PMID- 35570267
OWN - NLM
STAT- MEDLINE
DCOM- 20220517
LR  - 20220716
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 19
IP  - 1
DP  - 2022 May 15
TI  - Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA 
      enhances porcine reproductive and respiratory syndrome virus-2 replication.
PG  - 82
LID - 10.1186/s12985-022-01811-4 [doi]
LID - 82
AB  - BACKGROUND: Heterogeneous nuclear ribonucleoprotein (HnRNP) F is a member of 
      HnRNP family proteins that participate in splicing of cellular newly synthesized 
      mRNAs by specifically recognizing tandem guanine-tracts (G-tracts) RNA sequences. 
      Whether HnRNP F could recognize viral-derived tandem G-tracts and affect virus 
      replication remain poorly defined. METHODS: The effect of HnRNP F on porcine 
      reproductive and respiratory syndrome virus (PRRSV) propagation was evaluated by 
      real-time PCR, western blotting, and plaque-forming unit assay. The association 
      between HnRNP F and PRRSV guanine-rich segments (GRS) were analyzed by RNA 
      pulldown and RNA immunoprecipitation. The expression pattern of HnRNP F was 
      investigated by western blotting and nuclear and cytoplasmic fractionation. 
      RESULTS: Knockdown of endogenous HnRNP F effectively blocks the synthesis of 
      viral RNA and nucleocapsid (N) protein. Conversely, overexpression of porcine 
      HnRNP F has the opposite effect. Moreover, RNA pulldown and RNA 
      immunoprecipitation assays reveal that the qRMM1 and qRRM2 domains of HnRNP F 
      recognize the GRS in PRRSV antigenomic RNA. Finally, HnRNP F is redistributed 
      into the cytoplasm and forms a complex with guanine-quadruplex (G4) helicase 
      DHX36 during PRRSV infection. CONCLUSIONS: These findings elucidate the potential 
      functions of HnRNP F in regulating the proliferation of PRRSV and contribute to a 
      better molecular understanding of host-PRRSV interactions.
CI  - (c) 2022. The Author(s).
FAU - Zhang, Aiguo
AU  - Zhang A
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
FAU - Sun, Yanting
AU  - Sun Y
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
FAU - Jing, Huiyuan
AU  - Jing H
AUID- ORCID: 0000-0003-3158-9834
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. 
      lhsjhy@126.com.
FAU - Liu, Jie
AU  - Liu J
AD  - College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 
      610041, China.
FAU - Duan, Erzhen
AU  - Duan E
AD  - College of Biological Engineering, Henan University of Technology, Zhengzhou, 
      450001, China.
FAU - Ke, Wenting
AU  - Ke W
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Tao, Ran
AU  - Tao R
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Li, Yang
AU  - Li Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Wang, Jinhe
AU  - Wang J
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
FAU - Cao, Sufang
AU  - Cao S
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
FAU - Zhao, Pandeng
AU  - Zhao P
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
FAU - Wang, Haihua
AU  - Wang H
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, 
      Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220515
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein Group F-H)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 5Z93L87A1R (Guanine)
SB  - IM
MH  - Animals
MH  - Guanine
MH  - Heterogeneous-Nuclear Ribonucleoprotein Group F-H
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics/metabolism
MH  - RNA, Viral/genetics
MH  - Swine
MH  - Viral Nonstructural Proteins/metabolism
MH  - Virus Replication/physiology
PMC - PMC9107676
OTO - NOTNLM
OT  - Guanine-rich segments (GRS)
OT  - HnRNP F
OT  - Nucleocapsid (N) protein
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Replication
COIS- The authors declare that they have no competing interests.
EDAT- 2022/05/16 06:00
MHDA- 2022/05/18 06:00
PMCR- 2022/05/15
CRDT- 2022/05/15 23:22
PHST- 2022/01/09 00:00 [received]
PHST- 2022/05/05 00:00 [accepted]
PHST- 2022/05/15 23:22 [entrez]
PHST- 2022/05/16 06:00 [pubmed]
PHST- 2022/05/18 06:00 [medline]
PHST- 2022/05/15 00:00 [pmc-release]
AID - 10.1186/s12985-022-01811-4 [pii]
AID - 1811 [pii]
AID - 10.1186/s12985-022-01811-4 [doi]
PST - epublish
SO  - Virol J. 2022 May 15;19(1):82. doi: 10.1186/s12985-022-01811-4.