PMID- 35580782
OWN - NLM
STAT- MEDLINE
DCOM- 20220810
LR  - 20220830
IS  - 1878-8769 (Electronic)
IS  - 1878-8750 (Linking)
VI  - 164
DP  - 2022 Aug
TI  - Impinging Flow Induces Expression of Monocyte Chemoattractant Protein-1 in 
      Endothelial Cells Through Activation of the c-Jun N-terminal 
      Kinase/c-Jun/p38/c-Fos Pathway.
PG  - e681-e693
LID - S1878-8750(22)00663-5 [pii]
LID - 10.1016/j.wneu.2022.05.032 [doi]
AB  - OBJECTIVE: Monocyte chemoattractant protein-1 (MCP-1) is an important regulator 
      of the formation and development of intracranial aneurysms. This study explored 
      the molecular mechanisms underlying the induction of MCP-1 and related 
      inflammatory factors in human umbilical vein endothelial cells (HUVECs) under 
      hemodynamic conditions. METHODS: A modified T chamber was used to simulate fluid 
      flow at the bifurcation of the artery and wall shear stress on HUVECs in vitro. 
      Changes in HUVECs were analyzed in response to impinging flow. And HUVECs without 
      impinging flow were used as the control group. Protein expression levels of 
      extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38, 
      activator protein-1, and MCP-1 were detected by Western blot, and the messenger 
      RNA expression levels of MCP-1, interleukin (IL)-1beta, and IL-6 were determined by 
      quantitative reverse transcription polymerase chain reaction. RESULTS: Under 
      impinging flow, the phosphorylation levels of ERK, JNK, and p38, as well as the 
      protein levels of MCP-1, c-Jun, and c-Fos, increased. The messenger RNA 
      expression of MCP-1, IL-1beta, and IL-6 also increased in HUVECs. Pretreatment of 
      the HUVECs with inhibitors of JNK and p38 significantly attenuated the increased 
      expression of MCP-1, IL-1beta, and IL-6, while ERK inhibitors had no obvious effect. 
      CONCLUSIONS: Under impinging flow, MCP-1 and inflammatory factors are regulated 
      through the JNK/c-Jun/p38/c-Fos pathway and participate in EC inflammation.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Zhu, Huaxin
AU  - Zhu H
AD  - Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi, China.
FAU - Hao, Zheng
AU  - Hao Z
AD  - Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi, China; Trauma Center, the First Affiliated Hospital of 
      Nanchang University, Nanchang, Jiangxi, China.
FAU - Xing, Zelong
AU  - Xing Z
AD  - Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi, China; Department of Neurosurgery, Jiujiang University 
      Affiliated Hospital, Jiujiang, Jiangxi, China.
FAU - Tan, Jiacong
AU  - Tan J
AD  - Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi, China.
FAU - Zhao, Yeyu
AU  - Zhao Y
AD  - Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi, China.
FAU - Li, Meihua
AU  - Li M
AD  - Department of Neurosurgery, the First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi, China. Electronic address: limeihua2000@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220514
PL  - United States
TA  - World Neurosurg
JT  - World neurosurgery
JID - 101528275
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Cells, Cultured
MH  - *Chemokine CCL2/genetics/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - *JNK Mitogen-Activated Protein Kinases/metabolism
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - HUVECs
OT  - Hemodynamics
OT  - JNK
OT  - MCP-1
OT  - p38
EDAT- 2022/05/18 06:00
MHDA- 2022/08/11 06:00
CRDT- 2022/05/17 19:25
PHST- 2022/05/04 00:00 [received]
PHST- 2022/05/09 00:00 [accepted]
PHST- 2022/05/18 06:00 [pubmed]
PHST- 2022/08/11 06:00 [medline]
PHST- 2022/05/17 19:25 [entrez]
AID - S1878-8750(22)00663-5 [pii]
AID - 10.1016/j.wneu.2022.05.032 [doi]
PST - ppublish
SO  - World Neurosurg. 2022 Aug;164:e681-e693. doi: 10.1016/j.wneu.2022.05.032. Epub 
      2022 May 14.