PMID- 35584545
OWN - NLM
STAT- MEDLINE
DCOM- 20220622
LR  - 20220818
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 5
IP  - 6
DP  - 2022 Jun 20
TI  - Fabrication of Radio-Opaque and Macroporous Injectable Calcium Phosphate Cement.
PG  - 3075-3085
LID - 10.1021/acsabm.2c00345 [doi]
AB  - The aim of this work was the development of injectable radio-opaque and 
      macroporous calcium phosphate cement (CPC) to be used as a bone substitute for 
      the treatment of pathologic vertebral fractures. A CPC was first rendered 
      radio-opaque by the incorporation of zirconium dioxide (ZrO(2)). In order to 
      create macroporosity, poly lactic-co-glycolic acid (PLGA) microspheres around 100 
      mum were homogeneously incorporated into the CPC as observed by scanning electron 
      microscopy. Physicochemical analyses by X-ray diffraction and Fourier transform 
      infrared spectroscopy confirmed the brushite phase of the cement. The mechanical 
      properties of the CPC/PLGA cement containing 30% PLGA (wt/wt) were characterized 
      by a compressive strength of 2 MPa and a Young's modulus of 1 GPa. The CPC/PLGA 
      exhibited initial and final setting times of 7 and 12 min, respectively. Although 
      the incorporation of PLGA microspheres increased the force necessary to inject 
      the cement and decreased the percentage of injected mass as a function of time, 
      the CPC/PLGA appeared fully injectable at 4 min. Moreover, in comparison with 
      CPC, CPC/PLGA showed a full degradation in 6 weeks (with 100% mass loss), and 
      this was associated with an acidification of the medium containing the CPC/PLGA 
      sample (pH of 3.5 after 6 weeks). A cell viability test validated CPC/PLGA 
      biocompatibility, and in vivo analyses using a bone defect assay in the caudal 
      vertebrae of Wistar rats showed the good opacity of the CPC through the tail and 
      a significant increased degradation of the CPC/PLGA cement a month after 
      implantation. In conclusion, this injectable CPC scaffold appears to be an 
      interesting material for bone substitution.
FAU - Belaid, Habib
AU  - Belaid H
AD  - Institut Europeen des Membranes, IEM, UMR 5635, Univ Montpellier, CNRS, ENSCM, 
      34095 Montpellier, France.
AD  - IRCM, Institut de Recherche en Cancerologie de Montpellier, INSERM U1194, 
      Universite Montpellier, F-34298 Montpellier, France.
FAU - Barou, Carole
AU  - Barou C
AD  - Institut Europeen des Membranes, IEM, UMR 5635, Univ Montpellier, CNRS, ENSCM, 
      34095 Montpellier, France.
AD  - IRCM, Institut de Recherche en Cancerologie de Montpellier, INSERM U1194, 
      Universite Montpellier, F-34298 Montpellier, France.
AD  - Biologics 4 Life, 84120 Pertuis, France.
FAU - Collart-Dutilleul, Pierre-Yves
AU  - Collart-Dutilleul PY
AD  - Laboratoire de Bioingenierie et Nanosciences, EA4203, Universite de Montpellier, 
      34193 Montpellier, France.
FAU - Desoutter, Alban
AU  - Desoutter A
AD  - Laboratoire de Bioingenierie et Nanosciences, EA4203, Universite de Montpellier, 
      34193 Montpellier, France.
FAU - Kajdan, Marilyn
AU  - Kajdan M
AD  - IRCM, Institut de Recherche en Cancerologie de Montpellier, INSERM U1194, 
      Universite Montpellier, F-34298 Montpellier, France.
FAU - Bernex, Florence
AU  - Bernex F
AD  - IRCM, Institut de Recherche en Cancerologie de Montpellier, INSERM U1194, 
      Universite Montpellier, F-34298 Montpellier, France.
AD  - BioCampus, RHEM, Universite de Montpellier, CNRS UAR3426, INSERM, F-34298 
      Montpellier, France.
FAU - Tetreau, Raphael
AU  - Tetreau R
AD  - Service d'Imagerie, Institut Regional du Cancer Montpellier, Montpellier F-34298, 
      France.
FAU - Cuisinier, Frederic
AU  - Cuisinier F
AUID- ORCID: 0000-0001-6870-0490
AD  - Laboratoire de Bioingenierie et Nanosciences, EA4203, Universite de Montpellier, 
      34193 Montpellier, France.
FAU - Bares, Jonathan
AU  - Bares J
AD  - Laboratoire de Mecanique et Genie Civil, Univ Montpellier, CNRS, Montpellier 
      34090, France.
FAU - Huon, Vincent
AU  - Huon V
AD  - Laboratoire de Mecanique et Genie Civil, Univ Montpellier, CNRS, Montpellier 
      34090, France.
FAU - Teyssier, Catherine
AU  - Teyssier C
AD  - IRCM, Institut de Recherche en Cancerologie de Montpellier, INSERM U1194, 
      Universite Montpellier, F-34298 Montpellier, France.
FAU - Cornu, David
AU  - Cornu D
AD  - Institut Europeen des Membranes, IEM, UMR 5635, Univ Montpellier, CNRS, ENSCM, 
      34095 Montpellier, France.
FAU - Cavailles, Vincent
AU  - Cavailles V
AD  - IRCM, Institut de Recherche en Cancerologie de Montpellier, INSERM U1194, 
      Universite Montpellier, F-34298 Montpellier, France.
FAU - Bechelany, Mikhael
AU  - Bechelany M
AUID- ORCID: 0000-0002-2913-2846
AD  - Institut Europeen des Membranes, IEM, UMR 5635, Univ Montpellier, CNRS, ENSCM, 
      34095 Montpellier, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220518
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Bone Cements/pharmacology
MH  - Calcium Phosphates/chemistry
MH  - *Lactic Acid
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/chemistry
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - PLGA microspheres
OT  - bone metastasis
OT  - calcium phosphate cement
OT  - radio-opaque
OT  - vertebroplasty
EDAT- 2022/05/19 06:00
MHDA- 2022/06/23 06:00
CRDT- 2022/05/18 17:52
PHST- 2022/05/19 06:00 [pubmed]
PHST- 2022/06/23 06:00 [medline]
PHST- 2022/05/18 17:52 [entrez]
AID - 10.1021/acsabm.2c00345 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2022 Jun 20;5(6):3075-3085. doi: 10.1021/acsabm.2c00345. Epub 
      2022 May 18.