PMID- 35567596 OWN - NLM STAT- MEDLINE DCOM- 20231004 LR - 20231004 IS - 1573-6822 (Electronic) IS - 0742-2091 (Linking) VI - 39 IP - 5 DP - 2023 Oct TI - CircZNF609 promotes bladder cancer progression and inhibits cisplatin sensitivity via miR-1200/CDC25B pathway. PG - 1-18 LID - 10.1007/s10565-022-09715-3 [doi] AB - Circular RNAs (circRNAs) have been extensively studied in tumor development and treatment. CircZNF609 (hsa_circ_0000615) has been shown to serve as an oncogene in all kinds of solid tumors and may act as the novel biomarker in tumor diagnosis and therapy in tumor early diagnosis and therapy. However, the underlying character and mechanism of circZNF609 in cisplatin chemosensitivity and bladder cancer (BCa) development were unknown. The expression level of cell division cycle 25B (CDC25B), microRNA 1200 (miR-1200), and circZNF609 in BCa cells and tissues depended on quantitative real-time PCR (qRT-PCR). CDC25B protein level was assayed with Western blot. Functional assays in vitro and in vivo had been conducted to inspect the important role of circZNF609 on BCa progression and cisplatin chemosensitivity in BCa. RNA sequencing and online databases were used to predict the interactions among circZNF609, miR-1200, and CDC25B. Mechanistic exploration was confirmed by RNA pull-down assay, RNA fluorescence in situ hybridization (FISH) and Dual luciferase reporter assay. CircZNF609 expression was increased significantly in BCa cell lines and tissues. For BCa patients, increased expression of circZNF609 was correlated with a worse survival. In vitro and in vivo, enforced expression of circZNF609 enhanced BCa cells proliferation, migration, and cisplatin chemoresistance. Mechanistically, circZNF609 alleviated the inhibition effect on target CDC25B expression by sponging miR-1200. CircZNF609 promoted tumor growth through novel circZNF609/miR-1200/CDC25B axis, implying that circZNF609 has significant potential to act as a new diagnostic biomarker and therapeutic target in BCa. Enhancing cisplatin sensitivity is an important direction for bladder cancer management. 1. This research reveals that circZNF609 improves bladder cancer progression and inhibits cisplatin sensitivity by inducing G1/S cell cycle arrest via a novel miR-1200/CDC25B cascades. 2. CircZNF609 was confirmed associated with worse survival of bladder cancer patients. 3. CircZNF609 act as a prognostic biomarker for bladder cancer treatment. CI - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Feng, Dexiang AU - Feng D AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Lv, Jiancheng AU - Lv J AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Li, Kai AU - Li K AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Cao, Qiang AU - Cao Q AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Han, Jie AU - Han J AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Yu, Hao AU - Yu H AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Cheng, Yidong AU - Cheng Y AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Zhuang, Juntao AU - Zhuang J AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Cai, Lingkai AU - Cai L AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. FAU - Yang, Haiwei AU - Yang H AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. haiweiyang@njmu.edu.cn. FAU - Yang, Xiao AU - Yang X AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. yangxiao2915@163.com. FAU - Lu, Qiang AU - Lu Q AUID- ORCID: 0000-0003-3589-2507 AD - Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. doctorlvqiang@njmu.edu.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220514 PL - Switzerland TA - Cell Biol Toxicol JT - Cell biology and toxicology JID - 8506639 RN - Q20Q21Q62J (Cisplatin) RN - 0 (MicroRNAs) RN - 0 (RNA, Circular) RN - EC 3.1.3.48 (CDC25B protein, human) RN - EC 3.1.3.48 (cdc25 Phosphatases) SB - IM MH - Humans MH - Cisplatin/pharmacology/therapeutic use MH - *MicroRNAs/genetics/metabolism MH - In Situ Hybridization, Fluorescence MH - *Urinary Bladder Neoplasms/drug therapy/genetics/metabolism MH - RNA, Circular/genetics/metabolism MH - Cell Proliferation/genetics MH - Cell Line, Tumor MH - Gene Expression Regulation, Neoplastic MH - cdc25 Phosphatases/genetics/metabolism OTO - NOTNLM OT - Bladder cancer OT - Circular RNA OT - Cisplatin OT - Migration OT - Proliferation EDAT- 2022/05/15 06:00 MHDA- 2023/10/04 06:44 CRDT- 2022/05/14 11:02 PHST- 2021/11/20 00:00 [received] PHST- 2022/04/07 00:00 [accepted] PHST- 2023/10/04 06:44 [medline] PHST- 2022/05/15 06:00 [pubmed] PHST- 2022/05/14 11:02 [entrez] AID - 10.1007/s10565-022-09715-3 [pii] AID - 10.1007/s10565-022-09715-3 [doi] PST - ppublish SO - Cell Biol Toxicol. 2023 Oct;39(5):1-18. doi: 10.1007/s10565-022-09715-3. Epub 2022 May 14.