PMID- 35584505
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20220716
IS  - 1678-7765 (Electronic)
IS  - 1678-7757 (Print)
IS  - 1678-7757 (Linking)
VI  - 30
DP  - 2022
TI  - Decreased CD1a + and CD83 + cells in tonsillar squamous cell carcinoma regardless 
      of HPV status.
PG  - e20210702
LID - S1678-77572022000100425 [pii]
LID - 10.1590/1678-7757-2020-0702 [doi]
LID - e20210702
AB  - BACKGROUND: Dendritic cells (DCs) are specialized antigen-presenting cells that 
      play a critical role in the immune response against human papillomavirus (HPV) 
      infection, and represent a therapeutic target in cancer. OBJECTIVE: To identify 
      and quantify DCs in tonsillar squamous cell carcinoma (TSCC) under the influence 
      of HPV infection. METHODOLOGY: CD1a and CD83 antibodies were used to identify 
      immature dendritic cells and mature dendritic cells by immunohistochemistry in 33 
      primary TSCC and 10 normal tonsils (NTs), respectively. For the TSCC samples, the 
      number of DCs per area was evaluated in the intra- and peritumoral compartments. 
      For the NTs, the quantification of DCs was evaluated in the intra- and 
      peritonsillar compartments. HPV detection methods were determined according to 
      the ASCO Clinical Practice Guidelines from the College of American Pathologists 
      Guideline (2018). RESULTS: There were fewer intratumoral CD1a+ DCs in the 
      HPV-positive and HPV-negative TSCC groups than in the NT group (p<0.05). In the 
      peritumoral compartment, there were fewer CD83+ DCs in the HPV-positive and 
      HPV-negative TSCC groups than in the NT group (p<0.001). The quantification of 
      DCs subtypes showed no statistical differences between HPV-positive and 
      HPV-negative TSCC groups (p>0.137). Patients with HPV-positive TSCC had 
      significantly better overall survival rate than those with HPV-negative TSCC 
      (p=0.004). CONCLUSION: Tumor activity contributes to DC depletion regardless of 
      intralesional HPV positivity. An improved prognosis has been reported in patients 
      with HPV-positive TSCC.
FAU - Gama-Cuellar, Ana Guadalupe
AU  - Gama-Cuellar AG
AUID- ORCID: 0000-0002-4410-3322
AD  - Universidade Federal de Santa Catarina - UFSC, Departamento de Patologia, 
      Florianopolis, SC, Brasil.
FAU - Francisco, Ana Lucia Noronha
AU  - Francisco ALN
AUID- ORCID: 0000-0003-0408-614X
AD  - Hospital AC Camargo, Servico de Cabeca e Pescoco, Sao Paulo, SP, Brasil.
FAU - Scarini, Joao Figueira
AU  - Scarini JF
AUID- ORCID: 0000-0001-9015-6831
AD  - Universidade Estadual de Campinas - UNICAMP, Departamento de Anatomia-Patologica, 
      Faculdade de Ciencias Medicas, Campinas, SP, Brasil.
FAU - Mariano, Fernanda Viviane
AU  - Mariano FV
AUID- ORCID: 0000-0003-3694-5025
AD  - Universidade Estadual de Campinas - UNICAMP, Departamento de Anatomia-Patologica, 
      Faculdade de Ciencias Medicas, Campinas, SP, Brasil.
FAU - Kowalski, Luiz Paulo
AU  - Kowalski LP
AUID- ORCID: 0000-0002-0481-156X
AD  - Hospital AC Camargo, Servico de Cabeca e Pescoco, Sao Paulo, SP, Brasil.
FAU - Gondak, Rogerio
AU  - Gondak R
AUID- ORCID: 0000-0001-7547-660X
AD  - Universidade Federal de Santa Catarina - UFSC, Departamento de Patologia, 
      Florianopolis, SC, Brasil.
LA  - eng
PT  - Journal Article
DEP - 20220516
PL  - Brazil
TA  - J Appl Oral Sci
JT  - Journal of applied oral science : revista FOB
JID - 101189774
SB  - IM
MH  - *Carcinoma, Squamous Cell/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - *Papillomavirus Infections
MH  - Prognosis
MH  - *Tonsillar Neoplasms/pathology
PMC - PMC9126112
COIS- Conflict of interest The authors declare no conflict of interest.
EDAT- 2022/05/19 06:00
MHDA- 2022/05/21 06:00
PMCR- 2022/05/16
CRDT- 2022/05/18 17:04
PHST- 2021/12/22 00:00 [received]
PHST- 2022/04/06 00:00 [accepted]
PHST- 2022/05/18 17:04 [entrez]
PHST- 2022/05/19 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2022/05/16 00:00 [pmc-release]
AID - S1678-77572022000100425 [pii]
AID - 10.1590/1678-7757-2020-0702 [doi]
PST - epublish
SO  - J Appl Oral Sci. 2022 May 16;30:e20210702. doi: 10.1590/1678-7757-2020-0702. 
      eCollection 2022.