PMID- 35569658
OWN - NLM
STAT- MEDLINE
DCOM- 20220615
LR  - 20220615
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 838
IP  - Pt 1
DP  - 2022 Sep 10
TI  - Metabolomic disorders unveil hepatotoxicity of environmental microplastics in 
      wild fish Serranus scriba (Linnaeus 1758).
PG  - 155872
LID - S0048-9697(22)02969-2 [pii]
LID - 10.1016/j.scitotenv.2022.155872 [doi]
AB  - Coastal areas are worldwide subject to large inputs of anthropogenic wastes that 
      are discharged directly into inshore waters, where they will be weathered into 
      small microplastics (MPs) of up to a size <20 mum. This study provides information 
      about the presence of small environmental MPs (</=3 mum) in the liver of adult 
      benthopelagic fish Serranus scriba (Linnaeus 1758), caught from three coastal 
      regions in Tunisia distinguished by different patterns of human activity. Polymer 
      composition in fish liver was identified using Raman microspectroscopy. Results 
      revealed differences in the abundance, size distribution and presence of plastic 
      additives over the investigated sites. Polyethylene-vinyl acetate (PEVA: 34% 
      particles/g of tissue), high density polyethylene (HDPE: 24.4%) and the two 
      smaller size classes, i.e. 3-1.2 mum and 1.2-0.45 mum, were the most abundant MPs 
      types and size distribution found, respectively, in Bizerte channel (BC) site 
      (Bizerte city, Tunisia). Moreover, at hepatic level data showed a significant 
      site-dependent cytotoxicity expressed by changes in malondialdehyde (MDA) 
      content, presence of reactive oxygen species (ROS) expressed by altered level of 
      catalase (CAT) and glutathione-S-transferase (GST) activities and in the content 
      of metallothioneins (MTs), as well as genotoxicity by changes in the amount of 
      micronucleus (MN), and neurotoxicity by altered activity of acetylcholinesterase 
      (AChE). A innovative metabolomics analysis was also performed to further 
      investigate the distinct patterns of key metabolite changes in the liver of 
      Serranus scriba. A total of 36 metabolites were significantly affected, mainly 
      involved in energy, amino acid and osmolyte metabolism. These findings emphasised 
      for the first time a close relationship between the source, abundance and size 
      ranges of environmental MPs </= 3 mum and their hepatotoxicity in wild organisms.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Zitouni, Nesrine
AU  - Zitouni N
AD  - Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of 
      Agronomy, University of Sousse, Tunisia; University of Monastir, Higher Institute 
      of Biotechnology of Monastir, Monastir, Tunisia.
FAU - Cappello, Tiziana
AU  - Cappello T
AD  - University of Messina, Department of Chemical, Biological, Pharmaceutical and 
      Environmental Sciences, 98166 Messina, Italy. Electronic address: 
      tcappello@unime.it.
FAU - Missawi, Omayma
AU  - Missawi O
AD  - Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of 
      Agronomy, University of Sousse, Tunisia; University of Monastir, Higher Institute 
      of Biotechnology of Monastir, Monastir, Tunisia.
FAU - Boughattas, Iteb
AU  - Boughattas I
AD  - Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of 
      Agronomy, University of Sousse, Tunisia.
FAU - De Marco, Giuseppe
AU  - De Marco G
AD  - University of Messina, Department of Chemical, Biological, Pharmaceutical and 
      Environmental Sciences, 98166 Messina, Italy.
FAU - Belbekhouche, Sabrina
AU  - Belbekhouche S
AD  - Paris-Est Institute of Chemistry and Materials, UMR 7182 CNRS, Universite 
      Paris-Est Creteil Val-de-Marne, 2 rue Henri Dunant, 94320 Thiais, France.
FAU - Mokni, Moncef
AU  - Mokni M
AD  - Department of Pathology, CHU Farhat Hached, Sousse, Tunisia.
FAU - Alphonse, Vanessa
AU  - Alphonse V
AD  - Laboratory Water, Environment and Urban Systems, University Paris-Est Creteil, 
      Faculty of Science and Technology, Creteil Cedex, France.
FAU - Guerbej, Hamadi
AU  - Guerbej H
AD  - Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), National 
      Institute of Marine Sciences and Technologies (INSTM), Monastir Center, Monastir, 
      Tunisia.
FAU - Bousserrhine, Noureddine
AU  - Bousserrhine N
AD  - Laboratory Water, Environment and Urban Systems, University Paris-Est Creteil, 
      Faculty of Science and Technology, Creteil Cedex, France.
FAU - Banni, Mohamed
AU  - Banni M
AD  - Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of 
      Agronomy, University of Sousse, Tunisia; University of Monastir, Higher Institute 
      of Biotechnology of Monastir, Monastir, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20220513
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9002-88-4 (Polyethylene)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - *Bass/metabolism
MH  - *Chemical and Drug Induced Liver Injury
MH  - Environmental Monitoring
MH  - Metabolomics
MH  - Microplastics
MH  - Plastics/toxicity
MH  - Polyethylene/analysis
MH  - *Water Pollutants, Chemical/analysis/toxicity
OTO - NOTNLM
OT  - Benthopelagic fish
OT  - Coastal pollution
OT  - Ecotoxicological endpoints
OT  - Liver
OT  - Microplastic polymers
OT  - NMR-based metabolomics
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/05/16 06:00
MHDA- 2022/06/16 06:00
CRDT- 2022/05/15 19:25
PHST- 2022/03/15 00:00 [received]
PHST- 2022/05/07 00:00 [revised]
PHST- 2022/05/08 00:00 [accepted]
PHST- 2022/05/16 06:00 [pubmed]
PHST- 2022/06/16 06:00 [medline]
PHST- 2022/05/15 19:25 [entrez]
AID - S0048-9697(22)02969-2 [pii]
AID - 10.1016/j.scitotenv.2022.155872 [doi]
PST - ppublish
SO  - Sci Total Environ. 2022 Sep 10;838(Pt 1):155872. doi: 
      10.1016/j.scitotenv.2022.155872. Epub 2022 May 13.