PMID- 35571424
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220519
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 8
DP  - 2022 Apr
TI  - Effect of acellular nerve scaffold containing human umbilical cord-derived 
      mesenchymal stem cells on nerve repair and regeneration in rats with sciatic 
      nerve defect.
PG  - 483
LID - 10.21037/atm-22-1578 [doi]
LID - 483
AB  - BACKGROUND: The aim of the present study was to investigate the effect of 
      acellular nerve scaffold (ANS) containing human umbilical cord-derived 
      mesenchymal stem cells (hUC-MSCs) on nerve repair and regeneration in rats with 
      sciatic nerve defect. METHODS: Sciatic nerve trunks were removed from 6 female 
      Sprague-Dawley (SD) rats, and ANS was prepared by lyophilization + enzymatic 
      method and divided into A, B, C, D and E groups according to different treatment 
      times. hUC-MSCs were isolated from the collected umbilical cords and cultured, 
      and then ANS-hUC-MSCs complexes were made. The other 24 adult female SD rats were 
      randomly divided into the control, autograft, ANS, and ANS-hUC-MSCs groups, and a 
      rat model of sciatic nerve defect was established. Hematoxylin-eosin (HE) 
      staining, Luxol fast blue (LFB) staining, Masson staining, and scanning electron 
      microscopy were used to observe the morphology and tissue structure of ANS. The 
      performance of ANS was evaluated by mechanical detection, and hydroxyproline 
      (HYP) content was evaluated using a biochemical kit. Flow cytometry was adopted 
      to detect the levels of hUC-MSCs surface antigens CD29, CD44, and CD34, as well 
      as electrophysiological detection and muscle wet weight recovery rate for 
      measuring rat muscle performance. RESULTS: ANS was prepared according to group A 
      method and had good mechanical properties, with less residues of cells and 
      myelin, and higher HYP content, indicating that this scaffold had the best 
      performance. ANS-hUC-MSCs significantly reduced myelin injury in the sciatic 
      nerve, and increased axonal regeneration, effectively improving sciatic nerve 
      injury in rats. In addition, ANS-hUC-MSCs significantly increased compound muscle 
      action potential (CMAP), nerve conduction velocity (NCV), and muscle wet weight, 
      and reduced muscle atrophy. CONCLUSIONS: ANS containing hUC-MSCs can promote 
      nerve repair and regeneration in rats with sciatic nerve defects.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Qian, Chuang
AU  - Qian C
AD  - Department of Orthopaedics, Children's Hospital of Fudan University and National 
      Children's Medical Center, Shanghai, China.
FAU - Zhang, Zhiqiang
AU  - Zhang Z
AD  - Department of Orthopaedics, Children's Hospital of Fudan University and National 
      Children's Medical Center, Shanghai, China.
FAU - Zhao, Rui
AU  - Zhao R
AD  - Department of Neurosurgery, Children's Hospital of Fudan University and National 
      Children's Medical Center, Shanghai, China.
FAU - Wang, Dahui
AU  - Wang D
AD  - Department of Orthopaedics, Children's Hospital of Fudan University and National 
      Children's Medical Center, Shanghai, China.
FAU - Li, Hao
AU  - Li H
AD  - Department of Neurosurgery, Children's Hospital of Fudan University and National 
      Children's Medical Center, Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC9096419
OTO - NOTNLM
OT  - Sciatic nerve defect
OT  - acellular nerve scaffold (ANS)
OT  - human umbilical cord-derived mesenchymal stem cells (hUC-MSCs)
OT  - repair and regeneration
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-1578/coif). The authors 
      have no conflicts of interest to declare.
EDAT- 2022/05/17 06:00
MHDA- 2022/05/17 06:01
PMCR- 2022/04/01
CRDT- 2022/05/16 03:58
PHST- 2022/03/08 00:00 [received]
PHST- 2022/04/20 00:00 [accepted]
PHST- 2022/05/16 03:58 [entrez]
PHST- 2022/05/17 06:00 [pubmed]
PHST- 2022/05/17 06:01 [medline]
PHST- 2022/04/01 00:00 [pmc-release]
AID - atm-10-08-483 [pii]
AID - 10.21037/atm-22-1578 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Apr;10(8):483. doi: 10.21037/atm-22-1578.