PMID- 35578895
OWN - NLM
STAT- MEDLINE
DCOM- 20220801
LR  - 20220902
IS  - 1460-2091 (Electronic)
IS  - 0305-7453 (Linking)
VI  - 77
IP  - 8
DP  - 2022 Jul 28
TI  - In vitro interactions within a biofilm containing three species found in 
      bacterial vaginosis (BV) support the higher antimicrobial tolerance associated 
      with BV recurrence.
PG  - 2183-2190
LID - 10.1093/jac/dkac155 [doi]
AB  - BACKGROUND: Bacterial vaginosis (BV), the most common cause of vaginal discharge, 
      is characterized by the presence of a polymicrobial biofilm on the vaginal 
      epithelium, formed primarily by Gardnerella spp., but also other anaerobic 
      species. Interactions between bacteria in multi-species biofilms are likely to 
      contribute to increased virulence and to enhanced antimicrobial tolerance 
      observed in vivo. However, functional studies addressing this question are 
      lacking. OBJECTIVES: To gain insights into the role that interactions between 
      BV-associated species in multi-species BV biofilms might have on antimicrobial 
      tolerance, single- and triple-species biofilms formed by Gardnerella vaginalis, 
      Fannyhessea (Atopobium) vaginae and Peptostreptococcus anaerobius were 
      characterized, before and after metronidazole or clindamycin treatment. METHODS: 
      Total biofilm biomass, total cells and cfu counts prior to and after antibiotic 
      treatment were first determined. In addition, bacterial populations in the 
      triple-species biofilms were also quantified by quantitative PCR (qPCR) and 
      peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH). RESULTS: 
      Despite the effect observed in single-species biofilms, neither metronidazole nor 
      clindamycin was effective in reducing triple-species biofilm biomass. Similar 
      results were obtained when evaluating the number of total or culturable cells. 
      Interestingly, despite differences between strain susceptibilities to 
      antibiotics, the composition of the triple-species biofilms was not strongly 
      affected by antibiotics. CONCLUSIONS: Taken together, these results strengthen 
      the idea that, when co-incubated, bacteria can interact synergistically, leading 
      to increased tolerance to antimicrobial therapy, which helps explain the observed 
      clinically high BV recurrence rates.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of British 
      Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, 
      please e-mail: journals.permissions@oup.com.
FAU - Rosca, Aliona S
AU  - Rosca AS
AD  - Laboratory of Research in Biofilms Rosario Oliveira (LIBRO), Centre of Biological 
      Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, 
      Portugal.
AD  - Laboratory of Bacteriology Research (LBR), Faculty of Medicine and Health 
      Sciences, Ghent University, 9000 Ghent, Belgium.
FAU - Castro, Joana
AU  - Castro J
AD  - Laboratory of Research in Biofilms Rosario Oliveira (LIBRO), Centre of Biological 
      Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, 
      Portugal.
FAU - Sousa, Lucia G V
AU  - Sousa LGV
AD  - Laboratory of Research in Biofilms Rosario Oliveira (LIBRO), Centre of Biological 
      Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, 
      Portugal.
AD  - LABBELS - Associate Laboratory, Braga/Guimaraes, Portugal.
FAU - Franca, Angela
AU  - Franca A
AUID- ORCID: 0000-0001-7117-6837
AD  - Laboratory of Research in Biofilms Rosario Oliveira (LIBRO), Centre of Biological 
      Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, 
      Portugal.
AD  - LABBELS - Associate Laboratory, Braga/Guimaraes, Portugal.
FAU - Vaneechoutte, Mario
AU  - Vaneechoutte M
AD  - Laboratory of Bacteriology Research (LBR), Faculty of Medicine and Health 
      Sciences, Ghent University, 9000 Ghent, Belgium.
FAU - Cerca, Nuno
AU  - Cerca N
AUID- ORCID: 0000-0003-3365-3537
AD  - Laboratory of Research in Biofilms Rosario Oliveira (LIBRO), Centre of Biological 
      Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, 
      Portugal.
AD  - LABBELS - Associate Laboratory, Braga/Guimaraes, Portugal.
LA  - eng
GR  - Portuguese Foundation for Science and Technology/
GR  - PTDC/BIA-MIC/28271/2017/FCT/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Infective Agents)
RN  - 140QMO216E (Metronidazole)
RN  - 3U02EL437C (Clindamycin)
RN  - Atopobium vaginae
SB  - IM
MH  - Actinobacteria
MH  - Anti-Bacterial Agents/pharmacology
MH  - *Anti-Infective Agents
MH  - Bacteria
MH  - Biofilms
MH  - Clindamycin/pharmacology
MH  - Female
MH  - Gardnerella vaginalis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metronidazole/pharmacology
MH  - Vagina/microbiology
MH  - *Vaginosis, Bacterial/microbiology
EDAT- 2022/05/18 06:00
MHDA- 2022/08/02 06:00
CRDT- 2022/05/17 03:54
PHST- 2021/12/01 00:00 [received]
PHST- 2022/04/22 00:00 [accepted]
PHST- 2022/05/18 06:00 [pubmed]
PHST- 2022/08/02 06:00 [medline]
PHST- 2022/05/17 03:54 [entrez]
AID - 6586622 [pii]
AID - 10.1093/jac/dkac155 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2022 Jul 28;77(8):2183-2190. doi: 10.1093/jac/dkac155.