PMID- 35565931
OWN - NLM
STAT- MEDLINE
DCOM- 20220519
LR  - 20220716
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 14
IP  - 9
DP  - 2022 May 7
TI  - Identification of Allergens in White- and Red-Fleshed Pitaya (Selenicereus 
      undatus and Selenicereus costaricensis) Seeds Using Bottom-Up Proteomics Coupled 
      with Immunoinformatics.
LID - 10.3390/nu14091962 [doi]
LID - 1962
AB  - White-fleshed pitaya (Selenicereus undatus) and red-fleshed pitaya (Selenicereus 
      costaricensis) are becoming increasingly popular because of their nutritional and 
      medicinal benefits. However, in addition to their beneficial properties, allergy 
      to pitaya fruits has occurred in daily life. In this study, we investigated the 
      protein profile of pitaya fruit seeds and focused on the most reactive proteins 
      against immunoglobulin E (IgE) in sera from allergic patients by immunoblotting. 
      A protein band of approximately 20 kDa displayed a clear reaction with the serum 
      IgE. The protein bands of interest were excised, in-gel digested, and analyzed 
      using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by data 
      searching against a restricted database (Caryophyllales in UniProtKB) for protein 
      identification. Immunoinformatic tools were used to predict protein 
      allergenicity. The potential allergens included cupin_1 and heat shock protein 70 
      (HSP70) in white-fleshed pitaya seeds, and cupin_1, heat shock protein 70, and 
      heat shock protein sti1-like in red-fleshed pitaya seeds are potential allergens. 
      The expression of potential allergens was further verified at the transcriptional 
      level in the species of S. undatus and S. costaricensis.
FAU - Hao, Mengzhen
AU  - Hao M
AD  - College of Food Science and Nutritional Engineering, China Agriculture 
      University, Beijing 100083, China.
FAU - Xijiri
AU  - Xijiri
AD  - College of Food Science and Nutritional Engineering, China Agriculture 
      University, Beijing 100083, China.
FAU - Zhao, Ziyi
AU  - Zhao Z
AD  - College of Food Science and Nutritional Engineering, China Agriculture 
      University, Beijing 100083, China.
FAU - Che, Huilian
AU  - Che H
AUID- ORCID: 0000-0002-1632-5729
AD  - College of Food Science and Nutritional Engineering, China Agriculture 
      University, Beijing 100083, China.
LA  - eng
GR  - 7202100/Natural Science Foundation of Beijing Municipality/
PT  - Journal Article
DEP - 20220507
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Allergens)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Allergens/analysis
MH  - *Cactaceae/chemistry
MH  - Chromatography, Liquid
MH  - HSP70 Heat-Shock Proteins
MH  - Humans
MH  - Immunoglobulin E
MH  - Proteomics
MH  - Seeds/chemistry
MH  - Tandem Mass Spectrometry
PMC - PMC9134757
OTO - NOTNLM
OT  - IgE-binding proteins
OT  - Selenicereus costaricensis
OT  - Selenicereus undatus
OT  - food allergen
OT  - pitaya seeds
OT  - proteomics
COIS- The funders had no role in the design of the study; in the collection, analysis, 
      or interpretation of data; in the writing of the manuscript; or in the decision 
      to publish the results.
EDAT- 2022/05/15 06:00
MHDA- 2022/05/20 06:00
PMCR- 2022/05/07
CRDT- 2022/05/14 01:22
PHST- 2022/04/02 00:00 [received]
PHST- 2022/04/29 00:00 [revised]
PHST- 2022/05/05 00:00 [accepted]
PHST- 2022/05/14 01:22 [entrez]
PHST- 2022/05/15 06:00 [pubmed]
PHST- 2022/05/20 06:00 [medline]
PHST- 2022/05/07 00:00 [pmc-release]
AID - nu14091962 [pii]
AID - nutrients-14-01962 [pii]
AID - 10.3390/nu14091962 [doi]
PST - epublish
SO  - Nutrients. 2022 May 7;14(9):1962. doi: 10.3390/nu14091962.