PMID- 35572691
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - A Novel Immunochromatographic Strip Based on Latex Microspheres for the Rapid 
      Detection of North American-Type Porcine Reproductive and Respiratory Syndrome 
      Virus.
PG  - 882112
LID - 10.3389/fmicb.2022.882112 [doi]
LID - 882112
AB  - A latex microsphere-based immunochromatographic strip (ICS) test was successfully 
      developed for the rapid and sensitive detection of porcine reproductive and 
      respiratory syndrome virus (PRRSV). The PRRSV N protein-specific monoclonal 
      antibody (mAb) 1H4 labeled with latex microspheres was dispensed on a conjugate 
      pad for use as the detector. The same mAb and goat anti-mouse antibody were 
      blotted on a nitrocellulose membrane to generate test and control lines, 
      respectively. The limit of virus detection was approximately 5 x 10(2.0) median 
      tissue culture infectious dose (TCID(50))/ml, and the limit of N protein 
      detection was approximately 15 ng/ml. Other common porcine viruses were tested to 
      evaluate the specificity of the ICS, and positive results were observed for only 
      North American-type PRRSV. A comparison of the strip with a standard diagnostic 
      test (reverse transcriptase polymerase chain reaction, RT-PCR) was also 
      performed, and the results showed that the ICS test exhibited relatively high 
      specificity and sensitivity (90.32 and 73.91%, respectively) and relatively high 
      positive predictive value (PPV) and negative predictive value (NPV; 85 and 
      82.35%, respectively). These results suggest that the ICS test can be used to 
      rapidly and accurately detect PRRSV and can be suitable for diagnostic 
      applications in the field.
CI  - Copyright (c) 2022 Li, Li, Zhang, Li, Xu, Gong, Fu, Guo, Peng, Zhou, Tian and Wang.
FAU - Li, Wansheng
AU  - Li W
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Li, Minhua
AU  - Li M
AD  - Beijing IDEXX Yuanheng Laboratories, Co., Ltd., Beijing, China.
FAU - Zhang, Hongliang
AU  - Zhang H
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Li, Chao
AU  - Li C
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Xu, Hu
AU  - Xu H
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Gong, Bangjun
AU  - Gong B
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Fu, Jun
AU  - Fu J
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Guo, Zhenyang
AU  - Guo Z
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Peng, Jinmei
AU  - Peng J
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Zhou, Guohui
AU  - Zhou G
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Tian, Zhijun
AU  - Tian Z
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Wang, Qian
AU  - Wang Q
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20220429
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9100670
OTO - NOTNLM
OT  - N protein
OT  - North American-type PRRSV
OT  - immunochromatographic strip
OT  - latex microsphere
OT  - monoclonal antibody
OT  - rapid detection
COIS- ML was employed by Beijing IDEXX Yuanheng Laboratories, Co., Ltd. The remaining 
      authors declare that the research was conducted in the absence of any commercial 
      or financial relationships that could be construed as a potential conflict of 
      interest.
EDAT- 2022/05/17 06:00
MHDA- 2022/05/17 06:01
PMCR- 2022/04/29
CRDT- 2022/05/16 04:15
PHST- 2022/02/23 00:00 [received]
PHST- 2022/04/11 00:00 [accepted]
PHST- 2022/05/16 04:15 [entrez]
PHST- 2022/05/17 06:00 [pubmed]
PHST- 2022/05/17 06:01 [medline]
PHST- 2022/04/29 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.882112 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Apr 29;13:882112. doi: 10.3389/fmicb.2022.882112. 
      eCollection 2022.