PMID- 35579787
OWN - NLM
STAT- MEDLINE
DCOM- 20220718
LR  - 20220727
IS  - 1573-7365 (Electronic)
IS  - 0885-7490 (Linking)
VI  - 37
IP  - 6
DP  - 2022 Aug
TI  - NGF/TrkA promotes the vitality, migration and adhesion of bone marrow stromal 
      cells in hypoxia by regulating the Nrf2 pathway.
PG  - 2017-2026
LID - 10.1007/s11011-022-00974-x [doi]
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) transplantation is a treatment 
      strategy for ischemic stroke (IS) with great potential. However, the vitality, 
      migration and adhesion of BMSCs are greatly impaired due to the harsh environment 
      of the ischemic area, which affects the therapeutic effects. Herein, we aimed to 
      investigate the roles of nerve growth factor (NGF) in regulating cell behaviors 
      of BMSCs in IS. METHODS: The mRNA and protein expressions were assessed using 
      qRT-PCR and western blot, respectively. To simulate ischemic-like conditions in 
      vitro, Brain microvascular (bEnd.3) cells were exposed to oxygen and glucose 
      deprivation (OGD). Cell viability and cell proliferation were evaluated by MTT 
      assay and BrdU assay, respectively. Transwell migration and cell adhesion assays 
      were carried out to determine cell migration and adhesion of BMSCs, respectively, 
      coupled with flow cytometry to evaluate cell apoptosis of bEnd.3 cells. Finally, 
      angiogenesis assay was performed to assess the angiogenesis ability of bEnd.3 
      cells. RESULTS: NGF overexpression resulted in increased cell vitality, adhesion 
      and migration of BMSCs, while NGF knockdown presented the opposite effects. We 
      subsequently discovered that TrkA was a receptor for NGF, and TrkA knockdown 
      significantly inhibited the cell viability, migration and adhesion of BMSCs. 
      Besides, Nrf2 was confirmed as the downstream target of NGF/TrkA to promote the 
      viability, adhesion and migration of BMSC cells. Finally, NGF-silenced BMSCs 
      could not effectively restore the OGD-induced brain microvascular cell damage. 
      CONCLUSIONS: NGF/TrkA promoted the viability, migration and adhesion of BMSCs in 
      IS via activating Nrf2 pathway.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Fang, Cui-Ni
AU  - Fang CN
AD  - Department of Rehabilitation, Hunan Provincial People's Hospital (the 
      first-affiliated Hospital of Hunan normal University), No.89, Guhan Road, Furong 
      District, 410000, Changsha, Hunan Province, P.R. China.
FAU - Tan, Hai-Qun
AU  - Tan HQ
AD  - Department of Rehabilitation, Hunan Provincial People's Hospital (the 
      first-affiliated Hospital of Hunan normal University), No.89, Guhan Road, Furong 
      District, 410000, Changsha, Hunan Province, P.R. China.
FAU - Song, Ao-Bo
AU  - Song AB
AD  - Department of Rehabilitation, Hunan Provincial People's Hospital (the 
      first-affiliated Hospital of Hunan normal University), No.89, Guhan Road, Furong 
      District, 410000, Changsha, Hunan Province, P.R. China.
FAU - Jiang, Ni
AU  - Jiang N
AD  - Department of Rehabilitation, Hunan Provincial People's Hospital (the 
      first-affiliated Hospital of Hunan normal University), No.89, Guhan Road, Furong 
      District, 410000, Changsha, Hunan Province, P.R. China.
FAU - Liu, Qian-Rong
AU  - Liu QR
AD  - Department of Rehabilitation, Hunan Provincial People's Hospital (the 
      first-affiliated Hospital of Hunan normal University), No.89, Guhan Road, Furong 
      District, 410000, Changsha, Hunan Province, P.R. China.
FAU - Song, Tao
AU  - Song T
AUID- ORCID: 0000-0002-3259-2456
AD  - Department of Rehabilitation, Hunan Provincial People's Hospital (the 
      first-affiliated Hospital of Hunan normal University), No.89, Guhan Road, Furong 
      District, 410000, Changsha, Hunan Province, P.R. China. songtao0731@126.com.
LA  - eng
PT  - Journal Article
DEP - 20220517
PL  - United States
TA  - Metab Brain Dis
JT  - Metabolic brain disease
JID - 8610370
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NTRK1 protein, human)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cells, Cultured
MH  - Endothelial Cells/metabolism
MH  - Humans
MH  - Hypoxia
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - NF-E2-Related Factor 2/metabolism
MH  - *Nerve Growth Factor/metabolism
MH  - Receptor, trkA
OTO - NOTNLM
OT  - Bone marrow stromal cells
OT  - Cell adhesion
OT  - Cell migration
OT  - Ischemic stroke
OT  - NGF/TrkA
EDAT- 2022/05/18 06:00
MHDA- 2022/07/19 06:00
CRDT- 2022/05/17 11:18
PHST- 2021/11/09 00:00 [received]
PHST- 2022/03/24 00:00 [accepted]
PHST- 2022/05/18 06:00 [pubmed]
PHST- 2022/07/19 06:00 [medline]
PHST- 2022/05/17 11:18 [entrez]
AID - 10.1007/s11011-022-00974-x [pii]
AID - 10.1007/s11011-022-00974-x [doi]
PST - ppublish
SO  - Metab Brain Dis. 2022 Aug;37(6):2017-2026. doi: 10.1007/s11011-022-00974-x. Epub 
      2022 May 17.