PMID- 35582406
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240115
IS  - 2214-0883 (Electronic)
IS  - 2095-1779 (Print)
IS  - 2214-0883 (Linking)
VI  - 12
IP  - 2
DP  - 2022 Apr
TI  - GB7 acetate, a galbulimima alkaloid from Galbulimima belgraveana, possesses 
      anticancer effects in colorectal cancer cells.
PG  - 339-349
LID - 10.1016/j.jpha.2021.06.007 [doi]
AB  - GB7 acetate is a galbulimima alkaloid obtained from Galbulimima belgraveana. 
      However, information regarding its structure, biological activities, and related 
      mechanisms is not entirely available. A series of spectroscopic analyses, 
      structural degradation, interconversion, and crystallography were performed to 
      identify the structure of GB7 acetate. The MTT assay was applied to measure cell 
      proliferation on human colorectal cancer HCT 116 cells. The expressions of the 
      related proteins were measured by Western blotting. Transmission electron 
      microscopy (TEM), acridine orange (AO) and monodansylcadaverine (MDC) staining 
      were used to detect the presence of autophagic vesicles and autolysosomes. A 
      transwell assay was performed to demonstrate metastatic capabilities. Oxygen 
      consumption rate (OCR) and extracellular acidification rate (ECAR) assays were 
      performed to determine the mitochondrial oxidative phosphorylation (OXPHOS) and 
      glycolysis activity of HCT 116 cells. The data showed that GB7 acetate suppressed 
      the proliferation and colony-forming ability of HCT 116 cells. Pretreatment with 
      GB7 acetate significantly induced the formation of autophagic vesicles and 
      autolysosomes. GB7 acetate upregulated the expressions of LC3 and Thr172 
      phosphorylated adenosine 5'-monophosphate (AMP)-activated protein kinase alpha 
      (p-AMPKalpha), which are key elements of autophagy. In addition, GB7 acetate 
      suppressed the metastatic capabilities of HCT 116 cells. Additionally, the 
      production of matrix metallo-proteinase-2 (MMP-2) and MMP-9 was reduced, whereas 
      the expression of E-cadherin (E-cad) was upregulated. Furthermore, GB7 acetate 
      significantly reduced mitochondrial OXPHOS and glycolysis. In conclusion, the 
      structure of the novel Galbulimima alkaloid GB7 acetate was identified. GB7 
      acetate was shown to have anti-proliferative, pro-autophagic, anti-metastatic, 
      and anti-metabolite capabilities in HCT 116 cells. This study might provide new 
      insights into cancer treatment efficacy and cancer chemoprevention.
CI  - (c) 2021 The Authors.
FAU - Li, Ziyin
AU  - Li Z
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Mao, Lianzhi
AU  - Mao L
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Yu, Bin
AU  - Yu B
AD  - School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450000, 
      China.
FAU - Liu, Huahuan
AU  - Liu H
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Zhang, Qiuyu
AU  - Zhang Q
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Bian, Zhongbo
AU  - Bian Z
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Zhang, Xudong
AU  - Zhang X
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Liao, Wenzhen
AU  - Liao W
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
FAU - Sun, Suxia
AU  - Sun S
AD  - Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of 
      Tropical Disease Research, School of Public Health, Southern Medical University, 
      Guangzhou, 510515, China.
LA  - eng
PT  - Journal Article
DEP - 20210627
PL  - China
TA  - J Pharm Anal
JT  - Journal of pharmaceutical analysis
JID - 101579451
EIN - J Pharm Anal. 2023 Dec;13(12):1580-1582. PMID: 38223445
PMC - PMC9091789
OTO - NOTNLM
OT  - Anti-cancer effects
OT  - GB7 acetate
OT  - Galbulimima belgraveana
OT  - HCT 116 cells
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2022/05/19 06:00
MHDA- 2022/05/19 06:01
PMCR- 2021/06/27
CRDT- 2022/05/18 02:09
PHST- 2020/07/13 00:00 [received]
PHST- 2021/06/23 00:00 [revised]
PHST- 2021/06/24 00:00 [accepted]
PHST- 2022/05/18 02:09 [entrez]
PHST- 2022/05/19 06:00 [pubmed]
PHST- 2022/05/19 06:01 [medline]
PHST- 2021/06/27 00:00 [pmc-release]
AID - S2095-1779(21)00067-8 [pii]
AID - 10.1016/j.jpha.2021.06.007 [doi]
PST - ppublish
SO  - J Pharm Anal. 2022 Apr;12(2):339-349. doi: 10.1016/j.jpha.2021.06.007. Epub 2021 
      Jun 27.