PMID- 35560736
OWN - NLM
STAT- MEDLINE
DCOM- 20220614
LR  - 20220614
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 36
IP  - 14
DP  - 2022 Jul 30
TI  - Screening and identifying cucurbitacins and cucurbitacin glycosides in Cucumis 
      sativus using high-performance liquid chromatography/quadrupole-time-of-flight 
      mass spectrometry combined with in-source fragmentation and alkali adduct ions.
PG  - e9323
LID - 10.1002/rcm.9323 [doi]
AB  - RATIONALE: Cucumber, as a popular fruit and vegetable, has tremendously 
      contributed to providing a sufficient and high-quality food supply. However, the 
      cucumber plant metabolites, which may possess potential benefits for human 
      health, were rarely reported. In addition, rapid detection of these metabolites 
      from the complex biological matrix of cucumber samples is a tremendous challenge. 
      METHODS: A rapid detection method was established to systematically screen 
      cucurbitacins and cucurbitacin glycosides in cucumber plants by combining 
      high-performance liquid chromatography/quadrupole-time-of-flight mass 
      spectrometry (HPLC-Q-TOF-MS) with in-source fragmentation (ISF). Moreover, the 
      alkali cations, including acetic acid, 0.1% LiCl, 0.1% NH(4) Cl, 0.1% NaCl, and 
      0.1% KCl, were added to the mobile phase for improving the ion response. RESULTS: 
      The fragmentation pathways of seven cucurbitacins and cucurbitacin glycosides 
      were primarily investigated. The characteristic ISF ions at m/z 501.3211 and 
      503.3367 were identified and employed to screen 40 cucurbitacins and 
      cucurbitacin glycosides from the complex biological matrix. Their structures were 
      identified by their tandem mass spectrometry (MS/MS) spectra and fragmentation 
      pathways of references. Finally, the metabolic distribution and network of 
      cucurbitacins and cucurbitacin glycosides in cucumber plants were also proposed. 
      CONCLUSIONS: This work marks the first systematic and comprehensive study of the 
      metabolites in cucumber plants using HPLC-Q-TOF-MS technology, providing a 
      template for screening and identifying the triterpenoids from other plant-derived 
      medicines or food.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Chen, Si-Yuan
AU  - Chen SY
AUID- ORCID: 0000-0002-3790-0833
AD  - The First Hospital of Hunan University of Chinese Medicine, Hunan University of 
      Chinese Medicine, Changsha, China.
FAU - Zhou, Qing Yi-Jun
AU  - Zhou QY
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, 
      China.
FAU - Chen, Lin
AU  - Chen L
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, 
      China.
FAU - Li, Jia-Yu
AU  - Li JY
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, 
      China.
FAU - Xie, Tao
AU  - Xie T
AD  - Department of Acupuncture and Rehabilitation, Changsha Traditional Chinese 
      Medicine Hospital, Changsha, China.
FAU - Zhang, Shui-Han
AU  - Zhang SH
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, 
      China.
LA  - eng
GR  - 2021SF5045/The Special Funds for Development of Local Science and Technology from 
      Central Government/
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Alkalies)
RN  - 0 (Glycosides)
RN  - 0 (Ions)
RN  - 60137-06-6 (Cucurbitacins)
SB  - IM
MH  - Alkalies
MH  - Chromatography, High Pressure Liquid/methods
MH  - *Cucumis sativus
MH  - *Cucurbitacins/analysis
MH  - Glycosides/chemistry
MH  - Humans
MH  - Ions
MH  - Tandem Mass Spectrometry/methods
EDAT- 2022/05/14 06:00
MHDA- 2022/06/15 06:00
CRDT- 2022/05/13 19:18
PHST- 2022/04/15 00:00 [revised]
PHST- 2022/03/05 00:00 [received]
PHST- 2022/05/09 00:00 [accepted]
PHST- 2022/05/14 06:00 [pubmed]
PHST- 2022/06/15 06:00 [medline]
PHST- 2022/05/13 19:18 [entrez]
AID - 10.1002/rcm.9323 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2022 Jul 30;36(14):e9323. doi: 10.1002/rcm.9323.