022/05/14 06:00 [pubmed]
PHST- 2022/05/14 06:01 [medline]
PHST- 2022/04/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2022 Apr 15;14(4):2199-2211. eCollection 2022.