PMID- 35580574
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20220527
IS  - 1464-3553 (Electronic)
IS  - 0379-864X (Linking)
VI  - 47
DP  - 2022 Jan 1
TI  - Green spotted puffers detect a nontoxic TTX analog odor using crypt olfactory 
      sensory neurons.
LID - bjac011 [pii]
LID - 10.1093/chemse/bjac011 [doi]
AB  - Toxic puffers accumulate their defense substance (tetrodotoxin; TTX) through the 
      food chain. Although the previous study suggests that 5,6,11-trideoxyTTX, a 
      nontoxic TTX analog detected simultaneously with TTX in toxic puffers or their 
      prey, acts as an olfactory chemoattractant for grass puffers, it is unclear 
      whether toxic puffers are commonly attracted to 5,6,11-trideoxyTTX, and which 
      types of olfactory sensory neurons (OSNs) detect 5,6,11-trideoxyTTX. Here, we 
      demonstrated that green spotted puffer, a phylogenetically distant species from 
      the grass puffer, is attracted to 5,6,11-trideoxyTTX. 5,6,11-TrideoxyTTX 
      administration made green spotted puffers stay longer at the administered site, 
      whereas a food odor (l-Arg) made them actively swim throughout the aquarium. 
      Attractive responses were not observed when TTX or its vehicle was administered, 
      nor when 5,6,11-trideoxyTTX was administered to anosmic fish. Furthermore, double 
      immunohistochemistry with activity marker and crypt OSN marker antibodies labeled 
      oval cells with apical invagination on the olfactory epithelium surface treated 
      with 5,6,11-trideoxyTTX. These results suggest that 5,6,11-trideoxyTTX acts as an 
      olfactory chemoattractant detected by crypt OSNs, and attraction to 
      5,6,11-trideoxyTTX odor appears to be a trait shared by toxic puffers for social 
      communication or effective toxification.
CI  - (c) The Author(s) 2022. Published by Oxford University Press. All rights reserved. 
      For permissions, please e-mail: journals.permissions@oup.com.
FAU - Suzuki, Takehisa
AU  - Suzuki T
AUID- ORCID: 0000-0003-0755-4324
AD  - Laboratory of Fish Biology, Department of Animal Sciences, Graduate School of 
      Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 
      464-8601, Japan.
FAU - Nakahigashi, Ryota
AU  - Nakahigashi R
AD  - Laboratory of Organic Chemistry, Department of Applied Biosciences, Graduate 
      School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, 
      Nagoya, Aichi 464-8601, Japan.
FAU - Adachi, Masaatsu
AU  - Adachi M
AUID- ORCID: 0000-0001-7615-3861
AD  - Laboratory of Organic Chemistry, Department of Applied Biosciences, Graduate 
      School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, 
      Nagoya, Aichi 464-8601, Japan.
FAU - Nishikawa, Toshio
AU  - Nishikawa T
AUID- ORCID: 0000-0003-1955-6618
AD  - Laboratory of Organic Chemistry, Department of Applied Biosciences, Graduate 
      School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, 
      Nagoya, Aichi 464-8601, Japan.
FAU - Abe, Hideki
AU  - Abe H
AUID- ORCID: 0000-0002-8931-5767
AD  - Laboratory of Fish Biology, Department of Animal Sciences, Graduate School of 
      Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 
      464-8601, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Chem Senses
JT  - Chemical senses
JID - 8217190
RN  - 0 (Chemotactic Factors)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Animals
MH  - Chemotactic Factors
MH  - Odorants
MH  - Olfactory Mucosa
MH  - *Olfactory Receptor Neurons
MH  - *Tetraodontiformes
MH  - Tetrodotoxin/chemistry/pharmacology
OTO - NOTNLM
OT  - activity labeling
OT  - attractive behavior
OT  - chemical communication
OT  - chemoattractant
OT  - pheromone
OT  - toxification
EDAT- 2022/05/18 06:00
MHDA- 2022/05/21 06:00
CRDT- 2022/05/17 18:32
PHST- 2022/05/17 18:32 [entrez]
PHST- 2022/05/18 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
AID - 6586859 [pii]
AID - 10.1093/chemse/bjac011 [doi]
PST - ppublish
SO  - Chem Senses. 2022 Jan 1;47:bjac011. doi: 10.1093/chemse/bjac011.