PMID- 35577318 OWN - NLM STAT- MEDLINE DCOM- 20220615 LR - 20220615 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 126 DP - 2022 Jul TI - The positive effects of dietary inositol on juvenile hybrid grouper (female symbol Epinephelus fuscoguttatus x male symbol E. lanceolatu) fed high-lipid diets: Growthperformance, antioxidant capacity and immunity. PG - 84-95 LID - S1050-4648(22)00256-X [pii] LID - 10.1016/j.fsi.2022.05.016 [doi] AB - The objective of the present research was to assess the influence of inositol supplementation on growth performance, histological morphology of liver, immunity and expression of immune-related genes in juvenile hybrid grouper (female symbol Epinephelus fuscoguttatus x male symbol E. lanceolatu). Hybrid grouper (initial weight 6.76 +/- 0.34 g) were fed isonitrogenous and isolipidic diets (16%) with various inositol levels of 0.17 g/kg (J1, the control group), 0.62 g/kg (J2), 1.03 g/kg (J3), 1.78 g/kg (J4), 3.43 g/kg (J5), 6.59 g/kg (J6), respectively. The growth experiment lasted for 8 weeks. The results indicated that dietary inositol had a significant promoting effect on final mean body weight of the J5 and J6 groups and specific growth rate (SGR) of the J3, J4, J5 and J6 groups (P < 0.05). In the serum, superoxide dismutase (SOD) of the J4 group became significantly active compared with that of the control group (P < 0.05), while aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (AKP) activities in the inositol-treated groups showed distinctly decreased compared with those of the control group (P < 0.05). In the liver, dietary inositol could significantly increase the activities of SOD, catalase (CAT), lysozyme (LYZ) and the contents of total antioxidative capacity (T-AOC) and immunoglobulin M (IgM) (P < 0.05), and distinctly reduce the content of malondialdehyde (MDA) as well as reactive oxygen species (ROS) (P < 0.05). Compared with the control group, the damaged histological morphology of the liver was relieved and even returned to normal after an inositol increase (0.4-3.2 g/kg). In the liver, the remarkable up-regulation of SOD, CAT, glutathione peroxidase (GPX), heat shock protein70 (HSP70) and heat shock protein90 (HSP90) expression levels were stimulated by supply of inositol, while interleukin 6 (IL6), interleukin 8 (IL8) and transforming growth factor beta (TGF-beta) expression levels were down-regulated by supply of inositol. In head kidney, the mRNA of toll-like receptor 22 (TLR22), myeloid differentiation factor 88 (MyD88) and interleukin 1beta (IL1beta) expression levels were significantly down-regulated (P < 0.05), which could further lead to remarkable down-regulation of IL6 and tumor necrosis factor alpha (TNF-alpha) expression (P < 0.05). These results indicated that high-lipid diets with supply of inositol promoted growth, increased the antioxidant capacity, and suppressed the inflammation of the liver and head kidney by inhibiting the expression of pro-inflammation factors (IL6, IL8, TGF-beta and TNF-alpha). In conclusion, these results indicated that dietary inositol promoted growth, improved antioxidant capacity and immunity of hybrid grouper fed high-lipid diets. Based on SGR, broken-line regression analysis showed that 1.66 g/kg inositol supply was recommended in high-lipid diets of juvenile grouper. CI - Copyright (c) 2022 Elsevier Ltd. All rights reserved. FAU - Pan, Simiao AU - Pan S AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China. FAU - Yan, Xiaobo AU - Yan X AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China. FAU - Dong, Xiaohui AU - Dong X AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong, 524000, China. Electronic address: dongxiaohui2003@163.com. FAU - Li, Tao AU - Li T AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China. FAU - Suo, Xiangxiang AU - Suo X AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China. FAU - Tan, Beiping AU - Tan B AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong, 524000, China. FAU - Zhang, Shuang AU - Zhang S AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong, 524000, China. FAU - Li, Zhihao AU - Li Z AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, Guangdong, 524088, China. FAU - Yang, Yuanzhi AU - Yang Y AD - Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China. FAU - Zhang, Haitao AU - Zhang H AD - Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong, 524000, China. LA - eng PT - Journal Article DEP - 20220513 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Antioxidants) RN - 0 (Interleukin-6) RN - 0 (Interleukin-8) RN - 0 (Lipids) RN - 0 (Transforming Growth Factor beta) RN - 0 (Tumor Necrosis Factor-alpha) RN - 4L6452S749 (Inositol) RN - EC 1.15.1.1 (Superoxide Dismutase) SB - IM MH - Animal Feed/analysis MH - Animals MH - Antioxidants/metabolism MH - *Bass/genetics MH - Diet/veterinary MH - Dietary Supplements/analysis MH - Immunity, Innate/genetics MH - Inflammation MH - Inositol/pharmacology MH - Interleukin-6 MH - Interleukin-8 MH - Lipids MH - Superoxide Dismutase/pharmacology MH - Transforming Growth Factor beta MH - Tumor Necrosis Factor-alpha/pharmacology OTO - NOTNLM OT - Growth OT - High-lipid diets OT - Immunity OT - Inositol OT - female symbol Epinephelus fuscoguttatus x male symbol E. lanceolatu EDAT- 2022/05/17 06:00 MHDA- 2022/06/16 06:00 CRDT- 2022/05/16 19:37 PHST- 2022/02/18 00:00 [received] PHST- 2022/05/06 00:00 [revised] PHST- 2022/05/08 00:00 [accepted] PHST- 2022/05/17 06:00 [pubmed] PHST- 2022/06/16 06:00 [medline] PHST- 2022/05/16 19:37 [entrez] AID - S1050-4648(22)00256-X [pii] AID - 10.1016/j.fsi.2022.05.016 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2022 Jul;126:84-95. doi: 10.1016/j.fsi.2022.05.016. Epub 2022 May 13.